Team:Fudan China/Parts

For this year’s iGEM competition, our team has chosen to submit three integrases CDS and their corresponding attB and attP sites. We have also submitted the attB and attP sites of phiC31 as improved parts. In our experiments, we tested all 5 integrases, phiBT1(BBa_K2460001), phiC31 and TG1(BBa_K2460007) from Steptomyces phage and Bxb1, phiRv1(BBa_K2460004) from Mycobacterium phage, for their effiency and orthogonality. Among these integrases, phiBT1 showed the best performance.

List of our submitted parts

Name Description

BBa_K2460001

 phiBT1 integrase

BBa_K2460002

 phiBT1 attB

BBa_K2460003

 phiBT1 attP

BBa_K2460004

 phiRv1 integrase

BBa_K2460005

 phiRv1 attB

BBa_K2460006

 phiRv1 attP

BBa_K2460007

 TG1 integrase

BBa_K2460008

 TG1 attB

BBa_K2460009

 TG1 attP

BBa_K2460010

 phiC31 attB

BBa_K2460011

 phiC31 attP