Team:ITB Indonesia/Part Collection


Parts

Parts Collection


Parts Collection

This is the parts collection designed by ITB Indonesia iGEM Team 2017.

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Part Name BioBrick Description Part Type
PETase BBa_K2378001 This is optimized coding sequence of PETase enzyme for E. coli BL21. Coding
IrrE Coding Sequence BBa_K2378007 This is improved coding sequence of IrrE from BBa_K729001 (http://parts.igem.org/Part:BBa_K729001). When transformed to E. coli, this protein protected from salt, oxidative, and thermal shock. Coding
EndA Endonuclease Coding Sequence from Vibrio cholera BBa_K2378008 This endonuclease degrades free extracellular DNA. The gene is isolated from Vibrio cholera. Coding
pSal + PETase Coding Device BBa_K2378004 ITB_Indonesia iGEM team 2017 designed a PETase enzyme coding device consisting of NahR-pSal regulatory part (BBa_J61051, http://parts.igem.org/Part:BBa_J61051) which is activated by salicylate inducer. In effect, PETase is only expressed when this device detect the presence of salicylate, which binds to NahR. Composite
Constitutive PETase Coding Device BBa_K2378005 This is optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of constitutive promoter BBa_J23106. Composite
NhaR-PETase Constitutive Coding Device BBa_K2378006 This is an optimized coding sequence of PETase enzyme for E. coli BL21 with the addition of NhaR BBa_K1357002 (http://parts.igem.org/wiki/index.php?title=Part:BBa_K1357002) coding sequence. NhaR enhances the transcription of pgaABCD operon which in turn induces the production of biofilm. This part also uses constitutive promoters BBa_J23106. Composite
EndA Endonuclease Coding Device from Vibrio cholera BBa_K2378009 This is coding device of endonuclease degrades free extracellular DNA, with constitutive promoter BBa_J61051. The gene is isolated from Vibrio cholera. Composite

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