Team:WashU StLouis/Parts

Our Parts

Our team designed several parts that are intended to confer resistance to UV-B radiation in E. coli. Our basic parts encode radiation-resistance proteins, while our composite parts combine these proteins with an inducible Lac reporter and blue chromoprotein, a reporter.

Basic Parts

These basic parts are all coding sequences that came from foreign organisms, and we hoped would confer radiation resistance to E. Coli and our Cyanobacteria strain

Part Name Part Number Part Function
Dsup BBa_K2195000 Tardigrade DNA binding protein, provides radiation resistance
phrAc BBa_K2195001 Cyanobacteria DNA photolyase, provides radiation resistance
phrAt BBa_K2195006 Tardigrade DNA photolyase, provides radiation resistance

Composite Parts

Two of our composite parts contain Lac-inducible forms of our resistance genes. One is a reporter using the Blue Chromoprotein that we used as a control. The last is an antibiotic resistance gene that we improved by optimizing for E. Coli

Part Name Part Number Part Function
Dsup Full Gene BBa_K2195005 Synthesized gene of Lac Promoter, RBS and Dsup
uvsE Full Gene BBa_K2195008 Synthesized gene of Lac Promoter, RBS and uvsE
Blue Chromoprotein Reporter BBa_K2195007 Synthesized gene of Lac Promoter, RBS and BCP
Neomycin Phosphotransferase full gene BBa_K2195002 Confers antibiotic resistance to Kanamycin and Neomycin

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