LOCUS       piG17-1-004_Plux        3837 bp    DNA     circular UNA 01-NOV-2017
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...6q-88wu31v
VERSION     urn.local...6q-88wu31v
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     source          <1..2827
                     /organism
                     /label="source"
     terminator      7..101
                     /locus_tag="/note=""transcription terminator from phage
                     lambda"
                     /product
                     /label="lambda t0 terminator"
     CDS             233..1048
                     /locus_tag="/note=""confers resistance to kanamycin in
                     bacteria or G418 (Geneticin®) in eukaryotes"
                     /product="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /label="KanR"
     oriT            1204..1312
                     /locus_tag="/note=""incP origin of transfer"
                     /product
                     /label="oriT"
     rep_origin      1348..1936
                     /locus_tag="/note=""high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of rep lication"
                     /product
                     /label="ori"
     misc_feature    2122..2262
                     /locus_tag="/product="
                     /label="bom"
     CDS             complement(2364..2555)
                     /locus_tag="/product=""Rop protein, which maintains
                     plasmids at low copy number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
                     /label="rop"
     terminator      2735..2821
                     /locus_tag="/note=""transcription terminator T1 from the
                     E. coli rrnB ge ne"
                     /product
                     /label="rrnB T1 terminator"
     DNA_probe_bind  2788..2867
                     /created_by="cherasv"
                     /Sequence="CCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGAATTCG
                     CGGCCGCTTCTAGAGACCTGTAGGATCGTACAG"
                     /Mismatches=0
                     /annotation_group="f4394ad1-03ca-464b-8beb-5c94a9e410ee"
                     /label="oiG17-00_LCR_bridge_Backbone_Plux"
     misc_feature    2828..2849
                     /created_by="cherasv"
                     /modified_by="cherasv"
                     /label="BioBrick Prefix"
     promoter        2850..2910
                     /created_by="cherasv"
                     /label="Plux "
     DNA_probe_bind  2871..2950
                     /annotation_group="76d5ed23-e71c-47b8-a61d-aa0faf243d01"
                     /Mismatches=0
                     /label="oiG-00_LCR_bridge_Plux_p28"
     misc_feature    2911..2947
                     /Salis_TIR=32320
                     /created_by="cherasv"
                     /modified_by="cherasv"
                     /label="gcggt+RBS"
     source          <2911..>3049
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="CIT135"
                     /db_xref="taxon:287"
                     /label="source Pseudomonas aeruginosa"
     source          <2911..>2947
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="CIT135"
                     /db_xref="taxon:287"
                     /label="source Pseudomonas aeruginosa"
     CDS             <2948..>3040
                     /codon_start=1
                     /gene="azu"
                     /note="precursor"
                     /transl_table=11
                     /product="azurin"
                     /protein_id="AAA25730.1"
                     /translation="MLRKLAAVSLLSLLSAPLLAAECSVDIQGNDQMQFNTNAITVDK
                     SCKQFTVNLSHPGNLPKNVMGHNWVLSTAADMQGVVTDGMASGLDKDYLKPDDSRVIA
                     HTKLIGSGEKDSVTFDVSKLKEGEQYMFFCTFPGHSALMKGTLTLK"
                     /modified_by="cherasv"
                     /label="azup28 CDS"
     source          <2948..>3040
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="CIT135"
                     /db_xref="taxon:287"
                     /label="source Pseudomonas aeruginosa"
     Optimized_Codon 2951..2953
                     /Relative_Adaptiveness="1.0"
                     /Original="GTA"
                     /label="GTT"
     Optimized_Codon 2957..2959
                     /Relative_Adaptiveness="1.0"
                     /Original="AGC"
                     /label="TCT"
     Optimized_Codon 2963..2965
                     /Relative_Adaptiveness="1.0"
                     /Original="GCC"
                     /label="GCG"
     Optimized_Codon 2966..2968
                     /Relative_Adaptiveness="1.0"
                     /Original="GCC"
                     /label="GCG"
     Optimized_Codon 2978..2980
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 2981..2983
                     /Relative_Adaptiveness="1.0"
                     /Original="GTG"
                     /label="GTT"
     Optimized_Codon 2984..2986
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTT"
     Optimized_Codon 2993..2995
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 2999..3001
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCG"
     Optimized_Codon 3002..3004
                     /Relative_Adaptiveness="1.0"
                     /Original="TCC"
                     /label="TCT"
     Optimized_Codon 3005..3007
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     DNA_probe_bind  3010..3089
                     /annotation_group="97b23864-f838-4de3-ac40-bf55b3dae594"
                     /Mismatches=0
                     /label="oiG-00_LCR_bridge_p28_gfp"
     Optimized_Codon 3014..3016
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3017..3019
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3026..3028
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3029..3031
                     /Relative_Adaptiveness="1.0"
                     /Original="CCC"
