In the realm of synthetic biology, plate readers are useful tools that are found in laboratories all over the world as they allow for fast, reliable data acquisition over a large sample size.
However, due to this excessive cost, many potential consumers around the world are unable to use these machines or settle for using simple cuvette reading devices without a lot of functionality like those supplied by Vernier Software and Technology.
Our team decided to tackle this problem and create a device that offers the functionality and efficiency of plate readers for a much lower price. As a result, we designed KAM-Spec, an open-source, cost-effective and dispersion-based microplate reader capable of full visible spectrum absorbance and fluorescence measurements.
We tested the functionality of KAM-Spec by performing a series of standard synthetic biology experiments with our device.
We show that KAM-Spec is capable of the following:
(1) Resolving full absorbance & fluorescence spectra over the visible light range
(2) Measuring the dynamic change in a spectrum over time due to a stimulus
(3) Resolving a dose response curve