LOCUS       piG17-2-001             2906 bp    DNA     circular UNA 22-SEP-2017
DEFINITION  pSEVA361.
ACCESSION   urn.local...ok-7v6znw4
VERSION     urn.local...ok-7v6znw4
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
FEATURES             Location/Qualifiers
     source          <1..>2030
                     /organism
     primer_bind     1..25
                     /standard_name="oiG17-043"
     misc_feature    complement(1..25)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-51"
     terminator      7..101
                     /locus_tag="/product="
                     /standard_name="lambda t0 terminator|terminator"
     primer_bind     7..28
                     /standard_name="oDG257"
     primer_bind     7..28
                     /standard_name="oDG183"
     misc_feature    complement(24..42)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="QE-rev"
     misc_feature    complement(48..72)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oDG253"
     misc_feature    complement(52..71)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oDG210"
     misc_feature    complement(81..101)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oDG264"
     misc_feature    complement(89..108)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oDG433"
     misc_feature    complement(108..127)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oDG128"
     CDS             252..911
                     /locus_tag="/product=""
                     /translation=""MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITA
                     F
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
                     /standard_name="chloramphenicol_acetyltransferase(CAT)_ORF
                     Selectab le marker|CDS"
     primer_bind     484..500
                     /standard_name="oDG302"
     misc_feature    complement(568..584)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oDG303"
     primer_bind     877..896
                     /standard_name="oDG175"
     misc_feature    925..1170
                     /locus_tag="/product="
                     /standard_name="oriT|misc_feature"
     primer_bind     935..1004
                     /standard_name="oiG17-013"
     primer_bind     935..1004
                     /standard_name="oDG417"
     misc_feature    complement(947..964)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-012"
     misc_feature    complement(947..964)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oDG414"
     primer_bind     965..986
                     /standard_name="oiG17-011"
     primer_bind     965..986
                     /standard_name="oDG413"
     misc_feature    complement(1062..1081)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oDG176"
     misc_feature    complement(1148..1172)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oDG301"
     misc_feature    complement(1167..1194)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oDG042"
     primer_bind     1174..1214
                     /standard_name="oDG043"
     rep_origin      1215..1760
     unsure          complement(1767..1770)
                     /locus_tag="/note=""CAAAA > CAAA in sequencing 14.11.2014"
     misc_feature    complement(1891..1924)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oDG044"
     primer_bind     1905..1922
                     /standard_name="oDG045"
     primer_bind     1936..1955
                     /standard_name="oDG127"
     terminator      complement(1938..2024)
                     /locus_tag="/note=""transcription terminator T1 from the
                     E. coli rrnB ge ne"
                     /standard_name="rrnB T1 terminator"
     primer_bind     1938..1958
                     /standard_name="oDG263"
     misc_difference 1990
                     /standard_name="A"
     primer_bind     2007..2030
                     /standard_name="oiG17-50"
     misc_feature    complement(2007..2030)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-008"
     misc_feature    complement(2009..2030)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oDG262"
     misc_feature    2031..2906
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     primer_bind     2053..2070
                     /standard_name="oDG388"
     misc_feature    complement(2066..2084)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-60"
     RBS             2088..2123
                     /standard_name="100k RBS"
     CDS             2124..2879
                     /codon_start=1
                     /product="LuxR-Barcode"
                     /note="luxR (C0062)"
                     /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLA
                     IIYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAV
                     NKKSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPL
                     IVPSLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHL
                     TNAQMKLNTTNRCQSISKAILTGAIDCPYFKN*"
                     /standard_name="LuxR-Barcode CDS"
     source          <2124..>2879
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     2125..2144
                     /standard_name="oiG17-59"
     misc_feature    complement(2888..2906)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-53"
ORIGIN      
        1 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
       61 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
      121 acgatttaaa ttggcgaaaa tgagacgttg atcggcacgt aagaggttcc aactttcacc
      181 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
      241 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
      301 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      361 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      421 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa
      481 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
      541 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
      601 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
      661 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
      721 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
      781 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
      841 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
      901 gcggggcgta atttgacttt tgtccttttc cgctgcataa ccctgcttcg gggtcattat
      961 agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt gccaaagggt
     1021 tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg
     1081 cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa
     1141 cgggaatcct gctctgcgag gctggccgta ggccggccct agaaatattt tatctgatta
     1201 ataagatgat cttcttgaga tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa
     1261 aaaaccgcct tgcagggcgg tttttcgaag gttctctgag ctaccaactc tttgaaccga
     1321 ggtaactggc ttggaggagc gcagtcacca aaacttgtcc tttcagttta gccttaaccg
     1381 gcgcatgact tcaagactaa ctcctctaaa tcaattacca gtggctgctg ccagtggtgc
     1441 ttttgcatgt ctttccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1501 ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct acccggaact
     1561 gagtgtcagg cgtggaatga gacaaacgcg gccataacag cggaatgaca ccggtaaacc
     1621 gaaaggcagg aacaggagag cgcacgaggg agccgccagg gggaaacgcc tggtatcttt
     1681 atagtcctgt cgggtttcgc caccactgat ttgagcgtca gatttcgtga tgcttgtcag
     1741 gggggcggag cctatggaaa aacggctttg ccgcggccct ctcacttccc tgttaagtat
     1801 cttcctggca tcttccagga aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag
     1861 tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaatatatcc ggcgcgccca
     1921 gctgtctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     1981 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct gaattcgcgg
     2041 ccgcttctag agttgacggc tagctcagtc ctaggtacag tgctagcagg aataaacacg
     2101 cagctaacag aggagggaac aacatgaaaa acataaatgc cgacgacaca tacagaataa
     2161 ttaataaaat taaagcttgt agaagcaata atgatattaa tcaatgctta tctgatatga
     2221 ctaaaatggt acattgtgaa tattatttac tcgcgatcat ttatcctcat tctatggtta
     2281 aatctgatat ttcaatccta gataattacc ctaaaaaatg gaggcaatat tatgatgacg
     2341 ctaatttaat aaaatatgat cctatagtag attattctaa ctccaatcat tcaccaatta
     2401 attggaatat atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta attaaagaag
     2461 cgaaaacatc aggtcttatc actgggttta gtttccctat tcatacggct aacaatggct
     2521 tcggaatgct tagttttgca cattcagaaa aagacaacta tatagatagt ttatttttac
     2581 atgcgtgtat gaacatacca ttaattgttc cttctctagt tgataattat cgaaaaataa
     2641 atatagcaaa taataaatca aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt
     2701 gggcatgcga aggaaaaagc tcttgggata tttcaaaaat attaggttgc agtgagcgta
     2761 ctgtcacttt ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa
     2821 gtatttctaa agcaatttta acaggagcaa ttgattgccc atactttaaa aattaataag
     2881 tcgactacta gtagcggccg ctgcag
//
