Team:Bielefeld-CeBiTec/Notebook/Oligonucleotides
Oligonucleotides
| ID | Name | Length | Tm °C | Sequence 5'-> 3' |
|---|---|---|---|---|
| 17aa | tetM_gaF | 48 | 61.0 | ACAATGCTAGCGACAAAGAGGAGAAAGTAATGCGCACCCTGAACATCG |
| 17ab | tetM_gaR | 49 | 60.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTACTACTCACCCGAATGCCCG |
| 17ac | tetM_gaF | 60 | 56.0 | ACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAAATCATAAATATCGGTGTATTAGCTC |
| 17ad | tetM_gaR | 60 | 55.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATGTGATTTTGTTGAACATATAACGAAC |
| 17ae | tetM_gaF | 64 | 56.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAAATTATTAATATTGGAGTTTTAGCTCATG |
| 17af | tetM_gaR | 74 | 55.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTAAGTTATTTTATTGAACATATATCTTACTTTATCTATCC |
| 17ag | pSB1C3-mRFP_gaF | 49 | 68.0 | CTAGTGTAGATCGCGGTCTCATGCGTACTAGTAGCGGCCGCTGCAGTCC |
| 17ah | pSB1C3-mRFP_gaR | 73 | 68.0 | CTCCTCTTTGTCGCTAGCATTGTACCTAGGACTGAGCTAGCCATAAACTCTAGAAGCGGCCGCGAATTCCAGA |
| 17ai | aac2_gaF | 49 | 62.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGACCATCGACCTCCGCC |
| 17aj | aac2_gaR | 48 | 62.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGGTGACGTCGCCGTC |
| 17ak | mRFP_gaF | 68 | 58.0 | CTAGGTACAATGCTAGCGACAAAGAGGAGAAAGTAATGGGCGAGACCGCTTCCTCCGAAGACGTTATC |
| 17al | mRFP_gaR | 65 | 58.0 | TTTTGCCGGACTGCAGCGGCCGCTACTAGTACGCATGAGACCGCGATCTACACTAGCACTATCAG |
| 17am | aadA_gaF | 49 | 60.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAGGGAAGCGGTGATCG |
| 17an | aadA_gaR | 58 | 59.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGCCGACTACCTTGGTGATCTCG |
| 17ao | ermc_gaF | 62 | 57.0 | ACAATGCTAGCGACAAAGAGGAGAAAGTAATGAACGAGAAAAATATAAAACACAGTCAAAAC |
| 17ap | ermc_gaR | 78 | 58.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTACTTATTAAATAATTTATAGCTATTGAAAAGAGATAAGAATTGTTC |
| 17aq | msrA_gaF | 67 | 58.0 | ACAATGCTAGCGACAAAGAGGAGAAAGTAATGGAACAATATACAATTAAATTTAACCAAATCAATCA |
| 17ar | msrA_gaR | 67 | 58.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAAGTTATATCATGAATAGATTGTCCTGTTAATTCC |
| 17as | mef_gaF | 62 | 58.0 | ACAATGCTAGCGACAAAGAGGAGAAAGTAATGGAAAAATACAACAATTGGAAACTTAAGTTT |
| 17at | mef_gaR | 71 | 58.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTTAAATCTAATTTTCTAATCTCATTTATCATTGGGC |
| 17au | ermX_gaF | 48 | 58.0 | ACAATGCTAGCGACAAAGAGGAGAAAGTAATGTCTGCATACGGACACG |
| 17av | ermX_gaR | 51 | 58.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCACCTGGAAGAGATCGATCC |
| 17aw | carB4_gaF | 54 | 60.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAGTTGCTGTTAGTGTTCTCG |
| 17ax | carB4_gar | 53 | 59.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAACGGCTTTGCGAACTATAAA |
| 17ay | tetX_gaF | 47 | 61.0 | TGCTAGCGACAAAGAGGAGAAAGTAATGAGACCTCGTATCGCTGTGG |
| 17az | tetX_gaR | 43 | 62.0 | CGGACTGCAGCGGCCGCTACTAGTATCAAGCAGCAGGGCAGCG |
| 17ba | addA1_gaF | 55 | 59.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGCCGACTACCTTGGTGATC |
| 17bb | addA1_gaR | 49 | 60.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAGGGAAGCGGTGATCG |
| 17bc | addA1_gaF | 49 | 59.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAGGGAAGCGGTGATCG |
| 17bd | addA1_gaR | 53 | 58.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGCCGACTACCTTGGTGA |
| 17be | aad9_gaF | 52 | 57.0 | GCTAGCGACAAAGAGGAGAAAGTAATGAGGAGGATATATTTGAATACATACG |
| 17bf | aad9_gaR | 73 | 54.0 | CGGACTGCAGCGGCCGCTACTAGTATTATAATTTTTTTAATCTGTTATTTAAATAGTTTATAGTTAAATTTAC |
| 17bg | aac3_gaF | 53 | 61.8 | TACAATGCTAGCGACAAAGAGGAGAAAGTAGTGCAATACGAATGGCGAAAAGC |
| 17bh | aac3_gaR | 50 | 62.6 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGCCAATCGACTGGCGAG |
| 17bi | tetA_gaF | 55 | 59.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGAAATCTAACAATGCGCTCATCG |
| 17bj | tetA_gaR | 48 | 60.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTAGGTCGAGGTGGCCCG |
| 17bk | pbp5_gaF | 57 | 59.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGGAACGAAGCAATCGTAATAAAAAG |
| 17bl | pbp5_gaR | 65 | 59.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTAATGGTTGCTTCTAAGTAATCAATTAATG |
| 17bm | hyg_gaF | 61 | 66.7 | TACAATGCTAGCGACAAAGAGGAGAAAGTAGTGACACAAGAATCCCTGTTACTTCTCGACC |
| 17bn | hyg_gaR | 46 | 67.9 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGGCGCCGGGGGCG |
| 17bo | arpA_gaR | 51 | 61.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGCCTTTCGGTGTTTCCAC |
| 17bp | arpA_gaF | 50 | 61.0 | TACAATGCTAGCGACAAAGAGGAGAAAGTAATGCAATTCAAGCCAGCCGT |
| 17bq | ecoRI(1)35 | 35 | 63.0 | GAAGGTCGCCTGGAGTTCTCCGAAGTCGCGGTGGA |
| 17br | ecoRI(1)re | 35 | 63.0 | GCGACTTCGGAGAACTCCAGGCGACCTTCCTGCTT |
| 17bs | ecoRI(2)fw | 36 | 64.0 | CATTCTGATCGTGGAGTTCGCCAAGGAGCTGCACGA |
| 17bt | ecoRI(2)re | 40 | 64.0 | AGCTCCTTGGCGAACTCCACGATCAGAATGGCGTTTTTCG |
| 17bu | PlacUV5_ga1F | 55 | 56.0 | TTTACACTTTATGCTTCCGGCTCGTATAATGGACAAAGAGGAGAAAGTAATGGGC |
| 17bv | PlacUV5_ga1R | 19 | 58.0 | CAGCGGCCGCTACTAGTAC |
| 17bw | PlacUV5_ga2F | 19 | 58.0 | GTACTAGTAGCGGCCGCTG |
| 17bx | PlacUV5_ga2R | 48 | 58.0 | CATTATACGAGCCGGAAGCATAAAGTGTAAACTCTAGAAGCGGCCGCG |
| 17by | PtNTT2_gaF | 47 | 60.0 | GCTCGTATAATGGACAAAGAGGAGAAAGTAATGCGCCCGTATCCGAC |
| 17bz | PtNTT2_gaR | 58 | 57.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTTTCTTTGTTGGTTTTCGCTTCC |
| 17ca | LFS_ovexPCR_F | 51 | 58.0 | TCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCC |
| 17cb | LFS_ovexPCR_R | 50 | 57.0 | ACTGAGCCTTTCGTTTTATTTGATGCCTGGAATGCCTCCCGGATAAAACA |
| 17cc | Term_ovexPCR_F | 53 | 57.0 | TTTTCAGATGTGTTTTATCCGGGAGGCATTCCAGGCATCAAATAAAACGAAAG |
| 17cd | Term_ovexPCR_R | 50 | 57.0 | GAGCGCTCACAATTCTCACGCTTTCCAGTCTATAAACGCAGAAAGGCCCA |
| 17ce | lacO_ovexPCR_F | 41 | 58.0 | TGGGCCTTTCTGCGTTTATAGACTGGAAAGCGTGAGAATTG |
| 17cf | lacO_ovexPCR_R | 57 | 57.0 | ATCTAAGCCTATTGAGTATTTCTTATCCATCTAGTATTCCTGTGTGAAATTGTTATC |
| 17cg | cas9_ovexPCR_F | 58 | 57.0 | GCGGATAACAATTTCACACAGGAATACTAGATGGATAAGAAATACTCAATAGGCTTAG |
| 17ch | cas9_ovexPCR_R | 80 | 57.0 | ATGGTAATGTTGCTCATATCAGTATCTCATTTTACGGCGGTCGCCTTGACCGCCGACACCCTCAGTCACCTCCTAGCTGA |
| 17ci | RFS_ovexPCR_F | 80 | 57.0 | GAGTCAGCTAGGAGGTGACTGAGGGTGTCGGCGGTCAAGGCGACCGCCGTAAAATGAGATACTGATATGAGCAACATTAC |
| 17cj | RFS_ovexPCR_R | 59 | 58.0 | TTAGAACAGGAATGGATTTTCTTCTACACACAATAAATGTAATGCGTTATATCTTCCTG |
| 17ck | Sequ_ftsz_R | 23 | 53.0 | GTAAGTTCCATTGGTTCAAACAT |
| 17cl | Sequ_ftsz_F | 21 | 55.0 | CTGCGTAAGCAAGCTGATTAA |
| 17cm | Sequ_tet_R | 21 | 53.0 | GAGCGCATTGTTAGATTTCAT |
| 17cn | Sequ_tet_F | 20 | 65.0 | GCCGGGCCACCTCGACCTAA |
| 17co | Sequ_kan_R | 21 | 50.0 | CAATCCATCTTGTTCAATCAT |
| 17cp | Sequ_kan_F | 21 | 53.0 | CTTCTTGACGAGTTCTTCTGA |
| 17cq | codA_flanke1_delF | 46 | 59.0 | TGAGTTAGCTCACTCATTAGGCACCCATTAAAAGCGGTCGTTCCCG |
| 17cr | codA_flanke1_delR | 27 | 60.0 | TTATTCGACACTGTTAGCCTCCACATG |
| 17cs | codA_flanke2_delF | 44 | 60.0 | TGTGGAGGCTAACAGTGTCGAATAAACGACTGGGTTACAGCGAG |
| 17ct | codA_flanke2_delR | 45 | 58.0 | GACTGGCTTTCTACGTGTTCCGCTTCGGAATTTGCCACCAGAGAG |
| 17cu | sgRNA_LKA01_for | 24 | 0.0 | ACGCTATTGTTCATAATAGAAGAG |
| 17cv | sgRNA_LKA01_rev | 24 | 0.0 | AAACCTCTTCTATTATGAACAATA |
| 17cw | PtNTT2_assembly_gaF | 20 | 60.0 | GCGGCGGATAACGAACAGAG |
| 17cx | PtNTT2_assembly_gaR | 23 | 58.0 | TAATGCTCTGTTCGTTATCCGCC |
| 17cy | Sequ_MCS_F | 20 | 54.0 | GCGAATTGGGTACACTTACC |
| 17cz | Sequ_MCS_R | 21 | 54.0 | CACTAAAGGGAACAAAAGCTG |
| 17da | Sequ_pBK1_F | 20 | 64.0 | CACCTGACGCGCCCTGTAGC |
| 17db | Sequ_pBK2_F | 24 | 54.0 | GCATTCATTTTATGTTTCAGGTTC |
