The Washington iGEM wetlab team is in charge of the molecular biology side of the project. We developed a dCas9-sgRNA system designed to be inducible in yeast. Taking advantage of the violacein pathway as a proxy, we produced different colors to track metabolic activity. We also created and characterized Biobricks as well as Interlab.
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This season, Washington iGEM’s new wetlab team had a productive summer and fall. Having received an influx of new members we happily trained and worked hard alongside one another. For our main project, we were able to create the sgRNA expression system and successfully integrated it in yeast. We created and characterized two novel inducible promoter biobricks for yeast. Additionally, for the first time ever, we were able to participate in the iGEM 2017 Interlab collaboration alongside other iGEM teams across the globe.