After visiting physicians and villages with people affected by T. solium, Dimi and Henrik went to IIT Delhi to establish RNA isolation in the lab of iGEM IIT Delhi. Having this option to do RNA isolation was crucial for diagnostX because laboratories in Varanasi did not have the equipment to isolate RNA and there was a realistic chance that isolation of RNA was necessary for export. The team from Delhi found a student dorm for Henrik and Dimi to stay in and thus we could work day and night to optimize an RNA isolation protocol that ultimately yielded high-quality RNA.
While we were working on our square wave generator project, one of the essential things was to remove the Degradation tags in order to reduce noise. But the parts we got from the kit plates had Degradation Tags attached to them already. So, in order to remove them, we decided to use knockout strains. CLPP Protease is a strain which recognizes the degradation tags and degrades them, but it wasn’t available to us in our lab. This is when iGEM Berlin Team helped us out and they sent us the strains as they were available in the Berlin diagnostX lab.
We would like to thank and congratulate the iGEM Berlin diagnostX team on this successful collaboration and look forward to doing more work together in the future.