LOCUS       piG17-1-001             5065 bp    DNA     circular UNA 09-OCT-2017
DEFINITION  pSEVA291.
ACCESSION   urn.local...jx-7v6xw87
VERSION     urn.local...jx-7v6xw87
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
FEATURES             Location/Qualifiers
     source          <1..2827
                     /organism
     primer_bind     1..25
                     /standard_name="oiG17-043"
     misc_feature    complement(1..25)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-51"
     terminator      7..101
                     /locus_tag="/note=""transcription terminator from phage
                     lambda"
                     /standard_name="lambda t0 terminator"
     CDS             233..1048
                     /locus_tag="/note=""confers resistance to kanamycin in
                     bacteria or G418 (Geneticin®) in eukaryotes"
                     /product="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /standard_name="KanR"
     primer_bind     1079..1148
                     /standard_name="oiG17-013"
     misc_feature    complement(1091..1108)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-012"
     primer_bind     1109..1130
                     /standard_name="oiG17-011"
     oriT            1204..1312
                     /locus_tag="/note=""incP origin of transfer"
                     /standard_name="oriT"
     rep_origin      1348..1936
     misc_feature    2122..2262
                     /locus_tag="/product="
                     /standard_name="bom"
     CDS             complement(2364..2555)
                     /locus_tag="/product=""Rop protein, which maintains
                     plasmids at low copy number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
                     /standard_name="rop"
     terminator      2735..2821
                     /locus_tag="/note=""transcription terminator T1 from the
                     E. coli rrnB ge ne"
                     /standard_name="rrnB T1 terminator"
     misc_feature    2782
                     /locus_tag="/product="
                     /standard_name="A"
     primer_bind     2804..2827
                     /standard_name="oiG17-50"
     misc_feature    complement(2804..2827)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-008"
     misc_feature    2828..5065
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     primer_bind     2828..2910
                     /standard_name="oiG17-014"
     misc_feature    complement(2828..2910)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-015"
     misc_feature    complement(2834..2849)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-67"
     primer_bind     2850..2871
                     /standard_name="oiG17-66"
     RBS             2911..2945
                     /locus_tag="/product="
                     /standard_name="strong RBS"
     source          <2911..>3671
                     /organism
     primer_bind     2911..2940
                     /standard_name="oiG17-001"
     CDS             2946..3671
                     /locus_tag="/note=""sfGFP"
                     /product="/translation=""MSKGEELFTGVVPILVELDGDVNGHKFSVRGEG
                     EGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKD
                     DGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNG
                     IKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYKRP"
                     /standard_name="sfGFP"
     misc_feature    complement(3647..3677)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-002"
     RBS             3678..3699
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
                     /standard_name="RBS"
     source          complement(<3678..>4424)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     3678..3706
                     /standard_name="oiG17-005"
     CDS             3708..4424
                     /codon_start=1
                     /note="mCherry"
                     /translation="MHSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYKRP"
                     /standard_name="mCherry CDS"
     misc_feature    complement(3723..3728)
                     /note="introduction"
                     /note="introduction of two point mutations to disrupt 
                     second inframe RBS in the mCherry gene (2x GAG --> GAA for
                     E)"
                     /standard_name="introduction"
     misc_feature    complement(4403..4433)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-006"
     misc_feature    complement(4412..4433)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-64"
     RBS             4434..4459
                     /standard_name="70k RBS"
     CDS             4460..5044
                     /standard_name="luxI"
     primer_bind     4460..4482
                     /standard_name="oiG17-63"
     misc_feature    4463..4465
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    4466..4468
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4472..4474
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4481..4483
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4484..4486
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    4487..4489
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    4490..4492
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4493..4495
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    4496..4498
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4499..4501
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    4502..4504
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4505..4507
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAA"
     misc_feature    4508..4510
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAA"
     misc_feature    4511..4513
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    4520..4522
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4523..4525
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4526..4528
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4529..4531
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4535..4537
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    4538..4540
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    4541..4543
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    4544..4546
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    4547..4549
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAA"
     misc_feature    4550..4552
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    4553..4555
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    4556..4558
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4559..4561
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAA"
     misc_feature    4568..4570
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4574..4576
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    4580..4582
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    4586..4588
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4592..4594
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4595..4597
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    4598..4600
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAA"
     misc_feature    4601..4603
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    4604..4606
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    4610..4612
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4613..4615
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    4616..4618
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    4622..4624
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    4625..4627
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4628..4630
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    4631..4633
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    4634..4636
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TGC"
     misc_feature    4637..4639
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    4640..4642
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    4643..4645
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    4649..4651
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    4652..4654
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    4655..4657
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4658..4660
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGT"
     misc_feature    4670..4672
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4673..4675
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4676..4678
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    4679..4681
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    4682..4684
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    4685..4687
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGC"
     misc_difference 4687
                     /standard_name="T"
     misc_feature    4688..4690
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    4691..4693
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    4703..4705
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4709..4711
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    4712..4714
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    4718..4720
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4721..4723
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4727..4729
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    4733..4735
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4736..4738
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    4739..4741
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    4745..4747
