Team:Tongji China/Results


Tongji iGEM - Results
Tongji iGEM
TongJi iGEM
Results
The outcomes of our experiments.
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Plasmid Construction

We constructed three plasmid successfully: pUAST-pleP-GAL4, pUAST-pleP-GAL80ts and pUAST-UAS-TH.
This is our digest verification map:

1% gel electrophoresis of plasmid
pUAST-pleP-GAL4
1% gel electrophoresis of plasmid
pUAST-pleP-GAL80ts
1% gel electrophoresis of plasmid
pUAST-UAS-TH

Behavior Tests

Experiment 1: Use real-time PCR to determine whether the expression of TH is higher at 29°C

TH expression
Figure 1 - The relative expression of TH in modified fruit flies increased significantly.

Experiment 2: Detect male-male courtship when raising the temperature

Mating index (the relative time that the fruit fly use for mating):
Mating Index
Figure 2 - The mating index of the treated group rises significantly in at 29°C.
[time=5minutes, n=5, P<0.01]

Experiment 3: Test the modified Drosophila’s gender preferences

Comparison of time that the fruit flies use for mating male or female flies:
male-male and male-female mating
Figure 3 - The modified fruit flies had no preference for males and females when mating.
[time=10minutes, n=8, P>0.5]

Experiment 4: Test whether the modified fruit flies increase their appeal for males

The time that the fruit fly use for mating in five minutes:
There was no courtship in all groups.
[time=5minutes, n=5]

Experiment 5: Does the co-culture of wild-type and modified fruit flies influence their courtship with wild-type females?

Comparison of time that the fruit flies use for mating male or female flies:
male-male and male-female mating
Figure 4 - The co-culture of wild-type and modified fruit flies did not affect the courtship of wild-type flies.
[time=5minutes, n=5, P>0.5]

Experiment 6: Is the viability of modified fruit flies affected?

The number of live flies in the absence of food and water:
survival without food or water
Figure 5 - The survival curve of fruit flies in the absence of food and water
The number of live flies in high temperatures (37°C):
survival in high temperature
Figure 6 - The survival curve of fruit flies under high temperature
[T=37°C]
The seismo-tube experiment:
survival without food or water
Figure 7 - The modified fruit flies crawl higher in the seismo-tube experiment

Experiment 7: The reproductive curve of the modified fruit fly.

male-male and male-female mating
Figure 8 - The reproduction curve of the modified fruit fly
[n=5]

Experiment 8: Does the proportion of modified flies have an impact on mating rates of population?

male-male and male-female mating
Figure 9 - The impact that proportion of modified flies have on mating rates of population
[n=5]
Ignis Fly

Tongji_China iGEM 2017 Team
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