LOCUS       piG17-1-007             3746 bp    DNA     circular UNA 25-JUL-2017
DEFINITION  PF-pLux-NcoI-protR max RBS-R CDS-HindIII-protE max RBS-protE
            CDS-SF.
ACCESSION   urn.local...b2-88wu342
VERSION     urn.local...b2-88wu342
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      7..101
                     /locus_tag="/note=""transcription terminator from phage
                     lambda"
                     /product
                     /label="lambda t0 terminator"
     CDS             233..1048
                     /locus_tag="/note=""confers resistance to kanamycin in
                     bacteria or G418 (Geneticin®) in eukaryotes"
                     /product="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /label="KanR"
     oriT            1204..1312
                     /locus_tag="/note=""incP origin of transfer"
                     /product
                     /label="oriT"
     rep_origin      1348..1936
                     /locus_tag="/note=""high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of rep lication"
                     /product
                     /label="ori"
     misc_feature    2122..2262
                     /locus_tag="/product="
                     /label="bom"
     CDS             complement(2364..2555)
                     /locus_tag="/product=""Rop protein, which maintains
                     plasmids at low copy number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
                     /label="rop"
     terminator      2735..2821
                     /locus_tag="/note=""transcription terminator T1 from the
                     E. coli rrnB ge ne"
                     /product
                     /label="rrnB T1 terminator"
     misc_feature    2782
                     /locus_tag="/product="
                     /label="A"
     misc_feature    2828..2849
                     /created_by="nicolashuber"
                     /label="PF"
     promoter        2850..2904
                     /created_by="nicolashuber"
                     /label="Plux"
     misc_feature    2905..2910
                     /created_by="nicolashuber"
                     /modified_by="nicolashuber"
                     /label="RS1 (NcoI)"
     misc_feature    2911..2934
                     /label="Protein R max RBS"
     CDS             2935..3410
                     /codon_start=1
                     /note="R, bacteriophage lambda lysozyme"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTGAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
                     /Color="#ff1a00"
                     /modified_by="gerdanie"
                     /label="R, bacteriophage lambda lysozyme CDS"
     misc_feature    3414..3419
                     /Recognition_pattern="A^AGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     RBS             3420..3446
                     /created_by="nicolashuber"
                     /label="Protein E max RBS"
     CDS             3447..>3719
                     /codon_start=1
                     /translation="MVRWTLWDTLAFLLLLSLLLPSLLIMFIPSTFKRPVSSWKALNL
                     RKTLLMASSVRLKPLNCSRLPCVYAQETLTFLLTQKKTCVKNYVRKE"
                     /label="proteinE phiX174"
     misc_feature    complement(3720..3722)
                     /label="rev compl stop codon"
     misc_feature    complement(3723..3725)
                     /label="rev compl stop codon"
     misc_feature    complement(3726..3746)
                     /label="rev SF"
ORIGIN      
        1 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
       61 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
      121 acgatttaaa tttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
      181 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
      241 tattcagcgt gaaacgagct gtagccgtcc gcgtctgaac agcaacatgg atgcggatct
      301 gtatggctat aaatgggcgc gtgataacgt gggtcagagc ggcgcgacca tttatcgtct
      361 gtatggcaaa ccggatgcgc cggaactgtt tctgaaacat ggcaaaggca gcgtggcgaa
      421 cgatgtgacc gatgaaatgg tgcgtctgaa ctggctgacc gaatttatgc cgctgccgac
      481 cattaaacat tttattcgca ccccggatga tgcgtggctg ctgaccaccg cgattccggg
      541 caaaaccgcg tttcaggtgc tggaagaata tccggatagc ggcgaaaaca ttgtggatgc
      601 gctggccgtg tttctgcgtc gtctgcatag cattccggtg tgcaactgcc cgtttaacag
      661 cgatcgtgtg tttcgtctgg cccaggcgca gagccgtatg aacaacggcc tggtggatgc
      721 gagcgatttt gatgatgaac gtaacggctg gccggtggaa caggtgtgga aagaaatgca
      781 taaactgctg ccgtttagcc cggatagcgt ggtgacccac ggcgatttta gcctggataa
      841 cctgattttc gatgaaggca aactgattgg ctgcattgat gtgggccgtg tgggcattgc
      901 ggatcgttat caggatctgg ccattctgtg gaactgcctg ggcgaattta gcccgagcct
      961 gcaaaaacgt ctgtttcaga aatatggcat tgataatccg gatatgaaca aactgcaatt
     1021 tcatctgatg ctggatgaat ttttctaata attaattgga caagggtcct tttccgctgc
     1081 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     1141 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     1201 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     1261 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg
     1321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1741 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1981 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2041 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2101 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2161 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2221 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     2281 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2341 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     2401 cagctgcggt aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca
     2461 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     2521 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     2581 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     2641 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     2701 cggcgggggc gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca
     2761 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
     2821 gatgcctgaa ttcgcggccg cttctagaga cctgtaggat cgtacaggtt tacgcaagaa
     2881 aatggtttgt tatagtcgaa taaaccatgg tttaacaagt attaaagggt caaaatgtag
     2941 aaatcaataa tcaacgtaag gcgttcctcg atatgctggc gtggtcggag ggaactgata
     3001 acggacgtca gaaaaccaga aatcatggtt atgacgtcat tgtaggcgga gagctattta
     3061 ctgattactc cgatcaccct cgcaaacttg tcacgctaaa cccaaaactc aaatcaacag
     3121 gcgccggacg ctaccagctt ctttcccgtt ggtgggatgc ctaccgcaag cagcttggcc
     3181 tgaaagactt ctctccgaaa agtcaggacg ctgtggcatt gcagcagatt aaggagcgtg
     3241 gcgctttacc tatgattgat cgtggtgata tccgtcaggc aatcgaccgt tgcagcaata
     3301 tctgggcttc actgccgggc gctggttatg gtcagttcga gcataaggct gacagcctga
     3361 ttgcaaaatt caaagaagcg ggcggaacgg tcagagagat tgatgtataa taaaagcttc
     3421 acaataagaa tttaaggagg taaaatatgg tacgctggac tttgtgggat accctcgctt
     3481 tcctgctcct gttgagttta ttgctgccgt cattgcttat tatgttcatc ccgtcaacat
     3541 tcaaacggcc tgtctcatca tggaaggcgc tgaatttacg gaaaacatta ttaatggcgt
     3601 cgagcgtccg gttaaagccg ctgaattgtt cgcgtttacc ttgcgtgtac gcgcaggaaa
     3661 cactgacgtt cttactgacg cagaagaaaa cgtgcgtcaa aaattacgtg cggaaggagt
     3721 aataatacta gtagcggccg ctgcag
//