| 17dc | Sequ_pBK3_F | 21 | 54.0 | CAGCTTTTGTTCCCTTTAGTG |
| 17dd | Sequ_pBK4_F | 21 | 54.0 | CAGAAGAACTCGTCAAGAAGG |
| 17de | Sequ_54_F | 22 | 59.0 | GAACGCTCTCCTGAGTAGGACA |
| 17df | Sequ_55_F | 21 | 66.0 | GGCGCAGCGGTCGGACTGAAC |
| 17dg | Sequ_56_F | 21 | 64.0 | CTGCCCTGCTGAACTACCGCC |
| 17dh | Sequ_58_R | 21 | 60.0 | GTAATCGCTGAATTCCCGGCG |
| 17di | Prefix_fwd | 19 | 59.0 | TTTCTGGAATTCGCGGCCG |
| 17dj | Suffix_rev | 17 | 60.0 | TTTGCCGGACTGCAGCG |
| 17dk | KanMod-Fwd-1 | 57 | 61.0 | CATAAACAGTAATACAAGGGGTGTTATGTAGAGCCATATTCAACGGGAAACGTCTTG |
| 17dl | KanMod-Fwd-2 | 60 | 61.0 | CATAAACAGTAATACAAGGGGTGTTATGTAGTAGAGCCATATTCAACGGGAAACGTCTTG |
| 17dm | KanMod-Fwd-3 | 63 | 60.0 | CATAAACAGTAATACAAGGGGTGTTATGTAGTAGTAGAGCCATATTCAACGGGAAACGTCTTG |
| 17dn | KanMod-Rev | 32 | 61.0 | AACACCCCTTGTATTACTGTTTATGTAAGCAG |
| 17do | KanMod-Split-Fwd | 33 | 58.0 | GTAGTGATCTTATTTCATTATGGTGAAAGTTGG |
| 17dp | KanMod-Split-Rev | 33 | 58.0 | CCAACTTTCACCATAATGAAATAAGATCACTAC |
| 17dq | KanMod-19ML | 24 | 55.0 | GCTTAAACTCCAACTTGGACGCTG |
| 17dr | LacO_GA_For | 52 | 61.0 | ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAG |
| 17ds | LacO_GA_Rev | 49 | 61.0 | GGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAG |
| 17dt | VR | 20 | 56.0 | GCTCACTCAAAGGCGGTAAT |
| 17du | VF2 | 20 | 57.0 | TGCCACCTGACGTCTAAGAA |
| 17dv | pK18-wo-ori_fwd | 14 | 59.0 | ACGCCAGCAACGCG |
| 17dw | pK18-wo-ori_rev | 29 | 60.0 | GAAGATCCTTTGATCTTTTCTACGGGGTC |
| 17dx | pSC101-replicon-gaF | 45 | 56.0 | CCGTAGAAAAGATCAAAGGATCTTCGAGTTATACACAGGGCTGGG |
| 17dy | pSC101-replicon_gaR | 49 | 57.0 | CGTAAAAAGGCCGCGTTGCTGGCGTTCAGATCCTTCCGTATTTAGCCAG |
| 17dz | RubiscoTAG2_fw | 50 | 68.0 | ATGCAGGATTACAAGCAAAGCCTCAAATATGAGACTTTCTCTTATCTTCC |
| 17ea | RubiscoTAG2_rv | 60 | 68.0 | TGAGGCTTTGCTTGTAATCCTGCATCTAAGCCATGATATCTTTCTCCTCTTTCTCTAGAA |
| 17eb | RubiscoTAG111_fw | 52 | 68.0 | AAAGAGGAGAAATACTAGATGGCAGTTAAGAAATATAGCGCGGGTGTCAAAG |
| 17ec | RubiscoTAG111_rev | 57 | 68.0 | CTGCCATCTAGTATTTCTCCTCTTTGATATCTTACTAGTTGCCGCGGTAGACCACGA |
| 17ed | RubisoTAG474_fw | 45 | 68.0 | TACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTC |
| 17ee | RubisoTAG474_rev | 60 | 68.0 | CGGACTGCAGCGGCCGCTACTAGTAGATATCTTACTAACGGTTTTGTGTGTCCAACTTAT |
| 17ef | RubisomRFP_fw | 42 | 64.0 | CTTCGTGGTCTACCGCGGCAACTACTAGAGAATGGCTTCCTC |
| 17eg | RubiscomRFP_rev | 56 | 63.0 | GCGCTATATTTCTTAACTGCCATCTAGTATTTCTCCTCTTTGCGATCTACACTAGC |
| 17eh | mRFPRubisco_fw | 36 | 54.0 | GATAGTGCTAGTGTAGATCGCAAAGAGGAGAAATAC |
| 17ei | mRFPRubisco_rev | 33 | 55.0 | GTCTTCGGAGGAAGCCATTCTCTAGTAGTTGCC |
| 17ej | Sequ_pBAD_fw | 18 | 62.0 | GTTGCGTCGCGGTGCATG |
| 17ek | Sequ-pBAD_rev | 20 | 58.0 | CAATCTCACCATTCGCGGTC |
| 17el | VR | 20 | 56.0 | ATTACCGCCTTTGAGTGAGC |
| 17em | LFS_GA_F | 64 | 65.0 | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCATTGCCAGAGTGAAAATGCCTCAGAGCT |
| 17en | LFS_GA_R | 74 | 65.0 | CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAAATGCCTCCCGGATAAAACACATCTGAAAATTCATATGT |
| 17eo | BB_LFS_F | 56 | 69.0 | TTTTCAGATGTGTTTTATCCGGGAGGCATTTACTAGTAGCGGCCGCTGCAGTCCGG |
| 17ep | BB_LFS_R | 64 | 68.0 | TAGCTCTGAGGCATTTTCACTCTGGCAATGCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
| 17eq | Term_GA_F | 57 | 68.0 | ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCCAGG |
| 17er | Term_GA_R | 67 | 69.0 | GGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGG |
| 17es | BB_Term_F | 56 | 69.0 | CACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGG |
| 17et | BB_Term_R | 64 | 68.0 | ACTGAGCCTTTCGTTTTATTTGATGCCTGGCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
| 17eu | lacO_GA_F2 | 62 | 68.0 | ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGGACTGGAAAG |
| 17ev | lacO_GA_R2 | 67 | 69.0 | GGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTCCTGTGTGAAATTG |
| 17ew | BB_lacO_F | 56 | 69.0 | TTGTGAGCGGATAACAATTTCACACAGGAATACTAGTAGCGGCCGCTGCAGTCCGG |
| 17ex | BB_lacO_R | 64 | 68.0 | GAGCGCTCACAATTCTCACGCTTTCCAGTCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
| 17ey | Cas9_GA_F | 69 | 58.0 | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGATAAGAAATACTCAATAGGCTTAGATATCG |
| 17ez | Cas9_GA_R | 80 | 58.0 | GCCGGACTGCAGCGGCCGCTACTAGTATTTTACGGCGGTCGCCTTGACCGCCGACACCCTCAGTCACCTCCTAGCTGACT |
| 17fa | BB_Cas9_F | 80 | 69.0 | GAGTCAGCTAGGAGGTGACTGAGGGTGTCGGCGGTCAAGGCGACCGCCGTAAAATACTAGTAGCGGCCGCTGCAGTCCGG |
| 17fb | BB_Cas9_R | 64 | 68.0 | ATCTAAGCCTATTGAGTATTTCTTATCCATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
| 17fc | RFS_GA_F | 74 | 62.0 | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTGAGATACTGATATGAGCAACATTACCATTTATCACAAC |
| 17fd | RFS_GA_R | 80 | 62.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTACAATAAATGTAATGCGTTATATCTTCCTGATATAATTACCTTTGATTTCC |
| 17fe | BB_RFS_F | 56 | 69.0 | TCAGGAAGATATAACGCATTACATTTATTGTACTAGTAGCGGCCGCTGCAGTCCGG |
| 17ff | BB_RFS_R | 64 | 68.0 | AATGGTAATGTTGCTCATATCAGTATCTCACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
| 17fg | PtNTT2_trunc_gaF | 45 | 60.0 | TCGTATAATGGACAAAGAGGAGAAAGTAATGGCGGCAGCACCGTG |
| 17fh | aaRS_pC3_fw | 37 | 60.0 | CGGCCGCTTCTAGAGGCACCTGAAGTCAGCCCCATAC |
| 17fi | aaRS_RuTAG_rev | 40 | 60.0 | TATCTTTCTCCTCTTTGTGCAAGATTCCGAATACCGCAAG |
| 17fj | RuTAG_aaRS_fw | 38 | 54.0 | TTCGGAATCTTGCACAAAGAGGAGAAAGATATCATGGC |
| 17fk | RuTAG474_pC3_rv | 40 | 54.0 | CGGCCGCTACTAGTAGATATCTTACTAACGGTTTTGTGTG |
| 17fl | pC3_RuTAG474_fw | 45 | 70.0 | CGTTAGTAAGATATCTACTAGTAGCGGCCGCTGCAGTCCGGCAAA |
| 17fm | pC3_aaRS_rev | 55 | 70.0 | GCTGACTTCAGGTGCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGCG |
| 17fn | pC3_RuTAG_fw | 45 | 70.0 | CACACAAAACCGTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAA |
| 17fo | Sequ_pBad3 | 20 | 54.0 | GCACCACCCGGATGCCTATC |
| 17fp | Sequ_pBad4 | 21 | 54.0 | GTCCGCACCGCTGCCGGTAGC |
| 17fq | RuTAG_pC3_rv | 37 | 54.0 | TTGGACACACAAAACCGTTAATACTAGTAGCGGCCGC |
| 17fr | pSB1C3_splitF | 20 | 59.0 | GTCTGACAGCTCGAGGCTTG |
| 17fs | pSB1C3_splitR | 20 | 59.0 | CAAGCCTCGAGCTGTCAGAC |
| 17ft | PlacUV5_ga2R_v2 | 56 | 54.0 | TCTTTGTCCATTATACGAGCCGGAAGCATAAAGTGTAAACTCTAGAAGCGGCCGCG |
| 17fu | PlacUV5_ga2F_v2 | 18 | 57.0 | TACTAGTAGCGGCCGCTG |
| 17fv | pRedET_SeqF_1 | 20 | 57.0 | AGGCGTAGCAGAAACTAACG |
| 17fw | pRedET_SeqF_2 | 20 | 56.0 | CACACAAAGGTCTAGCGGAA |
| 17fx | pRedET_SeqR_1 | 18 | 59.0 | GACGCAGTGACGGCAATG |
| 17fy | pRedET_SeqR_2 | 20 | 58.0 | ATTTCAGCGACCTGTTTGGG |
| 17fz | pK18msb_ga1 | 20 | 58.0 | AAGCGGAACACGTAGAAAGC |
| 17ga | pK18msb_ga2 | 22 | 57.0 | GGTGCCTAATGAGTGAGCTAAC |
| 17gb | K1172904fw_gb1 | 51 | 58.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGCAGCACAGGTTATCAAC |
| 17gc | K1172904re_gb2 | 60 | 57.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATCTGATTTTTGTAAAGGTCTGATAATG |
| 17gd | psB1A3fw_gb3 | 51 | 64.0 | CATTATCAGACCTTTACAAAAATCAGATAATACTAGTAGCGGCCGCTGCAG |
| 17ge | psB1A3re_gb4 | 51 | 63.0 | GTCAAACGTGTTGATAACCTGTGCTGCCATCTCTAGAAGCGGCCGCGAATT |
| 17gf | Glu107_fw | 63 | 67.0 | CTGAAAGCGAAATACGTCTATGGCAGCNNKGAACAGCTGGACAAAGACTACACCCTGAACGTG |
| 17gg | Glu107_rev | 64 | 67.0 | GCTGCCATAGACGTATTTCGCTTTCAGGCCCATCGCTTCGAACACTTTCTTGTTGTAGTCGCCG |
| 17gh | Asp158_Ile159_Leu162_fw | 73 | 67.0 | GAAGTGATCTATCCGATCATGCAGGTGAACNNKNNKCACTATNNKGGCGTAGATGTAGCAGTTGGTGGCATGG |
| 17gi | Asp158_Ile159_Leu162_rev | 59 | 67.0 | GTTCACCTGCATGATCGGATAGATCACTTCCGCCACTTTCGGGTTTTCATCTTCGCGGG |
| 17gj | pSB1C3_lin_fwd | 19 | 59.0 | TACTAGTAGCGGCCGCTGC |
| 17gk | GFPBB_fwd | 51 | 58.0 | CTGGAATTCGCGGCCGCTTCTAGAGATGCGTAAAGGAGAAGAACTTTTCAC |
