As proof-of-concept, we incorporated the non-canonical amino acid phenylalanine-4’-azobenzene (AzoF) into one enzyme coded by the gene crtI of the β-carotene pathway (BBa_K2201207) (Schaub et al., 2012). If the photoswitch is in the OFF state, the orange colored lycopene is not synthetized. After irradiation the photoswitch is turned ON, which leads to the synthesis of lycopene and its detection.
Photoswitching with Non-Canonical Amino Acids
- Photocaging: A “protection” group facilitates or inhibits the normal function of a given protein, but after cleaving of the chemical moiety the protein of interest is de/activated. This process is irreversible.
- Photoswitching: The used chemical moiety used can be switched between “ON" and "OFF” stages. This process is reversible.
For both approaches the incorporation of a chemical moiety into a permissive site of the protein of interest is accomplished through amber suppressor tRNA (Bose et al., 2006).
The advantage of light as the trigger for the cleaving and conformational change lies in its highly controllable, selective and inexpensive application. In contrast to chemical substrates used for induction of a reaction, light does not leave residues which themselves can influence the test environment. Furthermore, many already established techniques can be adapted to apply the specific wavelength and irradiation time for any possible non-canonical amino acid (Brieke et al., 2012).
- Name: Phenylalanine-4’-azobenzene
- Short: AzoF
- CAS: 154596-15-3
- MW: 269.299
- Storage: dark and cold
- Source: acccorporation
- Prize: 5mg - 498.88$
- Function: conformation is switchable by irradiation with light of specific wavelengths
Figure 1: Structure of AzoF in cis- and trans-conformation.
The universal precursors of the carotenoid biosynthesis pathway are the isomers isopentenyl pyrophosphate (IPP) and dimethylalkyl pyrophosphate (DMPP) (Rodríguez-Villalón et al., 2008). In nature, two independent pathways lead to the precursor biosynthesis: (i) The mevalonic acid (MVA) pathway, found in eukaryotes, archaea and some bacteria with the primary educt acetyl-CoA (Kirby and Keasling, 2009). (ii) The alternative metabolic methylery thriol phosphate (MEP) pathway, found in most bacteria and plant plastids with the primary educts being pyruvate and glycerylaldehyde-3-phosphate (GAP), which condensate (Rohmer, 1999; Lange et al., 2000; Rohdich et al., 2003).
IPP and DMPP are then catalyzed to farnesyl pyrophosphate (FPP) by two sequential prenyltransferase reactions by farnesyl diphosphate synthase encoded by ispA (Yuan et al., 2006). FPP is the common branch point for carotenoid, but also other isoprenoid biosyntheses like dolichols and quinons. Also FPP naturally occurs in the non-carotenogenic E. coli. For production of lycopene and β-carotene just four genes from the bacteria Pantoea ananas need to be heterologously expressed in E. coli (Choi et al., 2013; Yuan et al., 2006): (i) crtE coding for geranylgeranyl diphosphate synthase which catalyzes the condensation of FPP and IPP to form geranylgeranyl diphosphate (GGPP) (Misawa et al., 1990), (ii) crtB coding for the phytoene synthase which catalyzes the condensation of two GGPP to yield phytoene (Iwata-Reuyl et al., 2003), (iii) crtI coding for phytoene desaturase which forms lycopene (Fraser et al., 1992), and (iv) crtY coding for lycopene cyclase which catalyzes the cyclization of lycopene and thus converting it to β-carotene.
Just the two last products of the β-carotene biosynthesis are colored compounds with lycopene being red and β-carotene being orange due to their polyene chromophore (Schaub et al., 2012).
Figure 2: Biosynthesis Pathway of β-Carotene.
Two independent pathways feed the biosynthesis of β-carotene with the precursors IPP and DMAP. Based on Yoon et al., 2009.
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