Team:XJTLU-CHINA/Protocols
Protocols
Basic Molecular Biology Techniques:
PCR using Q5 Hi-Fi DNA polymerase Master Mix
PCR purification using QIAquick PCR purification kit
Gel Extraction
Gibson assembly
Digestion (NEB enzymes)
Ligation
Transformation of plasmids in E.coli DH5ɑ
Isolation of plasmids in E.coli DH5ɑ
Preparation of competent Lactococcus lactis
Electroporation for Lactococcus lactis
Plasmid DNA isolation from Lactococcus lactis
Dot Blot
Anti-microbial peptides assay:
Pre-experiment: CFU measurement
Inhibition disc
AMPs minimal bactericidal concentration (MBC) assay
Biofilm formation test
Growth curve measurement
Nisin induction of gene expression in Lactococcus lactis:
Testing of S. aureus sensing pathway
Autolysis test
Collaborators and Supporters
