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June 1st
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June 2nd
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June 3rd
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June 4th
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June 5th
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June 6th
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June 7th
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June 8th
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June 9th
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June 10th
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June 11th
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June 12th
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June 13th
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June 14th
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June 15th
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June 16th
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June 17th
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June 18th
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June 19th
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June 20th
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June 21st
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June 22nd
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June 23rd
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June 24th
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June 25th
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June 26th
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June 27th
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June 28th
- Jess + Marcia
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Overnight cultures made
5 ml LB
Ampicillin-100µg/ml
Stock-20 mg/ml
C1V1=C2C2
V1x20 000=100x5 V1=500/20 000 V1=0.025ml=25µl
Cam - 12.5 µg/ml
Stock - 2.5 mg/ml
V1 x 2500 = 2500 12,5 x 5
V1 = 62.5/2 500
V1 = 0.025 ml = 25 µl
June 29th
- Description
- Lais + Marcia + Ansh
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Miniprep carried out according to Qaigen miniprep kit protocol
Conc (ng/µl) Trial 2 Conc (ng/µl)
SGFP 248.8
J5100 179.1
K516030 78.7 113.2
K1758500 139.5
B0534 80.7 90.4
J33201 128.9
J04500 172.2
K143012 91.1 105.4
I70468 143.5
B0010 199.9
C0171 99.4 106.4
June 30th
- Declan + Lais
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Digest prep -
16 µl sample
2 µl buffer
1 µl PST1
1 µl EcoRI
Digest at 37C for 1 hour
Agarose gel prepped - 0.8% gel = 0.8 g in 1000 ml of TA bugger (1M)
Dye calculation - 20 µl in PCR tubes, dye needs to be 6x
Digested - so 20/6 = 3.666 µl in each
Undigested - 6/6 = 1 µl
Gel plan -
Lanes
1 - 100 bp ladder
2 - I7468
3 - B0534
4 - K516030
5 - C0171
6 - K143012
7 - J5100
8 - J04500
9 - B0010
10 - K1758500
11 - J33001
12 - 1 kb ladder