Team:IISER-Pune-India/Composite Parts

Composite parts
  • pCAT-RBS-AmilCP-Double Terminator; BBa_K228003 This composite part constitutively expresses amilCP under the control of E.coli pCAT promoter. High efficiency ribosome binding site Bba_B0034 and double terminator Bba_B0015 are also integrated to this construct.
    Ref: Diversity and Evolution of Coral Fluorescent Proteins,Naila O. Alieva, Karen A. Konzen, Steven F. Field, Ella A. Meleshkevitch, Marguerite E. Hunt, Victor Beltran-Ramirez, David J. Miller, Jörg Wiedenmann, Anya Salih, Mikhail V. Matz
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  • pL-pLeft promoter-RBS-AmilCP-Double Terminator; BBa_K228004 : This composite part constitutively expresses amilCP under the control of hybrid promoter pL-pLeft. High efficiency ribosome binding site Bba_B0034 and double terminator Bba_B0015 are also integrated to this construct. This part expresses AmilCP in E.coli since pL promoter expresses constitutively in E.coli.
    Ref: 1.PL Expression System : A Prokaryotic Expression System, Invitrogen manual 2.Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU 3.Diversity and Evolution of Coral Fluorescent Proteins,Naila O. Alieva, Karen A. Konzen, Steven F. Field, Ella A. Meleshkevitch, Marguerite E. Hunt, Victor Beltran-Ramirez, David J. Miller, Jörg Wiedenmann, Anya Salih, Mikhail V. Matz

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