Team:SJTU-BioX-Shanghai/Signal Pathway-cn
信号通路
信号通路模型
我们的模型帮助描述了多因子可视化检测系统的细胞内反应。我们也有它来分析和评估我们的合成生物学系统。模型在Simbiology，MATLAB的工具箱下完成。
从图表中可以看出，我们找到了几个典型的变量变化的模式。实验结果与我们的模型吻合的很好。
通路图解
Figure1: Signal Pathway constructInducers enters the cell, activates receptor and binding with repressor protein, leading to antisense producing. Then antisense binds with STAR. Finally sfGFP expression starts.
反应
模拟结果
Figure2: sfGFP degradation over time.
Figure3: antiSTAR1 variation over time.
Figure4: STAR & STAR_complex variation over time.
Figure5: sfGFP& STAR_complex variation over time.
从这些图中，我们发现几个典型的变量变化的模式。实验结果与模型吻合的很好。
参数
| Parameters | Reaction | Value | Reaction Type |
| maxVproduceAsRprotein | AsrR + RNA_polymerase -> AsrR + RNA_polymerase + AsrR_protein | 0.6 | MassAction |
| RNApolyConstant | All Reaction with Polymerase | 1 | |
| kr_AsR_complex | As + AsrR_protein <-> AsR_complex | 0.8 | MassAction |
| kf_AsR_complex | As + AsrR_protein <-> AsR_complex | 0.05 | MassAction |
| maxVproduceanti | RNA_polymerase + Promoter_STAR1 -> RNA_polymerase + Promoter_STAR1 + antiSTAR1 | 60 | Competitive-Inhibition |
| AsrRproteinRepressConstant | RNA_polymerase + Promoter_STAR1 -> RNA_polymerase + Promoter_STAR1 + antiSTAR1 | 10 | Competitive-Inhibition |
| kf_STAR1complex | antiSTAR1 + STAR1 <-> STAR1_complex | 50 | MassAction |
| kr_STAR1complex | antiSTAR1 + STAR1 <-> STAR1_complex | 0.8 | MassAction |
| maxVproducesfGFP | Promoter_target + RNA_polymerase -> Promoter_target + RNA_polymerase + sfGFP | 100 | Competitive-Inhibition |
| antiRepressConstant | Promoter_target + RNA_polymerase -> Promoter_target + RNA_polymerase + sfGFP | 10 | Competitive-Inhibition |
| kd_AsrR | AsrR -> null | 0.007 | MassAction |
| kd_RNA_polymerase | RNA_polymerase -> null | 0.007 | MassAction |
| kd_AsrR_protein | AsrR_protein -> null | 0.007 | MassAction |
| kd_antiSTAR | antiSTAR1 -> null | 0.007 | MassAction |
| kd_STAR | STAR1 -> null | 0.007 | MassAction |
| kd_STARcomplex | STAR1_complex -> null | 0.007 | MassAction |
| kd_sfGFP | sfGFP -> null | 0.007 | MassAction |
Table1
变量
| Species | Description | Initial Value |
| AsrR | As repressor sequence | 100 |
| RNA_polymerase | RNA polymerase | 2000 |
| antiSTAR | antiSTAR sequence which binds with STAR to promote sfGFP | 0 |
| STAR | Small Transcription Activated RNA | 200 |
| STAR_complex | Complex of STAR&antiSTAR | 100 |
| sfGFP | Fluorescence Protein which displays green color | 541.5 |
| AsrR_protein | As repressor protein | 200 |
| As | As as an inducer | 1000 |
| AsR_complex | Complex of AsrR_protein&Promoter_STAR | 0 |
| Promoter_STAR | Promoter which generates antiSTAR sequence | 60 |
| Promoter_target | Promoter which is repressed by STAR | 80 |
Table2
