Team:Mingdao/HP/Silver

iGEM Human Practice

Overview

"Human Practices is the study of how your work affects the world, and how the world affects your work." — Peter Carr, Director of Judging. What’s Human practice? It is not just a work  to confirm and strengthen what we think is right. Instead, we should learn to share our knowledge with the world. We can’t just instill our personal cognition into others, nor can we blindly believe in whatever others say. All in all, human practice is all about “gain, give and mutual interaction”.

First, we build up a system constructed by the three topics below: Project, Us and the World. What we found is that our activities can actually made up a comprehensive interaction (refer to the following picture beside) For example, we “gain” professional suggestions from experts in various fields, which have made us think of repositioning our project; To conduct educating, we “give” what we know to those who are laymen in synthetic biology and sugar controlling. Other examples of give and gain also includes marketing and sponsorships. It is also necessary that we set up mutual pathways for knowledges, which are also known as meetups, collaborations and cross-domain communications. Totaling up all of them, we’ve excellently completed human practice.

To achieve our goal, we consult experts in different areas of study

Expert Consultation

Nephrologist

  • Time

    7/22

  • Location

    Bio-Lab

  • Consultant

    Dr. Wu

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Nepthrologist

Taiwan has the reputation of “the kingdom of Hem dialysis”. Yes, this is apparently not a thing we should be proud of. A huge sum of money is spent on this problem every year. But, you probably don’t know that a huge ratio of these cases are caused by neither drug abuse nor consuming too much salty food. Instead, diabetes is what may leads to kidney diseases. So, we invited Dr. Wu from the division of nephrology to tell us about the negative effects of diabetes. 

 In the interview, he mentioned that due to hyperglycemia, HHNK and DKA, which may cause headache, nausea, or even drive people into coma, is often found in people with diabetes. Furthermore, hyperglycemia can also cause the filtration in the glomerular to accelerate, which leads to its hypertrophy. As the function of kidney is gradually damaged, it can no longer eliminate the deleterious substances from our body. With urea and creatinine surging, a person could eventually end up in renal failure. From above, we can get a conclusion that diabetes is surely a thorny chronic disease that we should be cautious of.

Taiwan has the reputation of “the kingdom of Hem dialysis”. Yes, this is apparently not a thing we should be proud of. A huge sum of money is spent on this problem every year. But, you probably don’t know that a huge ratio of these cases are caused by neither drug abuse nor consuming too much salty food. Instead, diabetes is what may leads to kidney diseases. So, we invited Dr. Wu from the division of nephrology to tell us about the negative effects of diabetes. 

 In the interview, he mentioned that due to hyperglycemia, HHNK and DKA, which may cause headache, nausea, or even drive people into coma, is often found in people with diabetes. Furthermore, hyperglycemia can also cause the filtration in the glomerular to accelerate, which leads to its hypertrophy. As the function of kidney is gradually damaged, it can no longer eliminate the deleterious substances from our body. With urea and creatinine surging, a person could eventually end up in renal failure. From above, we can get a conclusion that diabetes is surely a thorny chronic disease that we should be cautious of.

 

Fitness Coach

  • Time

    7/29

  • Location

    Smooth Fitness Studio

  • Consultant

    Coach Wei Qian-Fu

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Coach

 

Nutritionist

  • Time

    9/11

  • Location

    McDonald's

  • Consultant

    Nutritionist Liu Guan-Ling

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Nutritionist

Motivation : When visiting the fitness coach, he suggested that we also consult a nutritionist, assuming that the answers agreed by different professions may be somewhat different. Based on the recommendations of the fitness coach, we found a nutritionist from Cheng Ching Hospital, hoping to get the answer that is beneficial to our project.

Highlight :
  1. Processed sugar intake accounted for a person's daily calories required 10%
  2. Excessive diet can hurt the body
  3. The amount of calories required for a day minus about 400 calories is the most appropriate calorie needed for dieting
  4. Fat is also one of the main causes of obesity
  5. Note the control of blood sugar

Conclusion :
After this interview, we found that the point of discussion nutritionist and fitness coach stand are similar in many ways : for instance, going on diet should not be excessive, controlling  food and drink is important. These are complementary to what we want to do. This interview also let us adjust our project in order to support the public in pursue of a healthy and better life.

 

Outreach

Science, not only the lab, but into into the public


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The Poll

Time:     9/16

Location:
   Taichung City

Participants:
The public

Walk into the public!

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The Poll

 

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S&T Month

Time:     5/22, 23, 25

Location:
   Lecture Hall & Bio-Lab

Participants:
Mingdao students

iGEM in Campus!

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S&T month

S&T Month is a tech activity hold by Mingdao annually. In the period time of five weeks, teachers and students trained in Physics, Chemical, Earth science, Biology and iGEM lead the other students to have an inside look into the world of science. 2017 Mingdao Team arranged two days of experiment and one day of a lecture. It is because iGEM still remains unfamiliar to them that the registration is quite enthusiastic. Such an optimistic start it is our campaign on iGEM has.  


 The first experiment is plasmid DNA extraction. After explaining the concept of it, we divided them into six groups, and chose a leader from our team members for every group. Most of the students had never used pipette before, which made them tense but eager to try. After all, what we students usually use are always droppers, beakers…and so on. 

  During the experiment day, we showed them our newly-opened-up lab and had them take turn operating at the same time. They all felt it cool and funny to extract the plasmid, and showed more interest in the experiment, electrophoresis, the next day. Not only did they ask questions enthusiastically, but they were more skilled because of the practices the day before.

As for the lecture on the third day, we told them more about what exactly Molecular biology is, and we invited a senior who had engaged in iGEM last year shared his experience and what he learned in iGEM. 

This activity gives middle school students a basic idea of Molecular biology. Moreover, it helps increase iGEM’s popularity as well. It is really killing two birds with one stone. 

