Collaboration – Mentoring iGEM Team UNIFI 2017
Figure 1: Skype meetings with iGEM UNIFI for a two-way collaboration.
In return for the mentorship, iGEM UNIFI helped us characterizing two BioBricks. To make sure that Escherichia coli is able to take up the unnatural nucleoside triphosphates from the cultivation media we had to introduce a heterologous transporter. One of the BioBricks encodes a complete nucleotide transporter PtNTT2 (BBa_K2201004) originated from the algae Phaeodactylum tricornutum. The second BioBrick is a truncated version missing the N-terminal signal peptide (BBa_K2201001). This N-terminal signal peptide leads to some kind of toxicity in E. coli. Through cultivation experiments we wanted to investigate the extent of the toxicity by comparing the growth of the strain expressing the full version of PtNTT2 to the ones expressing the truncated version.
iGEM UNIFI had the capacity to perform the same cultivation experiment we performed using a microscale bioreactor. This ensured automatic measurements for OD600 values which would decrease errors concerning µmax. This characterization from iGEM UNIFI led to a more accurate estimation of the toxicity of a full length version compared to a truncated version of PtNTT2.
Cultivation performed by iGEM team UNIFI as part of our collaboration
Collaboration - Lokalization Study and Part Exchange with CU Boulder 2017
Figure 2: 3D-Animation of the fluorescence signal of E.coli cells transformed with EutC-tagged FusionRed from CU Boulder. The fluorescence is present in the whole cell.
Figure 3: 3D-Animation of the fluorescence signal of three E.coli cells cotransformed with shell protein EutS and EutC-tagged FusionRed from CU Boulder. The fluorescence is concentrated in the EutS-compartments.
We are very happy that they provided their aaRS to us to expand our toolkit and we hope that our results of the localization study are helpful for their further work.
22.04 - March for Science in Bonn
Figure 4: Daniel Bergen at the March for Science in Bonn at the 22nd April with members of the team Cologne Duesseldorf and other iGEM teams.
13.05, 15.07 - Meetings with Team Cologne Duesseldorf
Figure 5: Impressions of our meet ups with the team Cologne Duesseldorf. Our visit in Dusseldorf left and the meet up in Bielefeld right.
30.06 - German Meet-up in Dresden
Figure 6: Our speaker Chris Whitford at the German meet-up in Dresden, presenting our project to the public for the first time.
06-08.07 - European Meet-up in Delft
Figure 7: Six of our team members at the European meet-up in delft.
Figure 8: Collection of the postcards of iGEM teams from around the world. Our card is the black one in the lower middle.