Team:Worldshaper-Wuhan/Measurement
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Measurement
In our experiment, we use GFP as an indicative of the efficiency of miR-21-sponge-6s and miR-21-sponge-2s as mir-21 detectors, and quantified the mir-21 expression in the cells through the plasmid pHAGE-pre-mir-21. Then we gathered our data and plotted them into two standard curves on excel. Through comparing the slope of standard curves of miR-21-sponge-2s and miR-21-sponge-6s respectively, we determine that miR-12-sponge-2s is more sensitive than miR-21-sponge-6s.