                     /label="CCG"
     misc_feature    3041..3046
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI, SstI"
                     /note="Geneious type: restriction site"
     misc_feature    <3041..3049
                     /created_by="cherasv"
                     /modified_by="cherasv"
                     /label="SacI_GTC_ext"
     source          <3041..>3049
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="CIT135"
                     /db_xref="taxon:287"
                     /label="source Pseudomonas aeruginosa"
     misc_feature    3042..3045
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    3050..3055
                     /Recognition_pattern="G^TCGAC"
                     /label="SalI"
                     /note="Geneious type: restriction site"
     RBS             3056..3090
                     /locus_tag="/product="
                     /label="strong RBS"
     source          <3056..>3816
                     /organism
                     /label="source"
     CDS             3091..3816
                     /locus_tag="/note=""sfGFP"
                     /product="/translation=""MSKGEELFTGVVPILVELDGDVNGHKFSVRGEG
                     EGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKD
                     DGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNG
                     IKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYKRP"
                     /label="sfGFP"
     DNA_probe_bind  3798..40
                     /created_by="cherasv"
                     /Sequence="CTACAAAAGGCCTTAATAATACTAGTAGCGGCCGCTGCAGGTCTTGG
                     ACTCCTGTTGATAGATCCAGTAATGACCTCAGA"
                     /Mismatches=0
                     /annotation_group="fc1fbc11-6f80-4502-89f1-06810df5d4f9"
                     /label="oiG17-00_LCR_bridge_gfp_SF_backbone"
     misc_feature    3817..3837
                     /created_by="cherasv"
                     /label="BioBrickSuffix"
ORIGIN      
        1 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
       61 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
      121 acgatttaaa tttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
      181 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
      241 tattcagcgt gaaacgagct gtagccgtcc gcgtctgaac agcaacatgg atgcggatct
      301 gtatggctat aaatgggcgc gtgataacgt gggtcagagc ggcgcgacca tttatcgtct
      361 gtatggcaaa ccggatgcgc cggaactgtt tctgaaacat ggcaaaggca gcgtggcgaa
      421 cgatgtgacc gatgaaatgg tgcgtctgaa ctggctgacc gaatttatgc cgctgccgac
      481 cattaaacat tttattcgca ccccggatga tgcgtggctg ctgaccaccg cgattccggg
      541 caaaaccgcg tttcaggtgc tggaagaata tccggatagc ggcgaaaaca ttgtggatgc
      601 gctggccgtg tttctgcgtc gtctgcatag cattccggtg tgcaactgcc cgtttaacag
      661 cgatcgtgtg tttcgtctgg cccaggcgca gagccgtatg aacaacggcc tggtggatgc
      721 gagcgatttt gatgatgaac gtaacggctg gccggtggaa caggtgtgga aagaaatgca
      781 taaactgctg ccgtttagcc cggatagcgt ggtgacccac ggcgatttta gcctggataa
      841 cctgattttc gatgaaggca aactgattgg ctgcattgat gtgggccgtg tgggcattgc
      901 ggatcgttat caggatctgg ccattctgtg gaactgcctg ggcgaattta gcccgagcct
      961 gcaaaaacgt ctgtttcaga aatatggcat tgataatccg gatatgaaca aactgcaatt
     1021 tcatctgatg ctggatgaat ttttctaata attaattgga caagggtcct tttccgctgc
     1081 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     1141 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     1201 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     1261 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg
     1321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1741 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1981 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2041 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2101 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2161 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2221 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     2281 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2341 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     2401 cagctgcggt aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca
     2461 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     2521 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     2581 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     2641 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     2701 cggcgggggc gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca
     2761 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
     2821 gatgcctgaa ttcgcggccg cttctagaga cctgtaggat cgtacaggtt tacgcaagaa
     2881 aatggtttgt tatagtcgaa taaaccatgg ccttatatca tcatcaattc actaaggggg
     2941 ttacattatg gttctgtcta ccgcggcgga catgcagggt gttgttaccg acggtatggc
     3001 gtctggtctg gacaaagact acctgaaacc ggactaataa gagctcgtcg tcgacgataa
     3061 atgcttcaat aatatgagga ggtgatctag atgagcaaag gagaagaact tttcactgga
     3121 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtccgt
     3181 ggagagggtg aaggtgatgc tacaaacgga aaactcaccc ttaaatttat ttgcactact
     3241 ggaaaactac ctgttccgtg gccaacactt gtcactactc tgacctatgg tgttcaatgc
     3301 ttttcccgtt atccggatca catgaaacgg catgactttt tcaagagtgc catgcccgaa
     3361 ggttatgtac aggaacgcac tatatctttc aaagatgacg ggacctacaa gacgcgtgct
     3421 gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg tattgatttt
     3481 aaagaagatg gaaacattct tggacacaaa ctcgagtaca actttaactc acacaatgta
     3541 tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat tcgccacaac
     3601 gttgaagatg gttccgttca actagcagac cattatcaac aaaatactcc aattggcgat
     3661 ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgtcct ttcgaaagat
     3721 cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc tgggattaca
     3781 catggcatgg atgagctcta caaaaggcct taataatact agtagcggcc gctgcag
//
