Team:Evry Paris-Saclay/Part
Parts
Basic Parts
During this project, our team designed the following basic BioBricks:
Coding sequences:
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448001 | mEmerald | yes | |
| BBa_K2448003 | LacZ-alpha (pUC19 like) | ||
| BBa_K2448004 | mCherry | yes | Yes of Part:BBa_J06504 |
| BBa_K2448006 | PsiR from Agrobacterium tumefaciens | ||
| BBa_K2448007 | PsiR from Sinorhizobium fredii | ||
| BBa_K2448008 | PsiR from Sinorhizobium meliloti | ||
| BBa_K2448009 | FruR from Escherichia coli | ||
| BBa_K2448021 | D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum | ||
| BBa_K2448022 | D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens |
Plasmid backbones:
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448036 | pSB1C3 BsmBI free | yes | Yes of Part:pSB1C3 |
| BBa_K2448037 | pSB3K3 BsaI free | yes | Yes of Part:pSB3K3 |
| BBa_K2448038 | pSB1C3 LacIq | yes | Yes of Part:pSB1C3 |
Ribosomal binding sites (RBS):
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448000 | B0034 with scars AGGA and AGATCA | ||
| BBa_K2448002 | B0034 like RBS with scars AATT and CAGCT | ||
| BBa_K2448020 | B0034 with scars TAC and CAT | ||
| BBa_K2448039 | B0034 with scars GTAC and TACCAT | ||
| BBa_K2448049 | B0034 with scars GTAC and CTCGAGg | ||
| BBa_K2448019 | RBS (pET like) |
Promoters:
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448016 | pPsiTac1 | Yes of Part:BBa_K864400 | |
| BBa_K2448050 | pPsiTac2 | Yes of Part:BBa_K864400 | |
| BBa_K2448010 | pPsiA from Agrobacterium tumefaciens | ||
| BBa_K2448011 | pPsiR from Agrobacterium tumefaciens | ||
| BBa_K2448012 | pPsiA from Sinorhizobium fredii | ||
| BBa_K2448013 | pPsiR from Sinorhizobium fredii | ||
| BBa_K2448014 | pPsiA from Sinorhizobium meliloti | ||
| BBa_K2448015 | pPsiR from Sinorhizobium meliloti | ||
| BBa_K2448017 | pFruB from Escherichia coli |
Terminators:
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448005 | B0015 with scars T and TGTT | ||
| BBa_K2448051 | B0015 with scars AGCGGCATGC and GCAGA | ||
| BBa_K2448018 | L3S1P51 terminator | ||
| BBa_K2448052 | L2U3H03 Terminator |
And various spacers in between the functional elements of our composite parts containing cloning sites :
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448040 | spacer with cloning sites XhoI and BsmBI | ||
| BBa_K2448041 | spacer with cloning sites BsmBI and SphI | ||
| BBa_K2448042 | spacer with cloning sites BamHI and BbsI | ||
| BBa_K2448043 | spacer with cloning sites BbsI and NcoI | ||
| BBa_K2448044 | spacer with cloning sites XhoI, BsmBI x2, SphI | ||
| BBa_K2448045 | spacer with cloning sites BamHI, BbsI x2, NcoI | ||
| BBa_K2448046 | spacer with cloning site SphI | ||
| BBa_K2448047 | spacer with cloning site BamHI | ||
| BBa_K2448048 | spacer with cloning site NcoI |
Composite Parts
During this project, our team designed the following composite BioBricks:
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448023 which is our Best Composite Part | Universal Biosensing Chassis (UBC) | yes | Part:BBa_K2448023 |
| BBa_K2448024 | Universal Biosensing Chassis (UBC) short version | yes | |
| BBa_K2448025 | Psicose Biosensor pPsiA-PsiR from Agrobacterium tumefaciens | yes | |
| BBa_K2448026 | Psicose Biosensor pPsiR-PsiR from Agrobacterium tumefaciens | yes | |
| BBa_K2448027 | Psicose Biosensor pPsiTac1-PsiR from Agrobacterium tumefaciens | yes | |
| BBa_K2448028 | Psicose-Biosensor pPsiA-PsiR from Sinorhizobium fredii | yes | |
| BBa_K2448029 | Psicose-Biosensor pPsiR-PsiR from Sinorhizobium fredii | yes | |
| BBa_K2448030 | Psicose-Biosensor pPsiA-PsiR from Sinorhizobium meliloti | yes | |
| BBa_K2448031 | Psicose-Biosensor pPsiR-PsiR from Sinorhizobium meliloti | yes | |
| BBa_K2448032 | Fructose Biosensor pFruB-FruR from Escherichia coli | yes | |
| BBa_K2448033 | D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum | yes | |
| BBa_K2448034 | D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens | yes | |
| BBa_K2448035 | Psicose Biosensor pPsiA-At PsiR-At with D-Psicose 3-epimerase from Clostridium cellulolyticum |
Part Collection
The Universal Biosensing Collection groups constructs derived from the Universal Biosensing Chassis (UBC) (BBa_K2448023 or BBa_K2448024). This UBC can be used to easily create biosensors able to sense and quantify virtually any compound by direct or indirect sensing. Designed for high throughput screening, thanks to Golden Gate Assembly, standardized fusion sites and insertion markers, it enables the characterization of many parts at once, coupled with a Fluorescence plate reader or Flow Cytometer. For instance we constructed, based on this UBC, different highly specific biosensors: a fructose biosensor and several psicose biosensors, each of them with a particular dynamic range and sensitivity. This collection could be the first step in a larger repository composed of standardized biosensors for a large number of molecules.
During this project, our team designed the first 8 members of the Universal Biosensing Collection:
| Part number | Name | Submitted to the registry | Improves the function of an existing BioBrick Part |
|---|---|---|---|
| BBa_K2448025 | Psicose Biosensor pPsiA-PsiR from Agrobacterium tumefaciens | yes | |
| BBa_K2448026 | Psicose Biosensor pPsiR-PsiR from Agrobacterium tumefaciens | yes | |
| BBa_K2448027 | Psicose Biosensor pPsiTac1-PsiR from Agrobacterium tumefaciens | yes | |
| BBa_K2448028 | Psicose-Biosensor pPsiA-PsiR from Sinorhizobium fredii | yes | |
| BBa_K2448029 | Psicose-Biosensor pPsiR-PsiR from Sinorhizobium fredii | yes | |
| BBa_K2448030 | Psicose-Biosensor pPsiA-PsiR from Sinorhizobium meliloti | yes | |
| BBa_K2448029 | Psicose-Biosensor pPsiR-PsiR from Sinorhizobium fredii | yes | |
| BBa_K2448030 | Psicose-Biosensor pPsiA-PsiR from Sinorhizobium meliloti | yes | |
| BBa_K2448031 | Psicose-Biosensor pPsiR-PsiR from Sinorhizobium meliloti | yes | |
| BBa_K2448032 | Fructose Biosensor pFruB-FruR from Escherichia coli | yes |
