No work was conducted with Risk Group 3 or 4 organisms.
Chassis organism used : E.coli.
Strains of E.coli used : DH5-alpha, K-12, BL-21.
Description : Risk Group 1 organisms do not cause disease in healthy adult humans.
Lab Safety Level : Safety Level 1.
Besides our chassis, we used Streptomyces griseus (Orf2), Bacillus megaterium, Pseudomonas putida(SrpR) , Pseudomonas fluorescens (PhlF) and Bacillus subtilis (LmrA) because we are interested in repressor proteins expressed in these micro-organism. We have found the sequence and had it synthesized by a provider or fish it out from the organisms using PCR.
Since The project is not associated with Risk Group 3 and 4 Organisms and therefore no work was conducted by the team in a Safety Level 3 or 4 laboratory.