Team:SZU-China/Parts

PARTS

Basic Parts

This year, our team ultilizes 2 kinds of new parts: one is for carbonic anhydrase expression, another is for Na⁺-H⁺ antiporter, which can help improve alkaline resistance.You can click on the part number to see the details.

Part Number	Name	Type	Part Description
BBa_K2232000	TSLV1-CA	Basic	Coding sequence for Carbonic Anhydrase α from Bacillus Subtilus TSLV1
BBa_K2232005	OF4-CA	Basic	Coding sequence for Carbonic Anhydrase α from Bacillus Subtilus OF4
BBa_K2232001	OF4-nhaC	Basic	Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus OF4
BBa_K2232002	C125-nhaC	Basic	Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus C125
BBa_K2232007	BS168-nhaC	Basic	Coding sequence for Na+ - H+ antiporter nhaC from Bacillus Subtilus 168
BBa_K2232003	C125-tupA	Basic	Coding sequence for another Na+ - H+ antiporter TupA from Bacillus Subtilus C125

Composite Parts

The 2 kinds of basic parts described above is assembled together with promoter, ribosome binding site or terminator, which are presented in the following table. You can click on the part number to see the details.

Part Number	Name	Type	Part Description
BBa_K2232011	RBS-C125-tupA-T1	Composite	Encoded by the gene tupA from the facultative alkaliphilic strain Bacillus lentus C-125
BBa_K2232012	P43-OF4-nhaC-T1	Composite	The gene nhaC from the Bacillus pseudofirmus OF4 encodes the Na+/H+ antiporter
BBa_K2232013	P43-nhaC-T1	Composite	This part is the Na+/H+ antiporter coding sequence (CDS) from the B. subtilis strain 168
BBa_K2232014	P43-TSLV1-CA	Composite	Coding region of the pink mutant version of amilCP
BBa_K2232015	P43-OF4-CA	Composite	Coding region of the purple mutant version of amilCP
BBa_K2232026	P43-CA-β	Composite	Coding region of the green mutant version of amilCP

Improved Parts

This year, our team have improved the existing parts by either chracterization or optimize their sequence. The improved parts (in black) and original existing parts (in grey) are presented below. You can click on the part number to see the details.

Encoded by the gene tupA from the facultative alkaliphilic strain Bacillus lentus C-125

Part Number	Name	Type	Part Description	Improvement
BBa_K2232020 BBa_K911008	PsspB-gerAa	Composite	Germination-promoting receptor (gerA) expression system	Optimize its sequence
BBa_K2232022 BBa_K2465205	RBS-CsoS3-T1	Composite	Carbonic anhydrase (csoS3) expression system	Optimize its sequence
BBa_K2232025 BBa_K302035	Sucrose-limitation induced kill switch	Composite	Coding region of Sucrose-limitation induced kill switch	Characterize in Bacillus Subtilus