Team:Worldshaper-Nanjing/Composite Part

INP-FBPA

BBa_K2513034

INP-FBPA

This part encodes a fusion protein that composed of INP and FBPA and designed to be expressed at the surface of E.coli or Synechococcus sp. PCC 7002. We replaced the 6His tag of INP-6His (BBa_K2509019) with a ferric iron binding protein FBPA (BBa_K2513032)to build this part.

BBa_K2509019 and BBa_K2513032

We directly replaced the 6His tag with FBPA without being deleted its stop codon to make a C-fusion protein. So that the whole part can only be transferred as a fusion protein with N part of INP and FBPA.

Results:

Constructions and expression results

Metal binding result 1

Metal binding result 2