Have you already chosen a protein to be evolved?
You already accomplished the first and most important step. Let’s see whether
you are ready to evolve it!
No problem, there are lots of proteins waiting to be evolved. Go on to find
out what you should take care of when choosing your target protein and preparing your experiments.
Maybe you also want to have a look at our different projects
to get an idea of the wide range of proteins you can evolve with PREDCEL
Which application will your evolved protein be used for?
Did you discuss your idea with a medical professional? We are sure this expertise
would be valuable to choose the right experimental settings.
Did you discuss your idea with a chemist? This expertise could be helpful to figure out how to set up the evolution efficiently, as parameters such as passive diffusion of the chemical through the E. coli cell wall, toxicity and many others needs to be carefully considered.
Be aware that a number of countries have strict regulations regarding green biotechnology.
That sounds quite uncertain. Before you start or pursue evolving your protein,
you should know the exact purpose and impact. How about engaging with your
local community to figure out possible needs?
Engage with the community in the field of in vivo evolution – we´d be interested
in discussing your project.
Are you sure your protein is safe to be evolved?
Nevertheless we advise you to check it in our SafetyNet, there could be “sleeping”
Please, check your protein sequence for any “sleeping” hazardous potential in our
Do you know which equipment is required to safely work with phages?
Did your considerations include that phages are very volatile? You should create
a special “phage space” in your lab to reduce contamination. Furthermore,
you should comply with the safety level of your evolving protein. Check the
safety level of your protein on our SafetyNet
Check oursafety page
for general advice and get in touch with your safety representative for detailed discussion of the particular work you plan.
Before starting any phage work, please consult our safety page
. Also, we advise you to talk to your colleagues next door with experience in phage work as well as your local safety representative.
Did you calculate all required parameters on our Integrated Modeling page?
Well done! Now you have the optimal starting conditions for your experiment.
Visit our Integrated Modeling page to figure out all required parameters for a successful PREDCEL run.
You should consider that our Integrated Modeling reduces effort, costs and environmental burden by enabling in silico optimization of experimental conditions.
Did you already engage with your local community to discuss your plans?
You should reach out and listen to the feedback, hopes and concerns of your local community. Thereby, you can facilitate a positive, beneficial impact of your research project.
Are you sure? We encourage you to do so and be surprised, how enriching it is to discuss your research plans with people inside and outside the research community. Also consider, that your research may very well impact the people around you and hence, they should have a word and vote in what our technologies are used for. Finally, showing that you are a responsible scientists caring about the consequences of your research will surely have a positive impact on your reputation.
Did you consider possible consequences of your planned PREDCEL experiment?
You are a responsible scientist. We encourage you to use our PREDCEL toolbox and convey the iGEM spirit of responsible science to your friends and colleagues.
Take your time to reflect on possible consequences to prevent unintended dangers. This is an essential step to be taken before starting directed evolution experiments.
Are you sure? We encourage you to talk to experts and your local community. You will be surprised how much they will appreciate your offer to discuss the consequences of your research BEFORE implementing it in the lab. Also, consider the positive impact for yourself: Showing that you are a responsible scientists caring about the consequences of your research will surely have a positive impact on your reputation.
Now it´s on you!
When you talk about PREDCEL with your colleagues, friends and local communities, you can spread the idea of making directed evolution experiments safe and to use this technology responsibly for addressing urgent human needs.