Team:Amsterdam/test/thijs/index
We are running out of oil and the climate is changing drastically due to the emission of polluting gases such as CO 2 . But what if CO 2 were a resource, rather than a wasteful pollutant; and might even replace oil? We aspire to create a bio-based economy – one that meets its energy and production demands by leveraging biotechnology [1]. We can genetically engineer bacteria to produce a compound we need. This is what we call a bacterial ‘cell factory’. Meet CYANOFACTORY , a cyanobacterium that stably and efficiently produces fumarate.
OUR GOAL
We use the cyanobacterium Synechocystis PCC6803, a photosynthetic model organism of which the genetic toolbox is rapidly expanding.
Click here for a live feed with our Synechocystis cultivators.
SYNECHOCYSTIS
Fumarate is a multifaceted acid that is used as a plastic precursor, an additive for the food industry and a drug against multiple sclerosis and psoriasis.
Click here to see what we did with Fumarate.
FUMARATE
Biosensor
A biosensor for fumarate is constructed in order to facilitate a high throughput screening of extracellular fumarate. This is essential for low measurement time and costs.
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Transporter
In anticipation of high fumarate production, transport of fumarate out of the cell can be a limiting factor. This mechanism is largely unknown in Synechocystis and will therefore be -guided by bioinformatics- characterized by means of knock-out and over- expression experiments.
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Glyoxylate Shunt
We will incorporate two enzymes (the glyoxylate shunt) in the Synechocystis genome, creating a shortcut in the TCA-cycle. Our modeling results show that if this shunt is only expressed at night, and the pentose phosphate pathway is blocked by knocking out the zwf gene, more fumarate will be produced. This means we will create a unique combination of growth- coupled and inducible production.
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Stability
When an organism is genetically modified to create a product, it causes a decrease in growth rate. This means that a mutated - non- producing - organism will grow faster and take over the population. This problem is tackled by coupling fumarate production to growth. Given that evolution selects heavily on growth rate, we now have a way of naturally selecting for production rate [4]! Knocking out the fumarate degrading reaction in the TCA cycle causes fumarate to be produced in a growth coupled way.
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MODULES
