Team:MIT/model

• ABOUT US
  Team Attributions
• BACKGROUND
  Alternative Splicing CRISPR RNA Binding Proteins REST
• PROJECT
  Guides and ASOs mKate-FF4 2 Exon mKate HBG 3 Exon mKate HBG 3 Exon Dual Fluorescence
• LAB WORK
  Parts/Improved Parts Protocols Notebook Future Work
• MODELLING
• HUMAN PRACTICES
  Integrated HP Public Engagement Collaborations InterLab
• AWARDS
  HP Silver HP Gold Education & Public Engagement Model Parts Collection

Splice MIT

Overview

SpliceMIT is a tool to generate antisense oligonucleotides (ASO) for a given DNA/RNA sequence, and then analyze and output the most effective ASOs. This tool could be used for a variety of scenarios. For our test samples, SpliceMIT was used to generate and analyze the gRNA sequences (complementary to the region on the intron) in the CRISPR – dCas13a system to control alternative splicing by targeting mRNA sequences.