LOCUS       piG17-1-002             4207 bp    DNA     circular UNA 01-NOV-2017
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...k0-88wxito
VERSION     urn.local...k0-88wxito
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     source          <1..2827
                     /organism
                     /label="source"
     terminator      7..101
                     /locus_tag="/note=""transcription terminator from phage
                     lambda"
                     /product
                     /label="lambda t0 terminator"
     CDS             233..1048
                     /locus_tag="/note=""confers resistance to kanamycin in
                     bacteria or G418 (Geneticin®) in eukaryotes"
                     /product="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /label="KanR"
     oriT            1204..1312
                     /locus_tag="/note=""incP origin of transfer"
                     /product
                     /label="oriT"
     rep_origin      1348..1936
                     /locus_tag="/note=""high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of rep lication"
                     /product
                     /label="ori"
     misc_feature    2122..2262
                     /locus_tag="/product="
                     /label="bom"
     CDS             complement(2364..2555)
                     /locus_tag="/product=""Rop protein, which maintains
                     plasmids at low copy number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
                     /label="rop"
     terminator      2735..2821
                     /locus_tag="/note=""transcription terminator T1 from the
                     E. coli rrnB ge ne"
                     /product
                     /label="rrnB T1 terminator"
     DNA_probe_bind  2788..2867
                     /annotation_group="a58c9d74-117a-4836-8cdb-6048a60f9c0b"
                     /Mismatches=0
                     /label="oiG17-00_LCR_bridge_backbone_PF_Plux"
     misc_feature    2827..2849
                     /created_by="cherasv"
                     /label="BioBrick Prefix"
     promoter        2850..2910
                     /created_by="cherasv"
                     /label="Plux"
     DNA_probe_bind  2871..2950
                     /annotation_group="fb0b6550-98c5-4810-bcb8-6f3632b42696"
                     /Mismatches=0
                     /label="oiG17-00_LCR_bridge_Plux gfp"
     RBS             2911..2945
                     /locus_tag="/product="
                     /label="strong RBS"
     source          <2911..>3671
                     /organism
                     /label="source"
     CDS             2946..3671
                     /locus_tag="/note=""sfGFP"
                     /product="/translation=""MSKGEELFTGVVPILVELDGDVNGHKFSVRGEG
                     EGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKD
                     DGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNG
                     IKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYKRP"
                     /label="sfGFP"
     DNA_probe_bind  3638..3717
                     /annotation_group="941183dc-ff48-4302-be9e-485921d09d14"
                     /Mismatches=0
                     /label="oiG17-00_LCR_bridge_gfp_bfr"
     misc_feature    3672..3677
                     /Recognition_pattern="GAGCT^C"
                     /label="SacI"
                     /note="Geneious type: restriction site"
     misc_feature    3678..3703
                     /synthetic_RBS,_Salis_calculator_TIR=52140
                     /created_by="cherasv"
                     /label="RBS"
     CDS             3704..4180
                     /created_by="David"
                     /label="hdBFR"
     Optimized_Codon 3713..3715
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3716..3718
                     /Relative_Adaptiveness="1.0"
                     /Original="ACT"
                     /label="ACC"
     Optimized_Codon 3725..3727
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATC"
     Optimized_Codon 3728..3730
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     Optimized_Codon 3731..3733
                     /Relative_Adaptiveness="1.0"
                     /Original="TAT"
                     /label="TAC"
     Optimized_Codon 3734..3736
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 3746..3748
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 3749..3751
                     /Relative_Adaptiveness="1.0"
                     /Original="GGA"
                     /label="GGT"
     Optimized_Codon 3752..3754
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     Optimized_Codon 3755..3757
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3758..3760
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3761..3763
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTT"
     Optimized_Codon 3764..3766
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCG"
     misc_feature    3766..3769
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3770..3772
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     misc_feature    3774..3779
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI"
                     /note="Geneious type: restriction site"
     misc_feature    3775..3778
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3779..3781
                     /Relative_Adaptiveness="1.0"
                     /Original="TTT"
                     /label="TTC"
     Optimized_Codon 3782..3784
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3785..3787
                     /Relative_Adaptiveness="1.0"
                     /Original="CAT"
                     /label="CAC"
     misc_feature    3788..3791
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3788..3790
                     /Relative_Adaptiveness="1.0"
                     /Original="GCC"
                     /label="GCG"
     Optimized_Codon 3791..3793
                     /Relative_Adaptiveness="1.0"
                     /Original="CGG"
                     /label="CGT"
     Optimized_Codon 3797..3799
                     /Relative_Adaptiveness="1.0"
                     /Original="TTT"
                     /label="TTC"
     Optimized_Codon 3812..3814
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 3821..3823
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 3824..3826
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     Optimized_Codon 3827..3829
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3830..3832
                     /Relative_Adaptiveness="1.0"
