To show the speed and usage simplicity of CascAID, we recorded the setup of our detection reaction on paper using Lightbringer to detect the fluorescence produced by the RNaseAlert system. In less than half an hour you can see the increase in fluorescence and determine if pathogens were present in the sample.
With our modular approach, we prototype all units of our diagnostic device, and integrate them into a customizable platform. We use cell-free synthetic biology to distinguish between pathogens with our universal detection cascade.
Sensitive and Rapid
Robust and Universal
Portable and low-cost
We chose to combine heat lysis and isothermal amplification (RPA) to extract our target RNA from patient samples.