- dCAS13-a enzyme is modelled as a sphere
- The mRNA in the cytoplasm is stationary.
- The dCas enzyme faces no other competition from other organelles or objects inside the cell.
- All conditions in the cell is kept constant.
- When the dead dCas13 binds to a mRNA molecule it stays bound to it.
- Size of fibroblast is the actual size minus the nucleus.