                     /Original="GTG"
                     /label="GTT"
     Optimized_Codon 3833..3835
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3836..3838
                     /Relative_Adaptiveness="1.0"
                     /Original="TAT"
                     /label="TAC"
     Optimized_Codon 3839..3841
                     /Relative_Adaptiveness="1.0"
                     /Original="CAT"
                     /label="CAC"
     misc_feature    3842..3846
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3845..3847
                     /Relative_Adaptiveness="1.0"
                     /Original="TCC"
                     /label="TCT"
     Optimized_Codon 3848..3850
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     misc_feature    3849..3852
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3851..3853
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3854..3856
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3866..3868
                     /Relative_Adaptiveness="1.0"
                     /Original="GCC"
                     /label="GCG"
     misc_feature    3868..3872
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3869..3871
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3875..3877
                     /Relative_Adaptiveness="1.0"
                     /Original="TAT"
                     /label="TAC"
     Optimized_Codon 3878..3880
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     misc_feature    3879..3882
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3881..3883
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3884..3886
                     /Relative_Adaptiveness="1.0"
                     /Original="CGC"
                     /label="CGT"
     Optimized_Codon 3887..3889
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 3890..3892
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3893..3895
                     /Relative_Adaptiveness="1.0"
                     /Original="TTT"
                     /label="TTC"
     misc_feature    3895..3899
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    3895..3899
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3905..3907
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3908..3910
                     /Relative_Adaptiveness="1.0"
                     /Original="CCA"
                     /label="CCG"
     Optimized_Codon 3914..3916
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 3917..3919
                     /Relative_Adaptiveness="0.21586"
                     /Original="CAG"
                     /label="CAA"
     misc_feature    3922..3926
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    3922..3926
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3926..3928
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3938..3940
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 3947..3949
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3953..3955
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3971..3973
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     misc_feature    3973..3977
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    3973..3977
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    3976..3981
                     /Recognition_pattern="RGCGC^Y"
                     /annotation_group="RGCGCY"
                     /label="HaeII"
                     /note="Geneious type: restriction site"
     misc_feature    3977..3980
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3977..3979
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCG"
     Optimized_Codon 3980..3982
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3983..3985
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3989..3991
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3992..3994
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3998..4000
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 4001..4003
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     Optimized_Codon 4004..4006
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 4010..4012
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 4013..4015
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCG"
     misc_feature    4014..4019
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI"
                     /note="Geneious type: restriction site"
     misc_feature    4015..4018
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 4016..4018
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 4022..4024
                     /Relative_Adaptiveness="1.0"
                     /Original="TAT"
                     /label="TAC"
     Optimized_Codon 4025..4027
                     /Relative_Adaptiveness="1.0"
                     /Original="GCC"
                     /label="GCG"
     misc_feature    4028..4032
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 4028..4030
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 4031..4033
                     /Relative_Adaptiveness="1.0"
                     /Original="AGC"
                     /label="TCT"
     Optimized_Codon 4037..4039
                     /Relative_Adaptiveness="1.0"
                     /Original="CAT"
                     /label="CAC"
     Optimized_Codon 4040..4042
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 4046..4048
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTT"
     Optimized_Codon 4049..4051
                     /Relative_Adaptiveness="1.0"
                     /Original="AGC"
                     /label="TCT"
     Optimized_Codon 4052..4054
                     /Relative_Adaptiveness="1.0"
                     /Original="CGC"
                     /label="CGT"
     Optimized_Codon 4055..4057
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     misc_feature    4063..4066
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 4064..4066
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATC"
     misc_feature    4065..4068
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 4070..4072
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 4073..4075
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 4076..4078
                     /Relative_Adaptiveness="1.0"
                     /Original="CGC"
                     /label="CGT"
     Optimized_Codon 4079..4081
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 4088..4090
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 4091..4093
                     /Relative_Adaptiveness="1.0"
                     /Original="CAT"
                     /label="CAC"
     misc_feature    4095..4098
