Team:Lethbridge HS/Contribution





 Basic Parts



3gt

The gene 3gt is from the anthocyanin synthesis pathway and converts the initial molecule Pelargonidin into Anthocyanin. This gene is from the organism Petunia hybrid. We have added this parts sequence to the registry for use in our composite part BBa_K2481105. The basic part is BBa_K2481002

yadH

This Gene is an Escherichia coli gene that has been shown to increase the yields of anthocyanin when paired with the genes in our anthocyanin construct. We have added the sequence to the registry for use in our composite part BBa_K2481105. The basic part is BBa_K2481004

f3h

We will be useing the gene f3h as the first gene in our anthocyanin synthesis pathway, it comes from the organism Petroselinum crispum. We have added this sequence to the registry as part BBa_K2481111. This gene will code for a protein that converts the initial molecule flavanone into dihydroflavonol.

dfr

The gene dfr is the second one in our anthocyanin synthesis pathway. We are using the biobrick part BBa_K2481110. We have added the sequence to the registry as our gene is from a different organism than the existing part in the registry.

ans

This gene is the third gene in our pathway, it converts the molecule created by dfr into pelargonidin. It is from the organism Malus domestica and we have added it to the registry. It is an engineered anthocyanidin synthase. Part BBa_K2481112.

indB

This gene is from the organism Streptomyces chrmofuscus, and it is our original basic part submission to the registry. Its is part BBa_K2481001. It has been shown to increase the yeilds of Indigoidine when used with indC. It is a putative phosphatase.



 Composite Parts



 Melanin Construct

Melanin composite part

BBa_K2481108
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
melA Part BBa_K193600.
Terminator Part BBa_B0015.

This construct allows us to make Melanin out of L-Tyrosine.


Figure 1. melA expression construct for use in E. coli BL21(DE3).

 Anthocyanin Constructs


Anthocyanin composite part 1

BBa_K2481113
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
dfr Part BBa_K2481110
f3h Part BBa_K2481111
Terminator Part BBa_B0015.


Anthocyanin composite part 2

BBa_K2481114
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
ans Part BBa_K2481112
Terminator Part BBa_B0015.


Anthocyanin composite part 3

BBa_K2481105
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
3gt Part BBa_K2481002
yadH Part BBa_K2481004
Terminator Part BBa_B0015.

These composite parts come together to complete the pathway from Eriodictyol to Anthocyanin. These constructs are separated due to the size of the genes. It would be too much of a sstrain on the cell to have all of th genes in one or even two plasmids and this is why we needed three separate constructs. These constructs are each submitted





 Zeaxanthin Construct

Zeaxanthin composite part

BBa_K2481107
T7 Promoter Part BBa_I712074.
RBS Part BBa_B0034.
crtY Part BBa_I742154.
crtZ Part BBa_I742157.
Terminator Part BBa_B0015.

This construct is in the plasmid psB1C3, and will convert our initial molecule Lycopene into our final product, the pigment Zeaxanthin.



 Indigoidine Constructs

Indigoidine composite part 1

BBa_K2481106
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indB Part BBa_K2481001
Terminator Part BBa_B0015.


Indigoidine composite part 2

BBa_K2481109
T7 Promoter PartBBa_I712074.
RBS Part BBa_B0034.
indC Part BBa_K1152013
Terminator Part BBa_B0015.

These two composite parts come together to convert our initial molecule Glutamine into Indigoidine. The indB has been shown to increase the yields of Indigoidine as well as it is our original basic part submissions. We had to split our genes into two separate composite part submissions as the size of each was too large to allow for them to be in one plasmid.











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