| 17gl | pSB1C3_lin_rev | 17 | 61.0 | CTCTAGAAGCGGCCGCG |
| 17gm | GFPLink1_rev | 64 | 58.0 | CCCACCACTGCCTCCACTACCTCCAGAGCCCCCTTTGTATAGTTCATCCATGCCATGTGTAATC |
| 17gn | GFPLink2_rev | 64 | 58.0 | CCCACCACTGCCTCCCTAACCTCCAGAGCCCCCTTTGTATAGTTCATCCATGCCATGTGTAATC |
| 17go | Link1Step_fwd | 54 | 61.0 | GGGGGCTCTGGAGGTAGTGGAGGCAGTGGTGGGGCTGAAGCTGGTATCACCGGC |
| 17gp | Link2Step_fwd | 54 | 61.0 | GGGGGCTCTGGAGGTTAGGGAGGCAGTGGTGGGGCTGAAGCTGGTATCACCGGC |
| 17gq | StrepBB_rev | 45 | 61.0 | CTGCAGCGGCCGCTACTAGTATTAGGAAGCAGCGGACGGTTTAAC |
| 17gr | sup35_Histag_fw | 66 | 70.0 | CATCATCACCATCACCACCATCATCACCATTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAA |
| 17gs | sup35_Histag_rev | 67 | 69.0 | GATGGTGGTGATGGTGATGATGAACTTCGTCGTCAACTTCTTCTTCCTGTTCTTTGATCAGAGCGTC |
| 17gt | pSB1C3_sup35_fw | 51 | 70.0 | GAAGTTGACGACGAAGTTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAAA |
| 17gu | pSB1C3_sup35_rv | 56 | 70.0 | CCTGGTTAGAGTCAGACATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTA |
| 17gv | T7RNAP_fw | 59 | 64.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGAACACGATTAACATCGCTAAGAACGA |
| 17gw | T7RNAP_re | 50 | 64.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTACGCGAACGCGAAGTCCG |
| 17gx | psB1C3_T7P_fw | 50 | 63.0 | ATCTTAGAGTCGGACTTCGCGTTCGCGTAATACTAGTAGCGGCCGCTGCA |
| 17gy | psB1C3_T7P_re | 50 | 63.0 | GTCGTTCTTAGCGATGTTAATCGTGTTCATCTCTAGAAGCGGCCGCGAAT |
| 17gz | PtNTT2_seq_1 | 20 | 59.0 | GGCAACCAGGAAGTGACCAA |
| 17ha | PtNTT2_seq_2 | 20 | 60.0 | CACATCAATCGCCGCTTTGC |
| 17hb | PromRBS-Lin-fwd | 25 | 65.0 | TACTAGTAGCGGCCGCTGCAGTCCG |
| 17hc | KanR-Prä-fwd | 54 | 61.0 | ATGCTAGCTACTAGAGTCACACAGGAAAGATGAGCCATATTCAACGGGAAACGT |
| 17hd | PromRBS-Lin-rev | 23 | 57.0 | CTTTCCTGTGTGACTCTAGTAGC |
| 17he | KanMod-19ML-fwd | 43 | 65.0 | TCAGCATCCAAGTTGGAATTTAATCGCGGCCTGGAGCAAGACG |
| 17hf | KanMod-19ML-rev | 43 | 53.0 | GCGATTAAATTCCAACTTGGATGCTGATTTATATGGGTATAAA |
| 17hg | Suff-KanR-rev | 52 | 59.0 | TGCAGCGGCCGCTACTAGTAATTAGAAAAACTCATCGAGCATCAAATGAAAC |
| 17hh | neuKan-mod-TAG1 | 49 | 59.0 | ACTAGAGTCACACAGGAAAGATGTAGAGCCATATTCAACGGGAAACGTC |
| 17hi | neuKan-mod-TAG2 | 52 | 59.0 | ACTAGAGTCACACAGGAAAGATGTAGTAGAGCCATATTCAACGGGAAACGTC |
| 17hj | neuKan-mod-TAG3 | 55 | 59.0 | ACTAGAGTCACACAGGAAAGATGTAGTAGTAGAGCCATATTCAACGGGAAACGTC |
| 17hk | ONBY-out-rev | 26 | 54.0 | ATGAATCTCCAAGTATATAACGACTG |
| 17hl | ONBY-out-fwd | 27 | 63.0 | TAAGATCTGGATCCTCTACGCCGGACG |
| 17hm | NPA/ONBY-switch-fwd | 46 | 59.0 | TTATATACTTGGAGATTCATATGGACGAGTTTGAGATGATTAAGCG |
| 17hn | ONBY/NPA-switch-rev | 47 | 64.0 | TCCGGCGTAGAGGATCCAGATCTTACAGGCGTTTGCGGATTGGTTCC |
| 17ho | pK18msb_seq_1 | 20 | 55.0 | TAGCTATCGCCATGTAAGCC |
| 17hp | pK18msb_seq_2 | 20 | 62.0 | AGCGGAAGAGCGCCCAATAC |
| 17hq | aaRS_pSB1C3_fw | 50 | 58.0 | CGGCCGCTTCTAGAGGATTATCAATTTTAAAAAACTAACAGTTGTCAGCC |
| 17hr | aaRS_pSB1C3_rev | 39 | 58.0 | GCAGCGGCCGCTACTAGTACTCGAGCATGCAAAAAAGCC |
| 17hs | pSB1C3_aaRS_fw | 44 | 65.0 | GCTTTTTTGCATGCTCGAGTACTAGTAGCGGCCGCTGCAGTCCG |
| 17ht | pSB1C3_aaRS_rev | 50 | 65.0 | GTTTTTTAAAATTGATAATCCTCTAGAAGCGGCCGCGAATTCCAGAAATC |
| 17hu | sgRNA_romA_1_fw | 40 | 68.0 | CGTCAGTCCAGATACCTTTGATGTCCTGATTGGAAGGACC |
| 17hv | sgRNA_romA_1_rv | 40 | 68.0 | ACTCCTCCTCTCAAGGACTATAGAAAAAAAGTCCCGCTGC |
| 17hw | sgRNA_romG_2_fw | 40 | 68.0 | GCAGCGGGACTTTTTTTCTATAGTCCTTGAGAGGAGGAGT |
| 17hx | sgRNA_romG_2_rv | 40 | 69.0 | GCTCACAGGACACCTGGTATACGTATTGATCTCACGAGGC |
| 17hy | sgRNA_romd_3_fw | 40 | 69.0 | GCCTCGTGAGATCAATACGTATACCAGGTGTCCTGTGAGC |
| 17hz | sgRNA_romd_3_rv | 40 | 67.0 | GATTATCGGGCAAATACCTTCTCATACGTGACGGATTCCC |
| 17ia | sgRNA_romT_4_fw | 40 | 67.0 | GGGAATCCGTCACGTATGAGAAGGTATTTGCCCGATAATC |
| 17ib | sgRNA_romT_4_rv | 40 | 69.0 | ATACGTTCCCTTTAATTGCCTGCCGGAGTTAAGGACGTTC |
| 17ic | sgRNA_romC_5_fw | 40 | 69.0 | GAACGTCCTTAACTCCGGCAGGCAATTAAAGGGAACGTAT |
| 17id | sgRNA_romC_5_rv | 40 | 68.0 | GCTAGTATTACTCTGATCTGATGTTTGCAGTCGCTGCTGG |
| 17ie | Prefix_rev | 17 | 59.0 | CTCTAGAAGCGGCCGCG |
| 17if | pSC101-ori-part_gaF | 45 | 57.0 | CTGGAATTCGCGGCCGCTTCTAGAGGAGTTATACACAGGGCTGGG |
| 17ig | pSC101-ori-part_gaR | 49 | 58.0 | CGGACTGCAGCGGCCGCTACTAGTATCAGATCCTTCCGTATTTAGCCAG |
| 17ih | sgRNAs_Rom_gaF | 66 | 62.0 | GGCCTTTCTGCGTTTATATACTAGAAGCGGCCGCTGCACGTCAGTCCAGATACCTTTGATGTCCTG |
| 17ii | sgRNAs_Rom_gaR | 78 | 63.0 | AACTAACTAACCCTTAGTGACTCCTGCAGCGGCCGCTACTAGTAGCTAGTATTACTCTGATCTGATGTTTGCAGTCGC |
| 17ij | sgRNA_Rom_BB_gF | 32 | 62.0 | GCTGCAGGAGTCACTAAGGGTTAGTTAGTTAG |
| 17ik | sgRNA_Rom_BB_gR | 32 | 63.0 | CCGCTTCTAGTATATAAACGCAGAAAGGCCCA |
| 17il | SeqCas1 | 20 | 55.0 | ATTTGGCCTTAGCGCATATG |
| 17im | SeqCas2 | 20 | 58.0 | GGCTCCCCTATCAGCTTCAA |
| 17in | SeqCas3 | 21 | 56.0 | TCGATAAAGGTGCTTCAGCTC |
| 17io | SeqCas4 | 20 | 59.0 | GGTTGGGGACGTTTGTCTCG |
| 17ip | SeqCas5 | 22 | 56.0 | GGAAGAGACATGTATGTGGACC |
| 17iq | SeqCas6 | 20 | 60.0 | GCCGTCGTTGGAACTGCTTT |
| 17ir | SeqCas7 | 21 | 56.0 | CAGTCCTAGTGGTTGCTAAGG |
| 17is | SeqCas8 | 20 | 61.0 | AATCTTGGAGCTCCCGCTGC |
| 17it | Seq_LFS | 20 | 57.0 | CGTCCTGATTCAGACCTCCT |
| 17iu | Seq_B0015 | 20 | 58.0 | GGCTCAGTCGAAAGACTGGG |
| 17iv | RuBisCo_RFP_fw | 56 | 64.0 | GCTAGTGTAGATCGCAAAGAGGAGAAATACTAGATGGCAGTTAAGAAATATAGCGC |
| 17iw | Rubisco_RFP_rev | 37 | 64.0 | AGCCATTCTCTAGTAGTTGCCGCGGTAGACCACGAAG |
| 17ix | RFP_RuBisCo_rev | 49 | 64.0 | GTATTTCTCCTCTTTGCGATCTACACTAGCACTATCAGCGTTATTAAGC |
| 17iy | RFP_RuBisCo_fw | 43 | 64.0 | GGCAACTACTAGAGAATGGCTTCCTCCGAAGACGTTATCAAAG |
| 17iz | ori_pSB1C3_fw_66_3 | 39 | 66.0 | CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG |
| 17ja | pSB1C3_ori_rev_66_2 | 33 | 66.0 | CGGAGCCTATGGAAAAACGCCAGCAACGCGGCC |
| 17jb | pSB1C3_ori_fw_65_4 | 54 | 65.0 | CAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGC |
| 17jc | ori_pSB1C3_rev_65_3 | 57 | 65.0 | GTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGC |
| 17jd | J23100_gaF | 43 | 65.0 | CTTAGTGACTCGAATTCGCGGCCGCTTCTAGAGTTGACGGCTA |
| 17je | J23100_gaR | 56 | 65.0 | TAACTAACTAACCCTTAGTGACTCCTGCAGCGGCCGCTACTAGTATATAAACGCAG |
| 17jf | sgRNA_BB_gaF | 56 | 65.0 | CTGCAAACATCAGATCAGAGTAATACTAGCTACTAGTAGCGGCCGCTGCAGGAGTC |
| 17jg | sgRNA_BB_gaR | 62 | 65.0 | ATCAGGACATCAAAGGTATCTGGACTGACGTATAAACGCAGAAAGGCCCACCCGAAGGTGAG |
| 17jh | retBB_Rom_gaR | 38 | 61.0 | CTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGC |
| 17ji | retBB_Rom_gaF | 44 | 60.0 | ATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCTTCCTC |
| 17jj | pSB1C3_aaRS(oT)_fw_66 | 55 | 65.0 | ATTTTAGAGCCAATTAGAAAGAGATTATAATACTAGTAGCGGCCGCTGCAGTCCG |
| 17jk | aaRS(oT)_pSB1C3_rev_58 | 71 | 58.0 | CCGGACTGCAGCGGCCGCTACTAGTATTATAATCTCTTTCTAATTGGCTCTAAAATCTTTATAAGTTCTTC |
| 17jl | ori_pSB1C3_fw_65 | 56 | 65.0 | TAAAAAGGCCGCGTTGCTGGCGTTGTGACCAAACAGGAAAAAACCGCCCTTAACAT |
| 17jm | neu_pSB1C3_ori_fw_58 | 51 | 58.0 | TACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGG |
| 17jn | pSB1C3_ori_rev_65 | 40 | 65.0 | GCGGTTTTTTCCTGTTTGGTCACAACGCCAGCAACGCGGC |
| 17jo | KanR-mod-fwd (80) | 80 | 72.0 | ACTAGAGTCACACAGGAAAGATGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCGCTTGGGTTGAGAGGCTATTC |
| 17jp | KanR-mod-rev (80) | 80 | 72.0 | CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGAAGAACTCGTCAAGAAGGCGATAGAAGGCGATGCGC |
| 17jq | KanR-TAG1-fwd (73) | 73 | 71.0 | AATGCTAGCTACTAGAGTCACACAGGAAAGATGTAGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCG |
| 17jr | KanR-TAG2-fwd (76) | 76 | 71.0 | AATGCTAGCTACTAGAGTCACACAGGAAAGATGTAGTAGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCG |
