LOCUS       Exported                2709 bp ds-DNA     circular SYN 14-OCT-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2017 from SnapGene 4.0.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      184..227
                     /label=bacterial terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    230..251
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    252..550
                     /label=pusp45
                     /note="/ApEinfo_fwdcolor=#faac61
                     /ApEinfo_revcolor=#faac61"
     misc_feature    519..524
                     /label=-35
                     /note="/ApEinfo_fwdcolor=#d6b295
                     /ApEinfo_revcolor=#d6b295"
     misc_feature    539..544
                     /label=-10
                     /note="/ApEinfo_fwdcolor=#ffef86
                     /ApEinfo_revcolor=#ffef86"
     misc_feature    646..681
                     /label=Repeat
                     /note="/ApEinfo_fwdcolor=#b1ff67
                     /ApEinfo_revcolor=#b1ff67"
     misc_feature    683..688
                     /label=BsaI Cutsite
                     /label=RE
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
     misc_feature    705..710
                     /label=BsaI Cutsite
                     /label=RE
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
     terminator      819..890
                     /label=rrnB T1 terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    891..911
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      912..969
                     /label=his operon terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    complement(1151..1765)
                     /label=rep (pMB1)
                     /note="/ApEinfo_fwdcolor=#f8d3a9
                     /ApEinfo_revcolor=#f8d3a9"
     terminator      1935..2029
                     /label=lambda t0 terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     CDS             complement(2050..2709)
                     /label=CmR
                     /note="/ApEinfo_fwdcolor=#b7e6d7
                     /ApEinfo_revcolor=#b7e6d7"
ORIGIN
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      241 cgcttctaga gccgggtata tttactaatc gctggacaag gctttttaca acaattatta
      301 ttgtgaccgc ttttgaagtt tttagtgcaa tcattatgac agcttttgga tttgcccaac
      361 ttcagtttat caaatttgtt gtttaccagt tagcgcctac acttttgctc aatattatct
      421 tagctgtagc cttacaattc cctttagaaa tcttttacag attaaagaaa agtcatgtaa
      481 gatacaatta gaaagtgttt tgtaatcata aagaaatatt aaggtggggt aggaatagta
      541 taatatgttt aataaatgca gtaatacagg ggcttttcaa gactgaagtc tagctgagac
      601 aaatagtgcg attacgaaat tttttagaca aaaatagtct acgaggtttt agagctatgc
      661 tgttttgaat ggtcccaaaa ctgagaccaa cattgccgat gataggtctc gacttgagaa
      721 agagggttaa taccagcagt cggatacctt cctattcttt ctgttaaagc gttttcatgt
      781 tataataggc aaaagaagag tagtgtgatg gaacaaacca aataaaacga aaggctcagt
      841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagtagc
      901 ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
      961 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
     1021 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1081 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1141 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
     1201 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1261 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1321 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1381 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1441 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1501 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1561 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1621 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1681 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1741 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1801 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1861 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1921 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     1981 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     2041 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
     2101 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
     2161 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
     2221 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     2281 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     2341 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     2401 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     2461 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     2521 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     2581 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     2641 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     2701 tttctccat
//
