Parts

Welcome to the ZJU-China Parts Pages.This year, we designed 31 parts,and 18 of them had been submitted to the iGEM Registry follow the BioBricks assembly standard as described by RFC10.

We have a brief description of our parts here, but if you would like more detailed information on the parts, we strongly recommend you visit the corresponding parts pages on the iGEM BioBricks Registry.

Name	Type	Description	Designer	Length(bp)	Submitted
BBa_K2207000	Device	H3-RP27 expression system	Qianjin Jiang	2644
BBa_K2207001	Promoters	Hex promoter	Junbo Yang	3317
BBa_K2207002	Promoters	Ech42 promoter	Junbo Yang	3352
BBa_K2207003	Composite Parts	Trichoderma HR System I SOD-Tubulin dobule promoter	Yihe Zhang	3155	✔
BBa_K2207004	Composite Parts	Trichoderma HR System II Ech42-H3 double promoter	Yihe Zhang	4845	✔
BBa_K2207005	Composite Parts	Trichoderma HR System III L1-ADH1-RP27-L2	Yihe Zhang	903	✔
BBa_K2207006	Composite Parts	Trichoderma HR System IV L3-ADH1-RP27-L4	Yihe Zhang	903	✔
BBa_K2207007	Composite Parts	Trichoderma HR System V Homologous Binding SiteA	Yihe Zhang	1676	✔
BBa_K2207008	Composite Parts	Trichoderma HR System VI Homologous Binding SiteB	Yihe Zhang	1650	✔
BBa_K2207009	Coding Sequences	PhlF transcription factor	Qianjin Jiang	627	✔
BBa_K2207010	Other	Phl Operator	Yuxing Chen	825	✔
BBa_K2207011	Protein Generators	Phl Operator(BBa_K2207010) mRFP	Yuxing Chen	1545
BBa_K2207012	Composite Parts	DAPG sensor(PhlOF)	Qianjin Jiang	1450
BBa_K2207998	Composite Parts	2,4-DAPG PhlABCD Cluster	Junmin Qian	4332
BBa_K2207014	Composite Parts	PhlAC enzyme	Junmin Qian	5025
BBa_K2207015	Composite Parts	PhlBD enzyme	Junmin Qian	4212
BBa_K2207016	Composite Parts	PhlAB enzyme	Junmin Qian	5604
BBa_K2207017	Composite Parts	PhlCD enzyme	Junmin Qian	4646
BBa_K2207018	Devices	GPD-CYC1 expression system	Shisheng Li	382
BBa_K2207019	Translational units	TRPV1-Ferritin	Cheng Shen	4853
BBa_K2207020	Translational units	Ferritin	Cheng Shen	1173
BBa_K2207021	Promoters	Calcineurin-dependent response element (CDRE)	Shisheng Li	474	✔
BBa_K2207022	Protein Generators	CDRE(BBa_K2207021) oligoA-mrfp	Shisheng Li	1186	✔
BBa_K2207023	Coding Sequences	Mature serine protein from Paecilomyces lilacinus	Zifan Xie	882	✔
BBa_K2207024	Promoters	T7-mutant1	Yuxing Chen	42	✔
BBa_K2207025	Promoters	T7-mutant2	Yuxing Chen	42	✔
BBa_K2207026	Promoters	T7-mutant3	Yuxing Chen	42	✔
BBa_K2207027	Promoters	T7-mutant4	Yuxing Chen	42	✔
BBa_K2207028	Promoters	T7-mutant5	Yuxing Chen	42	✔
BBa_K2207029	Promoters	T7-mutant6	Yuxing Chen	42	✔
BBa_K2207030	Promoters	T7-mutant7	Yuxing Chen	42	✔
BBa_K2207031	Coding Sequences	PhlE efflux pump	Junming Qian	1272	✔
BBa_K2207997	Composite Parts	2,4-DAPG PhlABCD Cluster with T7 promoter and Double Terminator	Junming Qian	1272	✔
BBa_K2207036	Coding Sequencess	oligoA-mRFP	Shisheng Li	712	✔

Favorite Parts!

Our new Basic Parts in Trichoderma spp.

BBa_K2207003 ~ BBa_K2207008

We have collected and proved many new parts which can be used in Trichoderma spp. , including four promoters, two terminators and the selection marker hygB gene. And then we cloned them with homologous sequences on pSB1C3,for any researches who want to express genes in Trichoderma spp, they only need to integrate these parts with GOI and clone them into corresponding vectors(no matter what kinds of transduction methods or plasmids he used.) Click here to see how we constructed these systems.

Our calcium ion response promoter.

BBa_K2207021

We replaced the upstream activating sequence (UAS) of CYC1 promoter with 4 CDREs. This promoter responds to the cell calcium concentration increased, only designed for Saccharomyces cerevisiae and depended on S.cerevisiae's endogenous calcineurin and calmodulin. Click here to see how we use this part.

Best Composite Parts

BBa_K2207013

We got a new gene cluster through PCR from Pseudomonadaceae.fluorescens genome,phlABCD,coding four enzymes used to synthesis 2,4-DAPG.We have mutated some illegal enzyme sites and successfully detected the DAPG production.Click here to see our fantastic work.

Improve T7 promoters

BBa_K2207024 ~ BBa_K2207030