In our design, we employ luxR gene that forms a transcriptionally active complex with 3OC6HSL to activate a downstream construct. Once activated, the desired functions of the circuit will initiate a "time bomb" that terminates this synthetic process.
After a period of time (which varies depending on the application), termination of function will be accomplished by knocking out the genes of interest using Cre-lox and/or Dre-rox. As a result, the entire local population of genetically modified organisms can be reverted back to its wild-type phenotype, with or without getting exterminated to prevent sudden and drastic changes in the ecosystem.
Watch our video for a general understanding first !!!