 

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Elementary School

Time:     9/22

Location:

Chiao-Jen Elementary School

Participants:
Kids

Sow Seed of Science

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Elementary School Promotion


After S&T Month, we decided to extend our educate range to elementary school students. To let more people know the spirit of iGEM, we went to an elementary school, Chiao-Jen, to teach students there some basic concept of synthetic biology and introduce iGEM to them.

   We prepared a game for them and a bag of candy as reward, making it easier to understand what we want to express. Every team had one million dollars to invest in the genetically modified product. In the meanwhile, we mixed some fundamental idea of the synthetic biology into the game. Then, we explained the relationship between DNA and gene briefly. To our surprise, there was even a little boy asking us our opinion on GMO foods! 

In this activity, we find It is the passionate interaction students react to you that what the most enjoyable moment of teaching is.  It was their passionate participation that made this activity succeed.

 

Monterosso

Drink Sampling

Time:     10/3

Location:

Mingdao High School

Participants:

Mingdao staff and students

Just Drink It!

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Drink Sampling

 


Food and Drug Administration

Time : 9/27

Location : TFDA Office Building

Participant : Section Manager Zhou


Food Industry Research and Development Institute

Time : 9/29

Location : FIRDI Research Building

Participant : Doctor Zhu Wen-Shen

Science that benefits reality

Cross Domain Integration

Forum - The Mingdao Voice

Not limited to laboratory, we make business approach

Enterprise Outreach

Visit to Chung Mei Pharmaceutical Co., Ltd.

Part Name [Number]:

  1. Pcar, synthetic promoter repressed by CRP [BBa_K861171]
  2. RBS + PhlF repressor + terminator [BBa_K1725041]
  3. PhlF repressible promoter + strong RBS + GFP [BBa_K1725001]

Triggering the suicide circuit

In our case of sugar hijacking, ideally, the tiny, living “agents” are supposed to kill themselves in the story to make a happy ending. Therefore, we introduced a glucose responsive elements and a repressor circuit to be connected to suicide genes (lysis and NucA).

Promoter Pcar [BBa_K861171] is a glucose responsive promoter created by WHU-China in 2012. Pcar promoter region was de novo designed with overlapping of CRP and RNA polymerase binding site. The initiation of transcription by RNA polymerase may be hindered by the binding of CRP, which occurs at the formation of cAMP-CRP complex in the low concentration of glucose. In other words, when the amount of glucose is high enough, Pcar would be turned on after the leaving of CPR due to the low concentration of cAMP, and vice versa.

PhlF repressor system contains the repressor PhlF [BBa_K1725041] and the PhlF repressible promoter [BBa_K1725001] created by Glasgow in 2015. PhlF could repress GFP fluorescence intensity by 83-fold according to the study of Glasgow’s work.

Glucose responsive repressor device

We’ve innovated this year a novel glucose responsive repressor system (Pcar-wRBS-PhlF-T-Pr-sRBS-GFP/pSB1C3 [BBa_K2230012]) by connecting these two system and extend the function of them.

Please go to DEMONSTRATION section below to check the function of this device in our experimental results.

  • - REFERENCE -
  • 1.     Amount of colicin release in Escherichia coli is regulated by lysis gene expression of the colicin E2 operon. PLoS One. 2015;10(3):e0119124.
  • 2.     Characterization of a nuclease produced by Staphylococcus aureus. J Biol Chem. 1967;242(5):1016-20.

Conclusion





It took us quite a long time to think about how to respond to the questions above, since last time we had a meetup with NCKU_Tainan, one of their team members had already ask us a pretty tough question similar like these problems that we barely couldn’t answer, “aren’t you guys supporting the world to consume more sugar?” At first, we kind of think that they are right. After all, there’s no denying that these  questions just hit the weak point of our project and present the realistic society problems our product might bring, but after a fierce argument between our teammates, we repositioned our project and have the conclusion in addition below :


 

Indeed. It is actually the way that gets straight to the point the most. However, it’s just like the reason why you prefer Mosquito-repellent lotion to wearing long sleeves and trousers when hanging out with friends in a hot day. You don’t like to sweat and become smelly, so you make the choice. Let’s have another example. If you have motion sickness, the most simple and direct way to solve it is get off the car, but that’s too inconvenient. As a result, you take motion sickness pills instead. The two examples convey the same meaning. Sugar is not merely an essential nutrient in our normal life, but it is also added into snacks、afternoon tea… and so on that people enjoy for relaxation. That is, avoiding eating sugar may probably not be very efficient to reach the goal of losing weight. At this moment, our bacteria come into use. 


But here comes a question. Eating sugar without absorbing it? People will definitely run into sugar’s hug rather than resist the desire.  

  Yes. Thus, instead of the medicines put on the shelf that everyone can purchase, our product will be prescription medicines for assistant purpose. Whether patients can take it to help control their weight or blood sugar depends on doctors’ judgment. The customer base we set as target is mostly those who need to lose weight. By doing so, our product won’t be the permit to the heaven of sugar.   

So we come to the third question. In fact, this situation won’t happen no matter whether our product is prescription medicines for assistant purpose. That is because the sugar nowadays we use is mostly Corn syrup. The growth of corn is all mechanized and at area of large scale of land, which is as they are like apples and oranges when it comes to sucrose farmers exploitation. That’s why most news about farmer exploitation focuses on cocoa beans or coffee beans instead of sucrose.  

There are always two sides to one coin. Everything has its own defect no matter how perfect it is. Facing the fault, what we should do is manage to solve it in preference to leaving it to the unsure factors in the future. Thank The Mingdao Voice for helping us passionately. It is this bilateral communication between science and humanities and social science that makes our project more specific and more mature to be closer to come true.

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