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 4109..4111
                     /Relative_Adaptiveness="1.0"
                     /Original="ACG"
                     /label="ACC"
     Optimized_Codon 4115..4117
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     misc_feature    4117..4121
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     Optimized_Codon 4118..4120
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     misc_feature    4123..4126
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 4124..4126
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 4130..4132
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 4136..4138
                     /Relative_Adaptiveness="1.0"
                     /Original="GGA"
                     /label="GGT"
     Optimized_Codon 4145..4147
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     Optimized_Codon 4148..4150
                     /Relative_Adaptiveness="1.0"
                     /Original="TAT"
                     /label="TAC"
     misc_feature    4157..4160
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 4157..4159
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCG"
     misc_feature    4162..4165
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 4163..4165
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 4166..4168
                     /Relative_Adaptiveness="1.0"
                     /Original="CGC"
                     /label="CGT"
     DNA_probe_bind  4168..40
                     /annotation_group="52759a50-7c06-4387-86ca-7d3fe45e02c4"
                     /Mismatches=0
                     /label="oiDG17-00_LCR_bridge_bfr_SF_backbone"
     misc_feature    4177..4180
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    4181..>4207
                     /created_by="cherasv"
                     /modified_by="cherasv"
                     /label="Suffix+TATTTA"
     misc_feature    4182..4187
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI"
                     /note="Geneious type: restriction site"
     misc_feature    4189..4196
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     misc_feature    4191..4194
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    4196..4201
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
       61 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
      121 acgatttaaa tttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
      181 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
      241 tattcagcgt gaaacgagct gtagccgtcc gcgtctgaac agcaacatgg atgcggatct
      301 gtatggctat aaatgggcgc gtgataacgt gggtcagagc ggcgcgacca tttatcgtct
      361 gtatggcaaa ccggatgcgc cggaactgtt tctgaaacat ggcaaaggca gcgtggcgaa
      421 cgatgtgacc gatgaaatgg tgcgtctgaa ctggctgacc gaatttatgc cgctgccgac
      481 cattaaacat tttattcgca ccccggatga tgcgtggctg ctgaccaccg cgattccggg
      541 caaaaccgcg tttcaggtgc tggaagaata tccggatagc ggcgaaaaca ttgtggatgc
      601 gctggccgtg tttctgcgtc gtctgcatag cattccggtg tgcaactgcc cgtttaacag
      661 cgatcgtgtg tttcgtctgg cccaggcgca gagccgtatg aacaacggcc tggtggatgc
      721 gagcgatttt gatgatgaac gtaacggctg gccggtggaa caggtgtgga aagaaatgca
      781 taaactgctg ccgtttagcc cggatagcgt ggtgacccac ggcgatttta gcctggataa
      841 cctgattttc gatgaaggca aactgattgg ctgcattgat gtgggccgtg tgggcattgc
      901 ggatcgttat caggatctgg ccattctgtg gaactgcctg ggcgaattta gcccgagcct
      961 gcaaaaacgt ctgtttcaga aatatggcat tgataatccg gatatgaaca aactgcaatt
     1021 tcatctgatg ctggatgaat ttttctaata attaattgga caagggtcct tttccgctgc
     1081 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     1141 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     1201 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     1261 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg
     1321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1741 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1981 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2041 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2101 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2161 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2221 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     2281 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2341 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     2401 cagctgcggt aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca
     2461 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     2521 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     2581 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     2641 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     2701 cggcgggggc gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca
     2761 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
     2821 gatgcctgaa ttcgcggccg cttctagaga cctgtaggat cgtacaggtt tacgcaagaa
     2881 aatggtttgt tatagtcgaa taaaccatgg gataaatgct tcaataatat gaggaggtga
     2941 tctagatgag caaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     3001 atggtgatgt taatgggcac aaattttctg tccgtggaga gggtgaaggt gatgctacaa
     3061 acggaaaact cacccttaaa tttatttgca ctactggaaa actacctgtt ccgtggccaa
     3121 cacttgtcac tactctgacc tatggtgttc aatgcttttc ccgttatccg gatcacatga
     3181 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa cgcactatat
     3241 ctttcaaaga tgacgggacc tacaagacgc gtgctgaagt caagtttgaa ggtgataccc
     3301 ttgttaatcg tatcgagtta aagggtattg attttaaaga agatggaaac attcttggac
     3361 acaaactcga gtacaacttt aactcacaca atgtatacat cacggcagac aaacaaaaga
     3421 atggaatcaa agctaacttc aaaattcgcc acaacgttga agatggttcc gttcaactag
     3481 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
     3541 attacctgtc gacacaatct gtcctttcga aagatcccaa cgaaaagcgt gaccacatgg
     3601 tccttcttga gtttgtaact gctgctggga ttacacatgg catggatgag ctctacaaaa
     3661 ggccttaata agagctcaag gaaaagactt ctaggaggtt ccaatgaaag gtgacaccaa
     3721 agttatcaac tacctgaaca aactgctggg taacgaactg gttgcgatca accagtactt
     3781 cctgcacgcg cgtatgttca aaaactgggg tctgaaacgt ctgaacgacg ttgaatacca
     3841 cgaatctatc gacgaacaca aacacgcgga ccgttacatc gaacgtatcc tgttcctgga
     3901 aggtctgccg aacctgcaag acctgggtaa actgaacatc ggtgaagacg ttgaagaaat
     3961 gctgcgttct gacctggcgc tggaactgga cggtgcgaaa aacctgcgtg aagcgatcgg
     4021 ttacgcggac tctgttcacg actacgtttc tcgtgacatg atgatcgaaa tcctgcgtga
     4081 cgaagaaggt cacatcgact ggctggaaac cgaactggac ctgatccaga aaatgggtct
     4141 gcaaaactac ctgcaagcgc agatccgtga agaaggttaa tactagtagc ggccgctgca
     4201 gtattta
//