| 17js | KanR-TAG3-fwd (79) | 79 | 71.0 | AATGCTAGCTACTAGAGTCACACAGGAAAGATGTAGTAGTAGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCCG |
| 17jt | KanR-mod-fwd-short (59) | 59 | 71.0 | ACTAGAGTCACACAGGAAAGATGATTGAACAAGATGGACTACACGCAGGTTCTCCGGCC |
| 17ju | pSB1C3_sup_fw | 70 | 70.0 | GATCAAAGAACAGGAAGAAGAAGTTGACGACGAAGTTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAAA |
| 17jv | pSB1C3_sup_rev | 68 | 68.0 | GCTGGTTGTTACCCTGGTTAGAGTCAGACATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTA |
| 17jw | sup35_121CUA_fw | 50 | 61.0 | CCAGGCTGGTTTCCAGCCGCAGCTACAGGGTATGTCTCTGAACGACTTCC |
| 17jx | sup35_121CUA_rv | 55 | 61.0 | TAGCTGCGGCTGGAAACCAGCCTGGTAACCTTGCAGGTTGTTGTTGTAGTTGAAG |
| 17jy | AcF_CUA_fw | 54 | 67.0 | CGGACTTAGAATCCGCATGGCAGGGGTTCAAATCCCCTCCGCCGGACCAAATTC |
| 17jz | AcF_CUA_rev | 50 | 67.0 | CCCTGCCATGCGGATTCTAAGTCCGCCGTTCTGCCCTGCTGAACTACCGC |
| 17ka | AB_BB_gaF | 33 | 69.0 | TACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAA |
| 17kb | AB_BB_gaR | 48 | 69.0 | TACTTTCTCCTCTTTGTCGCTAGCATTGTACCTAGGACTGAGCTAGCC |
| 17kc | tRNA_heid_fw | 53 | 61.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGTGTGCTTCTCAAATGCCTGAGGC |
| 17kd | tRNA_heid_rev | 55 | 61.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTACATGCAAAAAAGCCTGCTCGTTGAG |
| 17ke | BB1C3_tRNA_h_fw | 51 | 62.0 | GCCTGCTCAACGAGCAGGCTTTTTTGCATGTACTAGTAGCGGCCGCTGCAG |
| 17kf | BB1C3_tRNA_h_re | 52 | 62.0 | CAAACTGGCCTCAGGCATTTGAGAAGCACACTCTAGAAGCGGCCGCGAATTC |
| 17kg | pSC101-pK18msb_seqF | 20 | 58.0 | GTTCCACTGAGCGTCAGACC |
| 17kh | pSC101-pK18msb_seqR | 20 | 56.0 | AACGCAGGAAAGAACATGTG |
| 17ki | TyrRS_pSB1C3_fw | 61 | 63.0 | GATTTTAGAGCCAATTAGAAAGAGATTATAACTGCAGTTTCAAACGCTAAATTGCCTGATG |
| 17kj | TyrRS_pSB1C3_rev | 63 | 63.0 | GTTATACGTTGTTTACGCTTTGAGGAATCCCATATGGACGAATTTGAAATGATAAAGAGAAAC |
| 17kk | PtNTT2_gaF_ext | 62 | 60.0 | CACTTTATGCTTCCGGCTCGTATAATGGACAAAGAGGAGAAAGTAATGCGCCCGTATCCGAC |
| 17kl | PtNTT2_gaR_ext | 68 | 56.0 | CTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTTTCTTTGTTGGTTTTCGCTTCC |
| 17km | LFS_Col | 27 | 58.0 | AATTTTCAGATGTGTTTTATCCGGGAG |
| 17kn | RFS_Col | 30 | 58.0 | GTGATAAATGGTAATGTTGCTCATATCAGT |
| 17ko | PtNTT2_trunc_gaF_v2 | 46 | 60.0 | TCGTATAATGGACAAAGAGGAGAAAGTAATGGGCGGCAGCACCGTG |
| 17kp | pSC101ori_ext_gaF | 43 | 64.0 | CCGTAGAAAAGATCAAAGGATCTTCAACGCCCTGAGAAGCCCG |
| 17kq | pSC101ori_ext_gaR | 47 | 63.0 | CGTAAAAAGGCCGCGTTGCTGGCGTGCATCGCCTGTTTCAGGCTGTC |
| 17kr | K80+RBS+T7RNAPre | 52 | 53.0 | CGTCTACATTACTAGTTTCTCCTCTTTGTTAGCCCAAAAAACGGGTATGGAG |
| 17ks | K80T7RNAPp1 | 61 | 53.0 | CCATACCCGTTTTTTGGGCTAACAAAGAGGAGAAACTAGTAATGTAGACGATTAACATCGC |
| 17kt | K808000+suff | 51 | 63.0 | CACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAG |
| 17ku | K808000+suf_re | 59 | 63.0 | CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCACCCG |
| 17kv | K808000+Term | 54 | 58.0 | ATCTTAGAGTCGGACTTCGCGTTCGCGTAACCAGGCATCAAATAAAACGAAAGG |
| 17kw | K808000+Term_re | 50 | 58.0 | GACTGAGCCTTTCGTTTTATTTGATGCCTGGTTACGCGAACGCGAAGTCC |
| 17kx | Pre+K808000 | 54 | 56.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGTTATGACAACTTGACGGCTACATC |
| 17ky | Pre+K808000_re | 50 | 56.0 | TAGCCGTCAAGTTGTCATAACTCTAGAAGCGGCCGCGAATTCCAGAAATC |
| 17kz | T7RNAP_pos107_re | 20 | 58.0 | TTGCAGGAACTGGAAGGCTG |
| 17la | T7RNAP_pos107_BL | 22 | 58.0 | TTCCTGCAATAGATCAAGCCGG |
| 17lc | I746909+suff_rev | 67 | 60.0 | TTGCCGGACTGCAGCGGCCGCTACTAGTATAATACGACTCACTATAGGGAGATACTAGAGAAAGAGG |
| 17ld | Pre+I746909re | 52 | 58.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGGCCCACC |
| 17le | pre+I746909re_rev | 55 | 58.0 | GTGGGCCTTTCTGCGTTTATACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
| 17lf | Seq_RFS | 20 | 66.0 | CGCCTGTGCCGCCCTTCAGA |
| 17lg | pTarget_seq_fwd | 20 | 54.0 | GCCTTTGAGTGAGCTGATAC |
| 17lh | pTarget_seq_rev | 20 | 55.0 | CGGCACTGTTGCAAATAGTC |
| 17li | I746909+suff | 49 | 60.0 | TCTAGTATCTCCCTATAGTGAGTCGTATTATACTAGTAGCGGCCGCTGC |
| 17lj | PtNTT2_seq_fwd | 23 | 58.0 | CCAGGAAGTGACCAAATTTCTGC |
| 17lk | PtNTT2_seq_rev | 19 | 60.0 | GCAGGCTCTGAATGGTGCC |
| 17ll | pSC101-pK18msb-seq3 | 20 | 59.0 | GAGGAGCAAACAGCGTTTGC |
| 17lm | pSC101-pK18msb-seq4 | 25 | 59.0 | ATCTGTGCATATGGACAGTTTTCCC |
| 17ln | sup35_pSB1C3_fw | 69 | 65.0 | GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGTCTGACTCTAACCAGGGTAACAACCAGCAGAAC |
| 17lo | sup35_pSB1C3_rv | 53 | 65.0 | GCCGCTACTAGTATTAAACTTCGTCGTCAACTTCTTCTTCCTGTTCTTTGATC |
| 17lp | TyrFS_pSB1C3_fw | 53 | 61.0 | CTTTGAGGAATCCCATATGGACGAATTTGAAATGATAAAGAGAAACACATCTG |
| 17lq | TyrFS_pSB1C3_rv | 67 | 61.0 | GCGTTTGAAACTGCAGTTATAATCTCTTTCTAATTGGCTCTAAAATCTTTATAAGTTCTTCAGCTAC |
| 17lr | sgRNA_BB_A_1_gF | 53 | 67.0 | TTCTATAGTCCTTGAGAGGAGGAGTTACTAGTAGCGGCCGCTGCAGGAGTCAC |
| 17ls | sgRNA_BB_G_2_gF | 58 | 67.0 | ATCAATACGTATACCAGGTGTCCTGTGAGCTACTAGTAGCGGCCGCTGCAGGAGTCAC |
| 17lt | sgRNA_BB_d_3_gF | 58 | 67.0 | CACGTATGAGAAGGTATTTGCCCGATAATCTACTAGTAGCGGCCGCTGCAGGAGTCAC |
| 17lu | sgRNA_BB_T_4_gF | 58 | 67.0 | AACTCCGGCAGGCAATTAAAGGGAACGTATTACTAGTAGCGGCCGCTGCAGGAGTCAC |
| 17lv | RomT_mutA_sense | 80 | nan | TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGAATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT |
| 17lw | RomT_mutA_anti | 80 | nan | AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATTCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA |
| 17lx | RomT_mutG_sense | 80 | nan | TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGGATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT |
| 17ly | RomT_mutG_anti | 80 | nan | AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATCCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA |
| 17lz | RomT_mutD_sense | 80 | nan | TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCTT |
| 17ma | RomT_mutD_anti | 80 | nan | AAGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA |
| 17mb | RomT_mutT_sense | 80 | nan | TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGTATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT |
| 17mc | RomT_mutT_anti | 80 | nan | AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATACTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA |
| 17md | RomT_mutC_sense | 80 | nan | TAGCTCAGTCCTAGGTACAGTGCTAGCCACACAATGTAAGCATCACGGTACTAGTGAAAGAGGAGAAATACTAGATGGCT |
| 17me | RomT_mutC_anti | 80 | nan | AGCCATCTAGTATTTCTCCTCTTTCACTAGTACCGTGATGCTTACATTGTGTGGCTAGCACTGTACCTAGGACTGAGCTA |
| 17mf | ownT_mutA_sense | 80 | nan | CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGACTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC |
| 17mg | ownT_mutA_anti | 80 | nan | GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGTCCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG |
| 17mh | ownT_mutG_sense | 80 | nan | CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGGCTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC |
| 17mi | ownT_mutG_anti | 80 | nan | GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGCCCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG |
| 17mj | ownT_mutT_sense | 80 | nan | CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGTCTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC |
| 17mk | ownT_mutT_anti | 80 | nan | GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGACCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG |
| 17ml | ownT_mutC_sense | 80 | nan | CTAGCTCAGTCCTAGGTACAGTGCTAGCGATGATAGGCGGCCTCTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC |
| 17mm | ownT_mutC_anti | 80 | nan | GCCATCTAGTATTTCTCCTCTTTCACTAGTACCGAAGAGGCCGCCTATCATCGCTAGCACTGTACCTAGGACTGAGCTAG |
| 17mn | pSC101-replace-fwd | 45 | 71.0 | TATGAAATTGGTGGTTGATAAGCGAGGCCGCCCGACTGATACGTT |
| 17mo | pSC101-replace-rev | 55 | 64.0 | GCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGG |
| 17mp | pK18msB_split_1 | 29 | 66.0 | GCAACTACAGCTCAGGCGACAACCATACG |
| 17mq | pK18msB_split_2 | 29 | 66.0 | CGTATGGTTGTCGCCTGAGCTGTAGTTGC |
| 17mr | sup_21TGT_fw | 50 | 62.0 | CCAGCAGTACTCTCAGAATGGATGTCAACAGCAAGGCAATAATCGTTATC |
| 17ms | sup_21TGT_rev | 70 | 63.0 | GTTGACATCCATTCTGAGAGTACTGCTGGTAATTCTGCTGGTTGTTACCCTGGTTAGAGTCAGACATCTC |
| 17mt | TyrRS_FluoAS_fw | 67 | 63.0 | GATTAAAATCCTGGAGCCCATTCGCAAGCGCCTGTAACTGCAGTTTCAAACGCTAAATTGCCTGATG |
| 17mu | TyrRS_FluoAS_rv | 70 | 63.0 | GATGTGTTACGCTTAATCATCTCGAACTCGTCCATATGGGATTCCTCAAAGCGTAAACAACGTATAACGG |
| 17mv | ONBY-out-rev_2 | 35 | 63.0 | ATGAATCTCCAAGTATATAACGACTGCACCTTTGC |
| 17mw | CLRS-fw | 40 | 73.0 | GAATTCGCGGCCGCTTCTAGAGTTTTAAAAAACTAACAGT |
| 17mx | CLRS-rv | 40 | 80.0 | CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCC |
| 17my | PrK_210CTT_fw | 41 | 69.0 | CCGAAAGACGAAATCAGCCTTAATTCCGGCAAACCGTTTCG |
| 17mz | PrK_210CTT_rv | 39 | 69.0 | AAGGCTGATTTCGTCTTTCGGATTCAGCAGAACCTCCAG |
| 17na | PrK_456CTT_fw | 46 | 69.0 | CTTTAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGG |
| 17nb | PrK_456CTT_rv | 54 | 69.0 | CAATTTAGCGTTTGAAACTAAAGTTACAGGTTCGTAGAGATCCCGTTATAATAC |
| 17nc | AcF_308CTT_fw | 46 | 69.0 | CTTCAGTTTCAAACGCTAAATTGCCTGATGCGCTACGCTTATCAGG |
| 17nd | AcF_308CTT_rv | 54 | 69.0 | CAATTTAGCGTTTGAAACTGAAGTTATAATCTCTTTCTAATTGGCTCTAAAATC |
| 17ne | AcF_107GAG_fw | 59 | 69.0 | AGTGAGTTCCAGCTTGATAAGGATTATACACTGAATGTCTATAGATTGGCTTTAAAAAC |
| 17nf | AcF_107GAG_rv | 56 | 69.0 | GTGTATAATCCTTATCAAGCTGGAACTCACTTCCATAAACATATTTTGCCTTTAAC |
| 17ng | sup35_121CTA_fw | 51 | 70.0 | CAAGCTGGATTCCAGCCTCAACTACAGGGGATGTCGCTTAATGACTTTCAG |
| 17nh | sup35_121CTA_rv | 49 | 70.0 | CCCCTGTAGTTGAGGCTGGAATCCAGCTTGATAACCTTGCAAATTGTTG |
| 17ni | sup35_Histag_rev | 73 | 70.0 | TTAATGGTGATGATGGTGGTGATGGTGATGATGAACTTCGTCGTCAACTTCTTCTTCCTGTTCTTTAATTAAC |
| 17nj | blocking_L_fw | 42 | 72.0 | CGGCCTGCTGACTTTCTCGCCGATCAAAAGGCATTTTGCTAT |
| 17nk | blocking_L_rv | 37 | 72.0 | GCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCC |
| 17nl | blocking_R_fw | 72 | 70.0 | GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCG |
| 17nm | blocking_R_rv | 48 | 70.0 | AAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTAAAAAAAGCACCG |
| 17nn | sgRNA_biobrck_F | 44 | 63.0 | CTAGCATCTAGTGACTCTAGAGCTAAGCGGCCTGCTGACTTTCT |
| 17no | sgRNA_biobrck_R | 59 | 63.0 | GAACTAGACAGATTCTGCAGCGGCCGCTACTAGTAAAAAAAGCCTGCTCGTTGAGCAGG |
| 17np | FluoAS_308CTT | 67 | 69.0 | CATCAGGCAATTTAGCGTTTGAAACTGAAGTTACAGGCGCTTGCGAATGGGCTCCAGGATTTTAATC |
| 17nq | Prüf_ori_fw_58 | 32 | 58.0 | CCTTTTTCTTTAAAACCGAAAAGATTACTTCG |
| 17nr | Prüf_ori_rev_58 | 22 | 58.0 | CGGGGCGTAATTTGATATCGAG |
| 17ns | NPA_seq_fwd | 19 | 56.0 | CCATTTGGGTGATCTTGGC |
| 17nt | NPA_seq_rev | 24 | 55.0 | CAAGGATTCTAACTCCTCATACGA |
| 17nu | GFP_tag_gaF | 55 | 58.0 | GAACAAAAGCTTATTTCTGAAGAGGACTTGCGTAAAGGAGAAGAACTTTTCACTG |
| 17nv | GFP_tag_gaR | 57 | 54.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATTATTTGTATAGTTCATCCATGCCATG |
| 17nw | PtNTT2_tag_gaR | 55 | 56.0 | CAAGTCCTCTTCAGAAATAAGCTTTTGTTCTTTTTCTTTGTTGGTTTTCGCTTCC |
| 17nx | KanR_fw | 54 | 55.0 | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGAGCCATATTCAACGGG |
| 17ny | KanR_rev | 49 | 53.0 | GCCGGACTGCAGCGGCCGCTACTAGTATTAGAAAAACTCATCGAGCATC |
| 17nz | KanR_S133Am | 23 | 63.0 | GCGCCGGTTGCATTAGATTCCTG |
| 17oa | KanR_S133Am_rev | 19 | 63.0 | GCGCAGGAACACTGCCAGC |
| 17ob | KanR_S154Am | 26 | 63.0 | CAGGCGCAATAGCGAATGAATAACGG |
| 17oc | KanR_S154Am_rev | 23 | 65.0 | GCCTGAGCGAGACGAAATACGCG |
| 17od | pSB1C3_PF_rev | 52 | 60.0 | CTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATCTCTAGAAGCGGCCGCG |
| 17oe | pSB1C3_SF_fw | 54 | 60.0 | TGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATACTAGTAGCGGCCGCTGC |
| 17of | T7RNAP_BL_fw | 20 | 62.0 | CAGACATTGCCGTCACTGCG |
| 17og | T7RNAP_BL_rev | 17 | 61.0 | GCCACGAAGACCACGCC |
| 17oh | T7_CBS_fw | 63 | 63.0 | GACTCACTATAGGGAATACAAGCTACTTGTTCTTTTTGCATACTAGTAGCGGCCGCTGCAGTC |
| 17oi | T7_CBS_rev | 72 | 63.0 | CAAGTAGCTTGTATTCCCTATAGTGAGTCGTATTACTCTAGTATTAGCTATTCAGATTTGCGATACACCACG |
| 17oj | KanR_rev_fw | 56 | 52.0 | GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTAGAAAAACTCATCGAGCATC |
| 17ok | KanR_rev_rev | 54 | 52.0 | CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTAATGAGCCATATTCAACGG |
| 17ol | mutT_flanke1_gaF | 50 | 59.0 | TAATGTGAGTTAGCTCACTCATTAGGCACCCTCGATTTGCCAATTGCCGA |
| 17om | mutT_flanke1_gaR | 55 | 55.0 | GTCGGATGCGGCGAAAACGCCTTATCTGACTGTCTTAAACCTATAAAAAAGGCGC |
| 17on | mutT-flanke2_gaF | 50 | 56.0 | ATTCTGCGCCTTTTTTATAGGTTTAAGACAGTCAGATAAGGCGTTTTCGC |
| 17oo | mutT-flanke2-gaR | 49 | 54.0 | CTGCGGACTGGCTTTCTACGTGTTCCGCTTGGCACTTACCATGGTGCTG |
| 17op | Cas9only_F | 64 | 58.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGATAAGAAATACTCAATAGGCTTAGATATCG |
| 17oq | Cas9only_R | 51 | 58.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATCAGTCACCTCCTAGCTGACT |
| 17or | bb_Cas9only_F | 55 | 67.0 | ATTGATTTGAGTCAGCTAGGAGGTGACTGATACTAGTAGCGGCCGCTGCAGTCCG |
| 17os | bb_Cas9only_R | 60 | 66.0 | ATCTAAGCCTATTGAGTATTTCTTATCCATCTCTAGAAGCGGCCGCGAATTCCAGAAATC |
| 17ot | crtI_86TAG_fw | 51 | 68.0 | GAACTGCTGCCGGTTACGCCGTAGTACCGCCTGTGTTGGGAGTCAGGGAAG |
| 17ou | crtI_86TAG_rev | 64 | 68.0 | ACGGCGTAACCGGCAGCAGTTCGACATACTCTTTTAACTGTTTTCCTGCCAGTGCAAACAGTTC |
| 17ov | crtI_318TAG_fw | 55 | 68.0 | CTATTTTGGTTTGAATCACCATCATTAGCAGCTCGCGCATCACACGGTTTGTTTC |
| 17ow | crtI_318TAG_rev | 61 | 68.0 | ATGATGGTGATTCAAACCAAAATAGAGCACAAACAGAGAGTTACTCATGCGCTTAGTTTGC |
| 17ox | crtI_353TAG_fw | 49 | 68.0 | GAGGACTTCTCACTTTATCTGTAGGCGCCCTGTGTCACGGATTCGTCAC |
| 17oy | crtI_353TAG_rev | 60 | 68.0 | CAGATAAAGTGAGAAGTCCTCTGCGAGGCCATCATGATTAAAAATTTCGTCAATCAGCTC |
| 17oz | BB_ovexGA_F | 18 | 52.0 | GATTTCTGGAATTCGCGG |
| 17pa | BB_ovexGA_R | 15 | 52.0 | TTTGCCGGACTGCAG |
| 17pb | B0015_ovex_F | 54 | 57.0 | ATTGATTTGAGTCAGCTAGGAGGTGACTGACCAGGCATCAAATAAAACGAAAGG |
| 17pc | B0015_ovex_R | 51 | 57.0 | AATGGTAATGTTGCTCATATCAGTATCTCATATAAACGCAGAAAGGCCCAC |
| 17pd | B0015_GA_F | 54 | 57.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGCCAGGCATCAAATAAAACGAAAGG |
| 17pe | B0015_GA_R | 51 | 57.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCCCAC |
| 17pf | Cas9o_ovex_F | 68 | 57.0 | TGTGAGCGGATAACAATTTCACACAGGAATACTAGATGGATAAGAAATACTCAATAGGCTTAGATATC |
| 17pg | Cas9o_ovex_R | 50 | 56.0 | ACTGAGCCTTTCGTTTTATTTGATGCCTGGGTCAGCTAGGAGGTGACTGA |
| 17ph | RFS_ovex_F | 57 | 56.0 | CACCTTCGGGTGGGCCTTTCTGCGTTTATATGAGATACTGATATGAGCAACATTACC |
| 17pi | RFS_GA57_R | 60 | 57.0 | TTTGCCGGACTGCAGCGGCCGCTACTAGTACAATAAATGTAATGCGTTATATCTTCCTGA |
| 17pj | Seq_crtE1_fw | 22 | 58.0 | CTACGAGGATCAGGGGTTTACC |
| 17pk | Seq_crtE2_fw | 22 | 58.0 | GTGGTTCATACCTATCGCGACC |
| 17pl | Cas9o_ovex_R2 | 50 | 56.0 | ACTGAGCCTTTCGTTTTATTTGATGCCTGGTCAGTCACCTCCTAGCTGAC |
| 17pm | 28567c3g1_gF | 64 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCCTTGGATCCTAAAGTGAAG |
| 17pn | 28567c3g1_gR | 61 | 59.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCATTCCCATTCGATGGTTGATGG |
| 17po | 27205c6g2i2_gF | 63 | 58.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCTTTAGAATTGCAAGACGG |
