Team:Nanjing-China/Improvement
In order to further extend the range and improve the sensitivity of detection, we expect to enhance the expression of SQR by substituting psqrR with pcons, the most efficient promotor among the igem parts. By doing so, we ensure that there is enough SQR in the system to oxidize even a small amount of S2- in time.
We have replaced psqrR with BBa_J23100(pcons). Part BBa_J23100 can successfully start the expression of sqr. After the replacement, we have observed RFP response upon inducing the new pathway with 100 μmol/L Na2S, which proves that the new pathway works.We are still measuring the strength of pcons against psqrR in the function Part BBa_K2257006.
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Dual-enzyme digestion of pcons recombinant cells(left) and psqrR recombinant cells(right). | |
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Fig. 3a) RFP responsiveness of the second detector system in which the promotor of sqr was replaced with pcons (BBa J23100). | |
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Fig. 3b) A visible photograph of a)
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