We want our Carindex to be an easy-to-use product for the customers. So we apply our functional bacteria onto the test paper. By doing it, our test paper along with the bacteria can be preserved for a very long time.
Finally, these freeze-dried test paper will be placed between two pieces of glass slides and become the consumptive material of Carindex.
When customer want to use Carindex, the revived liquid (LB broth) will be dipped into the test paper between two glass slides to revive the functional bacteria. Once the bacteria have awake, the CSP and lactate level detecting functions will be activated. Hence, the bacteria will detect the concentrations of CSP and lactate in the sample.
Experiment and result of test paper
First of all, we cultured the bacteria in LB broth (Vtotal=5 ml) for 16 hours and add fixed proportion of glycerol (4.5%) and glucose (7.5%) into the it. Then, we dipped 50 µl of liquid culture onto the test paper and took it to the freeze dryer. After freeze-drying, we do the revived experiment to test our the bacteria survival.
After carrying out a series of experiments, we prove that bacteria will survive on the test paper after freeze-drying process. It would grow better if it is incubated after freezed drying. And one more thing to notice is that without adding glucose and glycerol, the survival of bacteria decreases drastically.