| 17pp | 27205c6g2i2_gR | 67 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAGAAGGCCTTTTTCTCATAAGAAATGAG |
| 17pq | 24513c0g1_gF | 65 | 58.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCTTCTTCAGCTTCCAATAATG |
| 17pr | 24513c0g1i3_gR | 73 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAGAGAAAAGTCGTTTTTCATATGAGTATAAACC |
| 17ps | 24513c0g1i1_gR | 73 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAGAATAGAGACGTTTTTCATATGAGTATAAACC |
| 17pt | 27205c6g2i4_gR | 63 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCATGTAGGGAACATCCAGAAGCTTA |
| 17pu | 107595c0g1i1_gF | 64 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGAACCTCAATTCTCTCCGCCTC |
| 17pv | 107595c0g1i1_gR | 64 | 59.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCACTTGTATATCAAGGCATCTCGTCC |
| 17pw | 30924c1g1i14_gF | 63 | 58.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGGGTCGTTGAAAAATGAAGG |
| 17px | 30924c1g1i14_gR | 64 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAACACTAAGCTTGGGATGGTAATC |
| 17py | 30983c0g1i12_gF | 65 | 61.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGTGTTCCAAAAAATAGCAGCCC |
| 17pz | 30983c0g1i12_gR | 57 | 61.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCACTTGCCGCCCGTTTTCC |
| 17qa | 26152c4g1i4_gF | 75 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAAAACGAAAACAAAATAGAAAGTAACAGTAG |
| 17qb | 26152c4g1i4_gR | 68 | 59.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCATTTACCATCCATTTTCCACTGTAAGTAC |
| 17qc | 29270c2g2i7_gF | 64 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAAGGTCAATATCCACAGCAG |
| 17qd | 29270c2g2i7_gR | 67 | 59.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCACACAAGCTGCACAACAGCAGCCACTCG |
| 17qe | 30924c1g1i5_gR | 60 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAAAGCAGTTCCATCCGTGTAG |
| 17qf | 26152c4g1i8_gF | 63 | 58.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAGAATCAAACTGCTCTTGC |
| 17qg | L_rob_pSB1C3_fw_65 | 52 | 65.0 | CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTGGTCCTGGCCACGGGTGC |
| 17qh | L_pSB1C3_rop_rev_65 | 49 | 65.0 | GCCGCGTTGCTGGCGTTGGTCCTGGCCACGGGTGCGCATGATCGTGCTC |
| 17qi | k_rop_pSB1C3_fw_66 | 53 | 65.0 | CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTGCGGAAGTCAGCGCCCTGC |
| 17qj | k_pSB1C3_rop_rev_65 | 50 | 65.0 | CGGAACATAATGGTGCAGGGCGCTGACTTCCGCAACGCCAGCAACGCGGC |
| 17qk | Cas9_EcoRI_F | 55 | 67.0 | AGACAATCGTGAGAAGATTGAAAAAATCTTGACTTTTCGCATCCCTTATTATGTT |
| 17ql | Cas9_EcoRI_R | 50 | 73.0 | TTGCCACGCGCCAATGGACCAACATAATAAGGGATGCGAAAAGTCAAGAT |
| 17qm | 28626c0g1i1_gF | 65 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAGCAGTACAGATTCGTAATCG |
| 17qn | 28626c0g1i1_gR | 65 | 58.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCAACAGGACTTATTAGAACTGCAATGG |
| 17qo | CLRS-fw_OL | 50 | 77.0 | TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTTTAAAA |
| 17qp | CLRS-rv_OL | 50 | 83.0 | CCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATATAAACGC |
| 17qq | gRNA_fw_codA | 25 | 0.0 | TAGC CACAGTGAACGTCGATGAGA |
| 17qr | gRNA_rv_codA | 25 | 0.0 | AAAC TCTCATCGACGTTCACTGTG |
| 17qs | pSB3K5-UBP-gaF | 19 | 60.0 | GCCGCTGCAGGAGTCACTA |
| 17qt | pSB3K5-UBP-gaR | 22 | 59.0 | CCGCGAATTCGAGTCACTAAGG |
| 17qu | mRFP-UBP-gaF | 22 | 59.0 | CCCTTAGTGACTCGAATTCGCG |
| 17qv | mRFP-Link-ccdB-gaR | 43 | 63.0 | AAACCTTAAACTGTGCGGCTGCCGCAGCACCGGTGGAGTGACG |
| 17qw | ccdB-Linker-mRFP-gaF | 58 | 60.0 | CTCCACCGGTGCTGCGGCAGCCGCACAGTTTAAGGTTTACACCTATAAAAGAGAGAGC |
| 17qx | ccdB-UBP_gaR | 60 | 60.0 | TAGTGACTCCTGCAGCGGCCGCTACTAGTATTATATTCCCCAGAACATCAGGTTAATGGC |
| 17qy | ccdB-UBP_gaF | 63 | 59.0 | TAGTGAAAGAGGAGAAATACTAGATGGCTTCAGTTTAAGGTTTACACCTATAAAAGAGAGAGC |
| 17qz | mRFP-UBP_gaR | 47 | 62.0 | GTGGCTAGCACTGTACCTAGGACTGAGCTAGCACCGGTGGAGTGACG |
| 17ra | PtNTT2_bb_gaR | 32 | 59.0 | CATTACTTTCTCCTCTTTGTCCATTATACGAG |
| 17rb | PtNTT2-noSP_gaF | 52 | 61.0 | CTCGTATAATGGACAAAGAGGAGAAAGTAATGCTGCCGGTGAAAAAAGGCAC |
| 17rc | CLtRNA_fw | 47 | 72.0 | TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTGTGC |
| 17rd | CLtRNA_rv | 30 | 72.0 | GCCGCTGCAGTCCGGCAAAAAAGGGCAAGG |
| 17re | RDL_SELP_fw | 50 | 72.0 | TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAGAGGAGG |
| 17rf | RDL_SELP_rv | 30 | 72.0 | CTTGCCCTTTTTTGCCGGACTGCAGCGGCC |
| 17rg | XbaI_RBS_baBL_fw | 51 | 60.0 | CTTCTAGAGAAAGAGGAGAAACTAGTAATGGCAGCATAGGTTATCAACACG |
| 17rh | SpeI_baBL_rev | 45 | 60.0 | GCTACTAGTATTATCTGATTTTTGTAAAGGTCTGATAATGGTCCG |
| 17ri | Rubisco_110ATT_fw | 46 | 57.0 | CGCGGCAATTAGTAAGATATCAAAGAGGAGAAATACTAGATGGCAG |
| 17rj | Rubisco_110ATT_rv | 41 | 57.0 | GATATCTTACTAATTGCCGCGGTAGACCACGAAGGCCAGAC |
| 17rk | BcuI_R | 40 | 68.0 | TCCTCTTTCACAAGAAGCTAGCACTGTACCTAGGACTGAG |
| 17rl | BcuI_F | 40 | 66.0 | TGCTAGCTTCTTGTGAAAGAGGAGAAATACTAGATGGCTT |
| 17rm | MHA_GA_F | 60 | 69.0 | ATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGC |
| 17rn | MHA_GA_R | 70 | 68.0 | CGTATAACGTTACTGGTTTCATCTAGTATTTCTCCTCTTTCACAAGAAGCTAGCACTGTACCTAGGACTG |
| 17ro | lacI_BB_R | 62 | 68.0 | TACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTA |
| 17rp | lacI_BB_F | 67 | 69.0 | TCTTGTGAAAGAGGAGAAATACTAGATGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGT |
| 17rq | fwd_CDD_pSB1C3 | 56 | 61.0 | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGATGCAAGCCAAACCATTTCAC |
| 17rr | rev_CDD_pSB1C3 | 71 | 62.0 | CCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATCAATTGAAATTTAAATCTAATGTATCAATTGAATC |
| 17rs | Split_RBS_F | 45 | 60.0 | TACTAGTGAAAGAGGAGAAATACTAGAGATGGCTTCCTCCGAAGA |
| 17rt | Split_RBS_R | 46 | 59.0 | TTTCTCCTCTTTCACTAGTATGTGTGAAATTGTTATCCGCTCACAA |
| 17ru | PtNTT2-pelB-SP_gaF | 65 | 59.0 | GCGGCGGCGGGCCTGCTGCTGCTGGCGGCGCAGCCGGCGATGGCGCTGCCGGTGAAAAAAGGCAC |
| 17rv | PtNTT2_bb_pelB-SP_gaR | 74 | 55.0 | CAGCAGCAGCAGGCCCGCCGCCGCGGTCGGCAGCAGATATTTCATTACTTTCTCCTCTTTGTCCATTATACGAG |
| 17rw | PtNTT2_TAT-SP_gaF | 77 | 56.0 | ACCACAGGACTTGCCACTATCGGCGCTGCCAGCATGTTTATGCCAAAGGCCAACGCCCTGCCGGTGAAAAAAGGCAC |
| 17rx | PtNTT2_bb_TAT-SP_gaR | 86 | 60.0 | GCCGATAGTGGCAAGTCCTGTGGTGGCTTTTAAGAAGCCTCGGCGGTTTATTTGCATTACTTTCTCCTCTTTGTCCATTATACGAG |
| 17ry | PtNTT2-only_gaF | 43 | 63.0 | CTGGAATTCGCGGCCGCTTCTAGAGATGCGCCCGTATCCGACC |
| 17rz | fwd_CDD_SpeI | 50 | 50.0 | TGATGAAAAATGTGGCTAATACCAGTGTTTTGAAATGGGGTGGAATATTG |
| 17sa | rev_CDD_SpeI | 76 | 76.0 | AATATTCCACCCCATTTCAAAACACTGGTATTAGCCACATTTTTCATCACCTTGGTATTTCCAAATTTCTTACCTC |
| 17sb | AcF_107GAG2_fw | 62 | 64.0 | GATAAGGATTATACACTGAATGTCTATAGATTGGCTTTAAAAACTACCTTAAAAAGAGCAAG |
| 17sc | AcF_107GAG2_rv | 48 | 64.0 | GACATTCAGTGTATAATCCTTATCAAGCTGGAACTCACTTCCATAAAC |
| 17sd | PtNTT2-lacO-gaR | 53 | 55.0 | TCGACTGAGCCTTTCGTTTTATTTGTTATTTTTCTTTGTTGGTTTTCGCTTCC |
| 17se | lacO-PtNTT2_gaF | 50 | 59.0 | AAGCGAAAACCAACAAAGAAAAATAACAAATAAAACGAAAGGCTCAGTCG |
| 17sf | lacO-PtNTT2_gaR | 54 | 57.0 | ATCTAAGCCTATTGAGTATTTCTTATCCATTTCCTGTGTGAAATTGTTATCCGC |
| 17sg | Cas9_gaF | 63 | 56.0 | TTGTGAGCGGATAACAATTTCACACAGGAAATGGATAAGAAATACTCAATAGGCTTAGATATC |
| 17sh | is_kan_fw | 44 | 61.0 | CCAATTAACCAATTCTGAATGATTGAACAAGATGGATTGCACGC |
| 17si | is_kan_rev | 53 | 61.0 | CATAAACAGTAATACAAGGGGTGTTTCAGAAGAACTCGTCAAGAAGGCGATAG |
| 17sj | bb_kan_fv | 56 | 61.0 | CGCCTTCTTGACGAGTTCTTCTGAAACACCCCTTGTATTACTGTTTATGTAAGCAG |
| 17sk | bb_kan_rv | 53 | 61.0 | GCAATCCATCTTGTTCAATCATTCAGAATTGGTTAATTGGTTGTAACACTGGC |
| 17sl | pSB_CFP_fw | 54 | 69.0 | GTCACTCCACCGGTGCTTAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG |
| 17sm | pSB_YFP_rev | 60 | 69.0 | CAGTTCTTCGCCTTTGCTCATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGC |
