Team:Wageningen UR/Part Collection

Collection of Parts

Here you can find our best collection of parts. We took five different fluorescent proteins (mVenus, sfGFP, mCerulean, mRFP and eYFP) and we split them to be used in Bimolecular Fluorescence Complementation (BiFC) analysis. All these parts have been characterized and our Best Part is a practical application of split proteins, used in the visualization system of Mantis.

BioBrick Code Part Name Designer
BBa_K2387045 mVenus controlled by inducible araC/pBAD promoter José Manuel
BBa_K2387046 Split mVenus controlled by inducible araC/pBAD promoter José Manuel
BBa_K2387047 sfGFP controlled by inducible araC/pBAD promoter José Manuel
BBa_K2387048 Split sfGFP controlled by inducible araC/pBAD promoter José Manuel
BBa_K2387052 mCerulean controlled by inducible araC/pBAD promoter José Manuel
BBa_K2387053 Split mCerulean controlled by inducible araC/pBAD promoter José Manuel
BBa_K2387054 mRFP controlled by inducible araC/pBAD promoter José Manuel
BBa_K2387055 Split mRFP controlled by inducible araC/pBAD promoter José Manuel
BBa_K2387003 eYFP controlled by inducible araC/pBAD promoter Bart
BBa_K2387065 eYFP[1-154]-nLeucine Zipper and eYFP[155-238]-cLeucine Zipper fusions controlled by inducible araC/pBAD promoter Bart
BBa_K2387032 CpxR-eYFPn and CpxR-eYPFc fusions controlled by inducible araC/pBAD promoter Bart