Team:Fudan China/Parts

For this year’s iGEM competition, our team has chosen to submit three integrases CDS and their corresponding attB and attP sites. We have also submitted the attB and attP sites of phiC31 as improved parts. In our experiments, we tested all 5 integrases, phiBT1(BBa_K2460001), phiC31 and TG1(BBa_K2460007) from Steptomyces phage and Bxb1, phiRv1(BBa_K2460004) from Mycobacterium phage, for their effiency and orthogonality. Among these integrases, phiBT1 showed the best performance.

List of our submitted parts

Name Description

BBa_K2460001

phiBT1 integrase

BBa_K2460002

phiBT1 attB

BBa_K2460003

phiBT1 attP

BBa_K2460004

phiRv1 integrase

BBa_K2460005

phiRv1 attB

BBa_K2460006

phiRv1 attP

BBa_K2460007

TG1 integrase

BBa_K2460008

TG1 attB

BBa_K2460009

TG1 attP

BBa_K2460010

phiC31 attB

BBa_K2460011

phiC31 attP

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