| 17sn | CFP_YFP_fw | 50 | 63.0 | GAATTCGCGGCCGCTTCTAGAGATGAGCAAAGGCGAAGAACTGTTTACCG |
| 17so | CFP_pSB_rev | 64 | 63.0 | GAACCAGCAGCGGAGCCAGCGGATCCCATTTTATACAGTTCATCCATGCCATGGGTAATACCGG |
| 17sp | YFP_CFP_fw | 67 | 63.0 | GGGATCCGCTGGCTCCGCTGCTGGTTCTGGCGAATAGATGGCTTCCTCCGAAGACGTTATCAAAGAG |
| 17sq | YFP_pSB_rev | 53 | 63.0 | CCGGACTGCAGCGGCCGCTACTAGTATTAAGCACCGGTGGAGTGACGACCTTC |
| 17sr | fwd_CCD_Topo | 25 | 60.0 | CCGTTTCTCATCGAATATGCAAGCC |
| 17ss | rev_CCD_Topo | 26 | 61.0 | GCCCAAAAACCATGAAATCCAAGTGG |
| 17st | fwd_pTXB1_backb | 21 | 65.0 | TGCATCACGGGAGATGCACTA |
| 17su | rev_pTXB1_backb | 45 | 66.0 | ATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGG |
| 17sv | pC3_YFP_fw | 49 | 69.0 | GATGAACTGTATAAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAG |
| 17sw | pC3_CFP_rv | 51 | 69.0 | GGAGGAAGCCATCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGC |
| 17sx | YFP_pC3_fw | 60 | 63.0 | GATTTCTGGAATTCGCGGCCGCTTCTAGAGATGGCTTCCTCCGAAGACGTTATCAAAGAG |
| 17sy | YFP_CFP_rv | 52 | 63.0 | CAGAACCAGCAGCGGAGCCAGCGGATCCCATAGCACCGGTGGAGTGACGACC |
| 17sz | CFP_YFP_fw | 56 | 63.0 | TGGCTCCGCTGCTGGTTCTGGCGAATAGATGAGCAAAGGCGAAGAACTGTTTACCG |
| 17ta | CFP_pC3_rev | 58 | 63.0 | GGACTGCAGCGGCCGCTACTAGTATTTATACAGTTCATCCATGCCATGGGTAATACCG |
| 17tb | B0015_ba_fw | 58 | 63.0 | CCATTATCAGACCTTTACAAAAATCAGATAACCAGGCATCAAATAAAACGAAAGGCTC |
| 17tc | B0015_ba_r_rev | 64 | 63.0 | CGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGGCCCACCCG |
| 17td | baBL_fw | 67 | 60.0 | CTCCATACCCGTTTTTTGGGCTAACAAAGAGGAGAAACTACTAATGGCAGCATAGGTTATCAACACG |
| 17te | baBL_rev | 70 | 60.0 | CGACTGAGCCTTTCGTTTTATTTGATGCCTGGTTATCTGATTTTTGTAAAGGTCTGATAATGGTCCGTTG |
| 17tf | K80_ba_rev | 70 | 56.0 | GATAACCTATGCTGCCATTAGTAGTTTCTCCTCTTTGTTAGCCCAAAAAACGGGTATGGAGAAACAGTAG |
| 17tg | K808000_ba_r_rev | 60 | 56.0 | CCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGTATTATGACAACTTGACGGCTACATC |
| 17th | pSB1C3_ba_r_fw | 66 | 63.0 | GGTTGTGAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAATACTAGTAGCGGCCGCTGCAG |
| 17ti | pSB1C3_ba_r_rev | 64 | 63.0 | GGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATACTCTAGAAGCGGCCGCGAATTCCAGAAATC |
| 17tj | pSB1C3_prom_fw | 69 | 65.0 | CCTCTTTGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAATACTAGTAGCGGCCGCTGCAGTCCGGC |
| 17tk | Term_kanR_rev | 69 | 54.0 | CCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGGCCC |
| 17tl | Term_kanR_fw | 53 | 54.0 | GCAGTTTCATTTGATGCTCGATGAGTTTTTCTAACCAGGCATCAAATAAAACG |
| 17tm | pSB1C3_term_rev | 70 | 65.0 | CTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |
| 17tn | kanR_term_rev | 71 | 55.0 | GGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGTTAGAAAAACTCATCGAGCATCAAATG |
| 17to | kanR_prom_fw | 70 | 55.0 | GACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCAAAGAGGAGAAAGTAGTAATGAGCCATATTCAACGGG |
| 17tp | SpeI_weg_fw | 24 | 54.0 | GAGGAGAAAGTAGTAATGTAGACG |
| 17tq | SpeI_weg_rev | 21 | 54.0 | CTTTCTCCTCTTTGTTAGCCC |
| 17tr | qPCR_mRFP_F | 23 | 62.0 | AAGGTCGTCCGTACGAAGGTACC |
| 17ts | qPCR_mRFP_R | 25 | 63.0 | TCAGCCGGGTGTTTAACGTAAGCTT |
| 17tt | Cm_pSB6A1_fw_53 | 42 | 53.0 | GAGTAAACTTGGTCTGACAGGATTCTCACCAATAAAAAACGC |
| 17tu | Cm_pSB6A1_rev_53 | 46 | 53.0 | GTTAATGTCATGATAATAATGGTTTCTTAGACCGTCAGGTGGCACT |
| 17tv | pSB6A1_Cm_fw_52 | 53 | 52.0 | CCCGATCAACTCGAGTGCCACCTGACGGTCTAAGAAACCATTATTATCATGAC |
| 17tw | pSB6A1_Cm_rev_52 | 54 | 52.0 | GGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCTGTCAGACCAAGTTTACTC |
| 17tx | mRFP-UBP-New_gaR | 47 | 62.0 | ATCGCTAGCACTGTACCTAGGACTGAGCTAGCACCGGTGGAGTGACG |
| 17ty | ccdB-UBP-Max_gaF | 64 | 58.0 | ACTAGTGAAAGAGGAGAAATACTAGATGGCTCAGTTTAAGGTTTACACCTATAAAAGAGAGAGC |
| 17tz | ccdB-UBP-New_gaF | 64 | 59.0 | ATAGTGAAAGAGCAGAAATACTAGATGGCTTCAGTTTAAGGTTTACACCTATAAAAGAGAGAGC |
| 17ua | codA_lin_fwd | 21 | 59.0 | CATTAAAAGCGGTCGTTCCCG |
| 17ub | codA-lin_rev | 20 | 59.0 | CGGAATTTGCCACCAGAGAG |
| 17uc | mRFP1_CBT-Asp_fw | 35 | 70.0 | TTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCT |
| 17ud | mRFP1_CBT-Asp_rv | 26 | 70.0 | CCTTTTTTGCCGGACTGCAGCGGCCG |
| 17ue | Cas9_Split_F | 32 | 61.0 | ATCTTGACTTTTCGCATCCCTTATTATGTTGG |
| 17uf | Cas9_Split_R | 32 | 61.0 | CCAACATAATAAGGGATGCGAAAAGTCAAGAT |
| 17ug | RBS_tight_F | 43 | nan | TTTCACACAGGAATACTAGATGGCTTCCTCCGAAGACGTTATC |
| 17uh | RBS_tight_R | 41 | nan | CATCTAGTATTCCTGTGTGAAATTGTTATCCGCTCACAATT |
| 17ui | lacUV5_split_F | 35 | nan | TATGCTTCCGGCTCGTATAATGTGTGAAATTGTGA |
| 17uj | lacUV5_split_R | 35 | nan | TCACAATTTCACACATTATACGAGCCGGAAGCATA |
| 17uk | RBS_B0034_F | 49 | nan | ACAATTAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCA |
| 17ul | RBS_B0034_R | 47 | nan | CATCTAGTATTTCTCCTCTTTAATTGTTATCCGCTCACAATTTCACA |
| 17um | RBS_B0035_F | 52 | nan | AATTATTAAAGAGGAGAATACTAGATGGCTTCCTCCGAAGACGTTATCAAAG |
| 17un | RBS_B0035_R | 49 | nan | CATCTAGTATTCTCCTCTTTAATAATTGTTATCCGCTCACAATTTCACA |
| 17uo | kan_alt_bb_1 | 23 | 64.0 | TACTAGTAGCGGCCGCTGCAGTC |
| 17up | kan_alt_bb_2 | 18 | 63.0 | CCCTTCGGGAAGCGTGGC |
| 17uq | kan_alt_bb_3 | 21 | 63.0 | GGTCTGACAGCTCGAGTCCCG |
| 17ur | kan_alt_bb_4 | 24 | 61.0 | CCGTTATTCATTCGTGATTGCGCC |
| 17us | kan_alt_bb_5 | 32 | 60.0 | CACCCCTTGTATTACTGTTTATGTAAGCAGAC |
| 17ut | pos_kanRr_fw | 53 | 60.0 | AGCCTGCTCAACGAGCAGGCTTTTTTGCATGTATAAACGCAGAAAGGCCCACC |
| 17uu | pos_B0015_rev | 63 | 60.0 | CACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATATAAACGCAGAAAGGCCCACCC |
| 17uv | pos_kanRr_rev | 55 | 60.0 | AAGTGAATGATGTAGCCGTCAAGTTGTCATAATTGACGGCTAGCTCAGTCCTAGG |
| 17uw | pos_T7RNAP_fw | 69 | 60.0 | CTTTGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAATTATGACAACTTGACGGCTACATCATTCAC |
| 17ux | pos_tRNA_rev | 55 | 60.0 | CACCTTCGGGTGGGCCTTTCTGCGTTTATACATGCAAAAAAGCCTGCTCGTTGAG |
| 17uy | pSC_mRFP_fw | 55 | 68.0 | GTGCTAGTGTAGATCGCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAG |
| 17uz | pSC_mRFP_rev | 67 | 68.0 | CTCCTCTTTCTCTAGTATCTCCCTTTAGTGAGTGTTAATTTACTAGTAGCGGCCGCTGCAGTCCGGC |
| 17va | mRFP_pSC_fw | 64 | 63.0 | GGGAGATACTAGAGAAAGAGGAGAAATACTAGAGATGGCTTCCTCCGAAGACGTTATCAAAGAG |
| 17vb | mRFP_pSC_rev | 49 | 63.0 | CGGCCGCTTCTAGAGGCGATCTACACTAGCACTATCAGCGTTATTAAGC |
| 17vc | T3Pol_2TAG_fw | 55 | 63.0 | ATGTAGTAGATCATCGAAAACATCGAAAAGAATGACTTCTCAGAAATCGAACTGG |
| 17vd | T3Pol_2TAG_rev | 67 | 63.0 | CGATGTTTTCGATGATCTACTACATCTAGTACTTTCCTGTGTGACTCTAGTATTAGAAAAACTCATC |
| 17ve | Bb-pRS_RFP_fw_61 | 24 | 61.0 | GTTGATGTTGCTGTTGGAGGGATG |
| 17vf | Bb-pRS_RFP_rev_61 | 37 | 61.0 | CAACCTTTGGATTTTCATCCTCTCTTGCTATAAGTTC |
| 17vg | mRFP_RS_fw_60 | 58 | 60.0 | GAACTTATAGCAAGAGAGGATGAAAATCCAAAGGTTGCAATACGCAAACCGCCTCTCC |
| 17vh | mRFP_RS_rev_60 | 50 | 60.0 | CTCCATCCCTCCAACAGCAACATCAACTATAAACGCAGAAAGGCCCACCC |
| 17vi | 158_159_162 _fw 55 | 73 | 55.0 | GAGTATGGAACTTATAGCAAGAGAGGATGAAAATCCAAAGGTTGCTGAAGTTATCTATCCAATAATGATGGTT |
| 17vj | 158-159-162 rev 55 | 77 | 55.0 | CTCCATCCCTCCAACAGCAACATCAACGCCMNNATAATGCAGMNNMNNAACCATCATTATTGGATAGATAACTTCAG |
| 17vk | S133_S155_fw_59 | 74 | 59.0 | GCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCTAATGCAACCGGCGCAG |
| 17vl | S155_S133_rev_59 | 61 | 59.0 | GCGATCGCGTATTTCGTCTCGCTCAGGCGCAATAGCGAATGAATAACGGTTTGGTTGATGC |
| 17vm | Prüf_S155_S133_rev_59 | 18 | 59.0 | CTGCGCCGGTTGCATTAG |
| 17vn | T7RNAP_spe_fw_59 | 63 | 59.0 | CCGTTTTTTGGGCTAACAAAGAGGAGAAAGTAGTAATGTAGACGATTAACATCGCTAAGAACG |
| 17vo | T7RBS_Spe_rev_61 | 62 | 61.0 | CGTTCTTAGCGATGTTAATCGTCTACATTACTACTTTCTCCTCTTTGTTAGCCCAAAAAACG |
