Achievements.html

Croc'n Cholera
iGEM UPS-INSA Toulouse 2017

Team

• Members
• Supports
• Attributions
• Collaborations

Strategy

• Description
• Design
• Parts

Model

• Overview
• Simulation
• Analysis
• Interface

Realisations

• Overview
• Notebook
• Clonings
• Results
• Contribution
• Protocols
• Safety

Human Practices

• Overview
• Silver
• Gold
• Public Engagement
• Microbioworld

Application

• Applied Design
• Testimonies
• Entrepreneurship
• Device Conception

Bronze medal

#1: We successfully registered for the competition, had an awesome summer and will proudly attended the Giant Jamboree!

#2: All the required competition deliverables were met in time for the due dates:

• We documented our project on the maximum pages possible on our wiki
• We thanked all the people that helped us in our Attribution page
• Our poster and prezi presentation were more than ready for the Giant Jamboree
• We proved that we made responsible experiments thanks to our Safety Form
• We successfully filled out 5 Judging Forms to compete for the corresponding awards
• We documented 6 BioBricks on our wiki and on the Registry
• We followed the DNA Submission Guidelines to submit our DNA samples

#3: We created an Attribution page entirely designed to thank all the people that helped throughout our adventure

#4: We successfully characterised the existing part BBa_J04450, an RFP coding device, in a new iGEM chassis: Vibrio harveyi. Characterisation was made thanks to Petri dish plating and microscopic fluorescence observations.

Silver medal

#1: Out of 17 Parts, we obtained 7 BioBricks by inserting them in pSB1C3. We successfully validated 2 of them: BBa_K2278001 and BBa_K2278021. Validations were respectively made thanks to bioluminescence essays and halos tests on Petri dishes.

#2: We are proud of all of the collaborations we made with teams around the world:

• For the Purdue team, we determined the Lactobacillus spp resistance at -20°C in a sucrose and milk mix.
• Thanks to the Vienna team, we obtained the part pGAP-RFP in P. pastoris.
• We participated to the Postcard Project organized by the Gröningen team and in exchange received postcard from all around the world.
• We participated to the European Meetup organized at TU Delft and to the French Meetup organized by the Pasteur Institute team. In each of these meetups, we obtained helpful feedbacks for our projects.
• We skyped with 5 teams around the world (Greece, Singapore, Purdue, Boston, Gröningen). Talking with teams from different backgrounds, tackling their observation on our project and trying to understand theirs was very enlightening.
• We filled out 17 surveys on various subjects.

#3: We carried out an ethical reflexion throughout all of our project, doing bibliographical research and meeting professionals and members of NGOs. Our major discussions and decisions are listed in our Human Practices Logbook.

Gold medal

#1: Our ethical reflexion went even further with the elaboration of 7 ethical matrices. Each matrix was a representation of our personal reflection on one key step of a water treatment product lifecycle. The outcomes of each matrix was carefully integrated in our project and could reshape part of it if needed.

#2: Modeling was a big part of our project, unavoidable considering the synthetic interactions of our three chassis. After building a standardized representation of all the genetic and molecular actors involved in communications, the challenge was to verify and simulate the functioning of our system through Ordinary Differential Equations. Then, sensitivity and robustness was analyzed thanks to Metabolic Control Analysis. Finally, a user-friendly interface was created for people non familiarized with ODEs.