Team:Toronto/Wetlab

Wet Lab

Design, Parts & Hardware

What is LOV? Learn more about the rationale behind our project, and the parts that we designed and submitted (including but not limited to a light-regulated repressor, LacILOV, and an anti-CRISPR protein). Discover the hardware devices that we built for our assays, which are suitable for work with light-regulated proteins.

Experiments & Notebook

LOV letters: Detailed descriptions of the protocols and experiments that we carried out over the course of the project.

Interlab, Results, Demonstrate & Improve

I Will Always LOV You: Characterization of LacILOV, as well as our anti-CRISPR construct. Additionally, we outline the next steps towards a light-regulated switch for CRISPR using LacILOV.