MicroRNAs (miRNAs) constitute ideal building blocks for biochemical logic circuits as they inherently implement a NOR gate upon the gene they regulate. [7] In fact, any logic formula can be computed by miRNAs acting upon multiple transcriptional and post-transcriptional regulators and synthetic miRNAs encoded by them. [2] Therefore, we focused our efforts on developing a toolbox of highly modular and interchangeable parts, sufficient to assemble a plethora of customizable, multi-leveled circuits, enabling easy manipulations of miRNA inputs as well as circuit topologies to accommodate a less arduous application of classifying circuits by prospective iGEM teams and the entire SynBio community. We applied our engineered circuit in our cell line of interest, Caco-2, to actuate selective protein expression upon integration of multiple miRNA inputs, elicited from our model as quintessential to optimize our circuit’s classifying ability, demonstrating the feasibility of approaching colorectal cancer therapeutics with molecular-profiling-based expression systems of proteins of therapeutic potential.