Purpose of this protocol is to assess the enzymatic activity of glycosidases using fluorophores conjugated to substrates of interest (such as cellobiose or xylobiose). Once the enzyme of interest cleaves substrate, the fluorophore is released enabling quantification of enzymatic activity.
Purpose of this protocol is to generate a library of cosmid clones that are representative of the microbiome of the porcupine for screening for novel enzymes.
To visual total protein in a given sample.
To identify specific amino acid sequences of a protein, or tag, using fluorescently-tagged antibodies.
Purpose of this protocol is to grow up single colonies of bacteria for use.
Purpose of this overview is to isolate and clone genes of interest into an appropriate plasmid and then introduce the recombinant DNA molecule into E. coli.
The purpose of this protocol is to assay carboxymethyl cellulose degradation via a pH change detectable by the dye Congo Red as differentially coloured halos around colonies of bacteria.
Concentration protocol to concentrate DNA isolating using the PowerFecal Extraction kit.
Purpose of this protocol is to create an agarose gel for visualizing and extracting DNA.
The purpose of this protocol is to create solid growth media containing cellobiose to measure beta-glucosidase activity.
Purpose of this protocol is to generate solid growth media containing LB and an antibiotic of choice for selective growth of successful clones following transformation.
Purpose of this protocol is to introduce plasmid DNA into E. coli cells.
The purpose of this protocol is to create solid growth media containing both carboxymethylcellulose and glucose for measuring endoglucanase activity.
Purpose of this protocol is to generate a salt solution for E. coli growth.