Parts
During the summer, several basic parts were constructed. Some of these were not designed using the Rfc10 assembly standard but, rather, with the Rfc25 in order to construct fusion proteins.
We designed 3 parts for the quorum sensing approach:
- Enoyl-CoA hydratase (Rfc10) - BBa_K2255000
- Acyl-CoA isomerase (Rfc10) - BBa_K2255001
- Thioesterase (Rfc10) - BBa_K2255002
We designed 2 parts to disrupt biofilms:
- Multi-Tag (Rfc25) - BBa_K2255003
- EPS-Depolymerase (Rfc25) - BBa_K2255006
Finally, we designed some basic parts for the phage-like particle approach:
- Xylella fastidiosa constitutive promoter + strong RBS (Rfc10) - BBa_K2255004
- Domain 3 of p3 from M13 (Rfc25) - BBa_K2255005
- Signal sequence of p3 from M13 (Rfc25) - BBa_K2255007
Then a collection of parts to use for the targeting of phage-like particles, each part corresponds to the domaine 1 (D1) and 2 (D2) of a different p3 protein. To test the specificity of our phage-like particle and to target other pathogenic bacteria, we made a collection of targeting parts:
- p3_E.coli (Rfc25) - BBa_K2255008
- p3_N.gonorrhoeae (Rfc25) - BBa_K2255009
- p3_P.aeruginosa (Rfc25) - BBa_K2255010
- p3_R.solanacearum_RSM1 (Rfc25) - BBa_K2255011
- p3_R.solanacearum_RSS1 (Rfc25) - BBa_K2255012
- p3_V.Cholerae_CTXΦ (Rfc25) - BBa_K2255013
- p3_V.Cholerae_fs2 (Rfc25) - BBa_K2255014
- p3_V.Cholerea_VGJΦ (Rfc25) - BBa_K2255015
- p3_X.campestris (Rfc25) - BBa_K2255016
- p3_X.fastidiosa (Rfc25) - BBa_K2255017
- p3_X.fuscans (Rfc25) - BBa_K2255018