Team:Stockholm/Attributions

Attributions

Unless otherwise stated, all the experiments, constructs, code, wiki and results documented on our wiki were performed, written and collected by us.

General Support

Supervisors

Dr Johan Rockberg, primary iGEM PI - Leads a research group at the department of Proteomics and nanotechnology at KTH Royal Institute of Technology, provided lab space and advised us on how to progress in the lab.

Dr Ute Rölming, secondary iGEM PI - Leads a research group at the department of Microbiology and Tumor and Cell Biology at Karolinska Institutet, provided lab space and advised us on how to progress in the lab.

Advisors

Dr Thomas Crouzier - Group leader in the Division of Glycoscience at KTH Royal Institute of Technology, was one of our advisors helping in the project developement, enzyme testing and rheology testing. Additionally he provided all samples related to mucus and provided lab space.

Dr My Hedhammar - Leads a research group at the department of Proteomics and nanotechnology at KTH Royal Institute of Technology, gave instructions on how to solve problems faced in the lab. Was of great help when interpreting SDS-PAGE results.

Aman Mebrahtu - Team member of iGEM Stockholm 2016, provided everyday guidance and support in the lab, as well as multiple troubleshooting sessions.

Max Karlander - Team member of iGEM Stockholm 2015, provided everyday guidance and support in the lab, as well as multiple troubleshooting sessions.

Giulio Bernardinelli - Team member of iGEM Trieste 2011, provided project development support, difficult techniques support, sequence design and synthetic biology seminars, daily troubleshooting and interpretation of results. Also gave scientific advice and support for the future scenario video. Being a great help and inspiration.

Gustav Notander - A technology transfer manager at KTH Innovation, helped with the sponsoring strategy and design.

Maria Malmrud - Educational Coordinator at the Stockholm School of Entrepreneurship, proofread the texts for Human Practices and Entrepreneurship for our wiki.

MD Robert P. Dickson - Assistant Professor at the department of Pulmonary and Critical Care Medicine at University of Michigan, advised on project development by helping to understand the impact of mucus targeted treatments.

Dr Ryan C. Hunter - Assistant Professor at the Department of Microbiology and Immunology at University of Minnesota, advised on project development by helping to understand the lung microbiota function in relation to mucus-based lung disease.

Dr Thierry Hennet - Vice Dean for Studies at the Faculty of Science at the University of Zurich, advised on project development by discussing the application of mucus degrading enzymes.

MD. Michael Runold - Chief physician at the department of Lungs and Allergies at Karolinska Institutet, advised on project development and helped define the scope of the project.

Lab support

Dr Hongji Yan - Postdoc in Dr. Thomas Crouzier Group, helped in the enzyme activity testing and generally supported our lab work.

Gunaratna Kuttuva Rajarao - responsible for the BSL-I lab at KTH Royal Institute of Technology, did the safety training and provided help in safety related issues.

Sarah Wideman - iGEM Stockholm 2015 member, provided protocols for osmotic pressure experiments and advised on the experimental setup.

Linnea Österberg - iGEM Stockholm 2015 member, provided protocols for osmotic pressure experiments and advised on the experimental setup.

Sulman Shafeeq - Postdoc in Dr. Ute Römling Group, provided general lab support and did safety training at the BSL-II lab at Karolinska Institutet

Dr Søren Christensen - Principal scientist at the pharmaceutical company Lundbeck, provided a plasmid for the sialidase.

Dr Yu-Teh Li - Professor in the Department of Biochemistry and Molecular Biology at Tulane University, provided a plasmid for endo-β-galactosidase.

M.D. James M. Fleckenstein - Associate professor in the Departments of Medicine, Molecular Microbiology at Washington University School of Medicine in St. Louis, provided a plasmid for metalloprotease.

Dr Olivier Berteau - From the French National Institute for Agricultural Research, provided a plasmid for sulfatase.

Herbert Schweizer - provided pTNS2 transposase complex plasmid (Addgene plasmid #64968)

Difficult technique support

Dr Francisco Vilaplana - Group leader in the Division of Glycoscience at KTH Royal Institute of Technology, performed HPAEC analysis of our samples enzyme activity samples.

Dr Amparo Jimenez Quero - Postdoc student in Francisco Vilaplana Group at KTH Royal Institute of Technology, who performed HPAEC analysis of our samples and helped with experiment design and result interpretation.

Dr Antonio Martinez Abad - Postdoc student in Francisco Vilaplana Group at KTH, who performed HPAEC analysis of our samples.

Daniel Hult - PhD student at School of Chemical Science and Engineering at KTH Royal Institute of Technology, who helped test and handle the rheometer for rheology measurement of our mucus samples

Peter Hodder - Senior Application Scientist Rheology at TA Instruments, who provided the 40mm plate (free of charge) for the rheology testing, who advised us on the usage of the suitable rheology equipment and gave feedback on data interpretation.

Human Practices support

Gustaf Edman - iGEM judge, helped us center our human practices and inspired us to write the Human Practices Handbook.

Rosa Lönneborg - Patent search and critical information literacy teacher at KTH Royal Institute of Technology, helped in the development of our patent strategy and conducted a patent search for us.

Dr Erik Forsse - Senior advisor at the Director’s Office at Karolinska Institutet, helped by advising on our policy for recombinant LBPs that is part of our Integrated Human Practices.

Andreas Lundquist - from the Unit of Bioentrepreneurship at Karolinska Institutet, helped by advising on our entrepreneurship texts for our wiki and by developing our final product design.

Katarina Mercer - Senior Advisor for Clinical Development & Regulatory Affairs, helped by conducting an initial search in the regulation that could be relevant for us and by formulating our regulatory strategy.

Wiki support

Jakob Dahlström - Photographer, took our team and personal photographs for the wiki.

Cajsa Jönsson - Actress, performed voice over work for our project videos.

Acknowledgements

Thanks and acknowledgements for all other people involved in helping make a successful iGEM team.

General support: Lars-Arne Haldosén, Maria Masucci, Jan-Olov Höög, Emma Hääg, Megan Osler, Medicinska Föreningen.

Finance sponsors: Karolinska Institutet, KTH Royal Institute of Technology, BioGaia, Mölnlycke Healthcare, Johnson and Johnson, Sigma Aldrich, Folkuniversitetet, MathWorks, Atlas Antibodies, New England Biolabs, IDT, GATC, SNE, Nespresso Professional, Eppendorf, Red Bull, TA Instruments, World Scientific Publishing, Stockholm Makerspace.

Human Practice supporters: Andreas Lundquist, Gustav Notander, Maria Malmrud, Erik Forsse, Marko Ahteensuu, Rosa Lönneborg, Katarina Mercer, Gustaf Edman, Michael Runold, Niclas Johansson, Annika Hollsing, Robert Dickson, Pernilla Sönnerfors, Ulrica Sterky, Patrik Hidefjäll, Mattia Tettamanti.

Research support: Oscar He, Torbjörn Gräslund

Team building: FFF Stockholm

Sponsors