| 17vp | T7RNAP_107_fw_59 | 48 | 59.0 | GCCCGACAGCCTTCCAGTTCCTGCAATAGATCAAGCCGGAAGCCGTAG |
| 17vq | T7RNAP_107_rev_59 | 52 | 59.0 | GTGATGTACGCTACGGCTTCCGGCTTGATCTATTGCAGGAACTGGAAGGCTG |
| 17vr | Prüf_T7RNAP_107_rev_59 | 19 | 59.0 | CGGCTTCCGGCTTGATCTA |
| 17vs | gBlock_delta_own2_s | 18 | 56.0 | GTGAGCGATGCTCCGTTG |
| 17vt | UBP_PCR_1C3_fw | 21 | 56.0 | CCTTAGCTTTCGCTAAGGATG |
| 17vu | UBP_PCR_1C3_rv | 19 | 56.0 | CGAATTGCAAGACGCTGTC |
| 17vv | Prefix-AG_rev | 20 | nan | CTAGAAGCGGCCGCGAATTC |
| 17vw | PtNTT2-AG_gaF | 43 | nan | TTCTGGAATTCGCGGCCGCTTCTAGATGCGCCCGTATCCGACC |
| 17vx | codA-flank-part_gaF | 46 | nan | CTGGAATTCGCGGCCGCTTCTAGAGCATTAAAAGCGGTCGTTCCCG |
| 17vy | codA-flank-part_gaR | 45 | nan | CGGACTGCAGCGGCCGCTACTAGTACGGAATTTGCCACCAGAGAG |
| 17vz | mRFP_sacB_gaR | 55 | nan | CTTGTTTTGCAAACTTTTTGATGTTTGCGGCTGCCGCAGCACCGGTGGAGTGACG |
| 17wa | sacB_ccdB-rep_gaF | 57 | nan | CGTCACTCCACCGGTGCTGCGGCAGCCGCAAACATCAAAAAGTTTGCAAAACAAGCA |
| 17wb | sacB-ccdB-rep_gaR | 63 | nan | TAGTGACTCCTGCAGCGGCCGCTACTAGTATTATTTGTTAACTGTTAATTGTCCTTGTTCAAG |
| 17wc | alt_VR_pSB1C3 | 21 | 57.0 | CCTGCATAACGCGAAGTAATC |
| 17wd | sacB-UBP-Max_gaF | 60 | nan | GTACTAGTGAAAGAGGAGAAATACTAGATGGCTAACATCAAAAAGTTTGCAAAACAAGCA |
| 17we | sacB-UBP-New_gaF | 60 | nan | GTATAGTGAAAGAGCAGAAATACTAGATGGCTTAACATCAAAAAGTTTGCAAAACAAGCA |
| 17wf | retBB_sgRNA_gF | 64 | 65.0 | CGAAAAGCCTGCTCAACGAGCAGGCTTTTTTACTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC |
| 17wg | retBB_sgRNA_gR | 58 | 66.0 | GCTTAGTTGCATCTAGGTGACGCATCTCTCTCTCTAGAAGCGGCCGCGAATTCGAGTC |
| 17wh | mRFP-retBB_o2_f | 37 | 56.0 | CTTCGGTACTAGTGAAAGAGGAGAAATACTAGATGGC |
| 17wi | mRFP-retBB_o2_r | 31 | 57.0 | CCTATCATCGCTAGCACTGTACCTAGGACTG |
| 17wj | Split_pSB3C5_for | 26 | 59.0 | TGTATCACATATTCTGCTGACGCACC |
| 17wk | Split_pSB3C5_rev | 26 | 59.0 | GGTGCGTCAGCAGAATATGTGATACA |
| 17wl | Split_SpeI_for | 40 | 61.0 | TGCTAGCTGCTTGTGAAAGAGGAGAAATACTAGATGGCTT |
| 17wm | Split_SpeI_rev | 40 | 61.0 | TCCTCTTTCACAAGCAGCTAGCACTGTACCTAGGACTGAG |
| 17wn | lacUV5_Split_F | 65 | 55.0 | TATGCTTCCGGCTCGTATAATGGACAAAGAGGAGAAAGTAATGGATAAGAAATACTCAATAGGCT |
| 17wo | lacUV5_Split_R | 60 | 53.0 | CTCTTTGTCCATTATACGAGCCGGAAGCATAAAGTGTAAATATAAACGCAGAAAGGCCCA |
| 17wp | pSB1K3_Split_F | 24 | 59.0 | GTAAACTTGGTCTGACAGCTCGAG |
| 17wq | pSB1K3_Split_R | 24 | 59.0 | CTCGAGCTGTCAGACCAAGTTTAC |
| 17wr | lacNEU_F | 80 | 61.0 | TCGGCTCGTATAATGTGTGAAATTGTGAGCGGATAACAATTTCACACAAGGAGATTACTAGATGGATAAGAAATACTCAA |
| 17ws | lacNEU_R | 80 | 64.0 | GTGAAATTGTTATCCGCTCACAATTTCACACATTATACGAGCCGATGATTAATTGTCAACAGCCTGGGGTGCCTAAATTG |
| 17wt | lacNEU_BB_F | 46 | 67.0 | ACAATTTCACACAAGGAGATTACTAGTAGCGGCCGCTGCAGTCCGG |
| 17wu | lacNEU_BB_R | 53 | 69.0 | AATTCTCACGCTTTCCAGTCCTCTAGAAGCGGCCGCGAATTCCAGAAATCATC |
| 17wv | ColPCR_Mis_F | 22 | 55.0 | CCAGGCTGTTGACAATTAATCA |
| 17ww | ColPCR_R | 18 | 55.0 | CCTTCTACGAGCTGTCCG |
| 17wx | ColPCR2_F | 20 | 55.0 | CCGGGAGGCATTTACTAGAG |
| 17wy | c3_g1_i4_fw | 27 | 58.0 | CTTAAAGGTGTTGAACATGGTGAGATG |
| 17wz | c2_g1_i4_rv | 25 | 58.0 | ACCATGTTCAACACCTTTAAGCTTG |
| 17xa | c3g1i4_gBl_gF | 68 | 57.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCATTAGATCCTAAGGTTAAAGATC |
| 17xb | c3g1i4_gBl_gR | 58 | 57.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGCTCCCACTCAATGGTCGAAG |
| 17xc | pSB3C5_split_fwd | 20 | 56.0 | GTTTCGCCACCACTGATTTG |
| 17xd | pSB3C5_split_rev | 20 | 60.0 | ACGCTCAAATCAGTGGTGGC |
| 17xe | pSB3C5-SpeI-remo-gaF | 50 | 63.0 | TACAGTGCTAGCTCATAGTGAAAGAGGAGAAATACTAGATGGCTTCCTCC |
| 17xf | pSB3C5-SpeI-remo_gaR | 45 | 60.0 | TAGTATTTCTCCTCTTTCACTATGAGCTAGCACTGTACCTAGGAC |
| 17xg | sacB-Test-gaF | 43 | nan | GCGGCAGCCGCAGCAGCCGCAACCCGCGATACCAAAGATACCC |
| 17xh | mRFP-Test-gaR | 41 | nan | TGCGGCTGCTGCGGCTGCCGCAGCACCGGTGGAGTGACGAC |
| 17xi | sacB-test-gaF | 47 | nan | CGTTCGGACTTCACACGGCCAACATCAAAAAGTTTGCAAAACAAGCA |
| 17xj | sacB-test-gaR | 46 | nan | GGCCGTGTGAAGTCCGAACGACCACGCTCACGCTGCTATAAATTTC |
| 17xk | pTXB1_GMPSopt_f | 59 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCGCTGGATCCAAAAG |
| 17xl | pTXB1_GMPSopt_r | 61 | 59.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGTTCCCATTCGATTGTCGACGGAG |
| 17xm | pTXB1_CDAopt_f | 69 | 59.0 | TAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAACAATATCGCTTTGTAATTGAAGC |
| 17xn | pTXB1_CDAopt_r | 68 | 59.0 | CGCCCTCGGGTAGGGCAACTAGTGCATCTCCCGTGATGGCAGCTTTTGTTAGATGAACAATGAAACAC |
| 17xo | phyto_fw | 38 | 56.0 | AACGTCTCACTCGATCGATGCAAGCCAAACCATTTCAC |
| 17xp | phyto_rv | 53 | 54.0 | AACGTCTCACTCAATCGTCAATTGAAATTTAAATCTAATGTATCAATTGAATC |
| 17xq | GFP-NPtNTT2_gaF | 58 | nan | GCTCGTATAATGGACAAAGAGGAGAAAGTAATGCGTAAAGGAGAAGAACTTTTCACTG |
| 17xr | PtNTT2-NGFP_gaR | 49 | nan | AGTTCTTCTCCTTTACGCATTACTTTCTCCTCTTTGTCCATTATACGAG |
| 17xs | GFP-NPtNTT2_gaR | 64 | nan | GTCGGATACGGGCGTGCGGCTGCCGCTGCGGCTGCCGCTTTGTATAGTTCATCCATGCCATGTG |
| 17xt | PtNTT2-NGFP_gaF | 58 | nan | GATGAACTATACAAAGCGGCAGCCGCAGCGGCAGCCGCACGCCCGTATCCGACCATTG |
| 17xu | PtNTT2_seq2_fwd | 20 | nan | GGCCTGGTGTTTGTGTTTGG |
| 17xv | PtNTT2(31-575)-T7-gaF | 43 | nan | TAATACGACTCACTATAGGGAAAGAGGAGAAAGTAATGCTGCC |
| 17xw | PtNTT2-T7-gaF | 43 | nan | TAATACGACTCACTATAGGGAAAGAGGAGAAAGTAATGCGCCC |
| 7xx | PtNTT2-T7-gaR | 47 | nan | TCTCCTCTTTCCCTATAGTGAGTCGTATTACTCTAGAAGCGGCCGCG |
| 17xy | MHA-mRFP-seqR | 20 | nan | ACCTTCGTACGGACGACCTT |
| 17xz | CWH-mRFP_seqF | 20 | nan | CGGGTGCTTACAAAACCGAC |
| 17ya | CWH-sacB-seqR | 21 | nan | CGTGTAATATGGGAAATGCCG |
| 17yb | mRFPretBB_25_gF | 25 | 56.0 | GAAAGAGGAGAAATACTAGATGGCT |
| 17yc | mRFPretBB_25_gR | 21 | 57.0 | AGCACTGTACCTAGGACTGAG |
| 17yd | PtNTT2_histag_gaF | 48 | nan | CACCATCACCACCATCATCACCATTAATAATACTAGTAGCGGCCGCTG |
| 17ye | PtNTT2_histag_gaR | 55 | nan | ATGGTGATGATGGTGGTGATGGTGATGATGTTTTTCTTTGTTGGTTTTCGCTTCC |
| 17yf | Seq_Library_fw | 90 | 57.0 | AATGGGGTTAAAGGCAAAATATGTTTATGGAAGTGAATTCCAGCTTGATAAGGATTATACACTGAATGTCTATAGATTGGCTTTAAAAAC |
| 17yg | Seq_Library_rev | 81 | 58.0 | CTGCCCAGCTGGAGTTGTTGAAGGAAATCCAATAATGGAGATAGCTAAATACTTCCTTGAATATCCTTTAACCATAAAAAG |
| 17yh | ColPCR_PtNTT2_F | 21 | 55.0 | AAAATGGAAGCGAAAACCAAC |
| 17yi | split_kan_fw | 43 | nan | GTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTG |
| 17yj | split_kan_rv | 53 | nan | TCGTCTTGCAGTTCATTCAGGGCACCGGACAGGTCGGTCTTGACAAAAAGAAC |
| 17yk | split_kan_ggü_fw | 29 | nan | GGCGGTAATACGGTTATCCACAGAATCAG |
| 17yl | split_kan_ggü_rv | 29 | nan | CTGATTCTGTGGATAACCGTATTACCGCC |
| 17ym | Seq_57_fw_B8 | 89 | nan | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNCAGCTTGATAAGGATTATACACTGAATGTC |
| 17yn | Seq_58_fw_B11 | 92 | nan | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNGCTTGATAAGGATTATACACTGAATGTCTATAG |
| 17yo | Seq_57_fw_B21 | 89 | nan | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNGGATTATACACTGAATGTCTATAGATTGGC |
| 17yp | Seq_57_fwd_B3 | 90 | nan | AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNCACTGAATGTCTATAGATTGGCTTTAAAAAC |
| 17yq | Seq_58_B8_rev | 81 | nan | CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCACAACTCCAGCTGGGCAG |
| 17yr | Seq_57_alt_rev | 87 | nan | CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCNGATTTCCTTCAACAACTCCAGCT |
| 17ys | Seq_57_B11_rev | 91 | nan | CAAGCAGAAGACGGCATACGAGATGTAGCCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCCATTATTGGATTTCCTTCAACAACTC |
| 17yt | Seq_56_B21_rev | 93 | nan | CAAGCAGAAGACGGCATACGAGATCGAAACGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCGGATATTCAAGGAAGTATTTAGCTATCTCC |