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    4748..4750
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    4751..4753
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    4754..4756
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4757..4759
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    4763..4765
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4766..4768
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4772..4774
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    4775..4777
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    4778..4780
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    4787..4789
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    4790..4792
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4793..4795
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4796..4798
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAA"
     misc_feature    4799..4801
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4802..4804
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    4811..4813
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    4814..4816
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4820..4822
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4823..4825
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    4832..4834
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4835..4837
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    4841..4843
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    4844..4846
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4847..4849
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    4856..4858
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    4862..4864
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4865..4867
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAG"
     misc_feature    4871..4873
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4874..4876
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    4880..4882
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TAC"
     misc_feature    4883..4885
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    4886..4888
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    4889..4891
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    4892..4894
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    4895..4897
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4898..4900
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    4901..4903
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    4904..4906
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4907..4909
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAA"
     misc_feature    4910..4912
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CGT"
     misc_feature    4913..4915
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    4916..4918
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4919..4921
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAA"
     misc_feature    4925..4927
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4934..4936
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    4937..4939
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TGC"
     misc_feature    4940..4942
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    4946..4948
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4949..4951
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GGT"
     misc_feature    4961..4963
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    4964..4966
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CAC"
     misc_feature    4967..4969
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    4970..4972
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4976..4978
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAC"
     misc_feature    4979..4981
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ACC"
     misc_feature    4985..4987
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TCT"
     misc_feature    4991..4993
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    4994..4996
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    5003..5005
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CCG"
     misc_feature    5006..5008
                     /note="Geneious type: Optimized Codon"
                     /standard_name="ATC"
     misc_feature    5009..5011
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    5018..5020
                     /note="Geneious type: Optimized Codon"
                     /standard_name="TTC"
     misc_feature    5027..5029
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GCG"
     misc_feature    5030..5032
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    5033..5035
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    5036..5038
                     /note="Geneious type: Optimized Codon"
                     /standard_name="AAC"
     misc_feature    5045..5065
                     /standard_name="bbsuffix"
     misc_feature    complement(5047..5065)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG17-53"
ORIGIN      
        1 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
       61 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
      121 acgatttaaa tttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
      181 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
      241 tattcagcgt gaaacgagct gtagccgtcc gcgtctgaac agcaacatgg atgcggatct
      301 gtatggctat aaatgggcgc gtgataacgt gggtcagagc ggcgcgacca tttatcgtct
      361 gtatggcaaa ccggatgcgc cggaactgtt tctgaaacat ggcaaaggca gcgtggcgaa
      421 cgatgtgacc gatgaaatgg tgcgtctgaa ctggctgacc gaatttatgc cgctgccgac
      481 cattaaacat tttattcgca ccccggatga tgcgtggctg ctgaccaccg cgattccggg
      541 caaaaccgcg tttcaggtgc tggaagaata tccggatagc ggcgaaaaca ttgtggatgc
      601 gctggccgtg tttctgcgtc gtctgcatag cattccggtg tgcaactgcc cgtttaacag
      661 cgatcgtgtg tttcgtctgg cccaggcgca gagccgtatg aacaacggcc tggtggatgc
      721 gagcgatttt gatgatgaac gtaacggctg gccggtggaa caggtgtgga aagaaatgca
      781 taaactgctg ccgtttagcc cggatagcgt ggtgacccac ggcgatttta gcctggataa
      841 cctgattttc gatgaaggca aactgattgg ctgcattgat gtgggccgtg tgggcattgc
      901 ggatcgttat caggatctgg ccattctgtg gaactgcctg ggcgaattta gcccgagcct
      961 gcaaaaacgt ctgtttcaga aatatggcat tgataatccg gatatgaaca aactgcaatt
     1021 tcatctgatg ctggatgaat ttttctaata attaattgga caagggtcct tttccgctgc
     1081 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     1141 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     1201 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     1261 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg
     1321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1741 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1981 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2041 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2101 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2161 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2221 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     2281 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2341 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     2401 cagctgcggt aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca
     2461 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     2521 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     2581 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     2641 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     2701 cggcgggggc gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca
     2761 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
     2821 gatgcctgaa ttcgcggccg cttctagaga cctgtaggat cgtacaggtt tacgcaagaa
     2881 aatggtttgt tatagtcgaa taaaccatgg gataaatgct tcaataatat gaggaggtga
     2941 tctagatgag caaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     3001 atggtgatgt taatgggcac aaattttctg tccgtggaga gggtgaaggt gatgctacaa
     3061 acggaaaact cacccttaaa tttatttgca ctactggaaa actacctgtt ccgtggccaa
     3121 cacttgtcac tactctgacc tatggtgttc aatgcttttc ccgttatccg gatcacatga
     3181 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa cgcactatat
     3241 ctttcaaaga tgacgggacc tacaagacgc gtgctgaagt caagtttgaa ggtgataccc
     3301 ttgttaatcg tatcgagtta aagggtattg attttaaaga agatggaaac attcttggac
     3361 acaaactcga gtacaacttt aactcacaca atgtatacat cacggcagac aaacaaaaga
     3421 atggaatcaa agctaacttc aaaattcgcc acaacgttga agatggttcc gttcaactag
     3481 cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
     3541 attacctgtc gacacaatct gtcctttcga aagatcccaa cgaaaagcgt gaccacatgg
     3601 tccttcttga gtttgtaact gctgctggga ttacacatgg catggatgag ctctacaaaa
     3661 ggccttaata agagctcttt gtttaacttt aatgaggagg tatacgcatg catagcaagg
     3721 gcgaagaaga taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg
     3781 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     3841 gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     3901 tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc
     3961 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     4021 aggacggcgg cgtggtgacc gtgacccagg actcctcctt gcaggacggc gagttcatct
     4081 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga
     4141 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg
     4201 agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca
     4261 cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg
     4321 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     4381 gccactccac cggcggcatg gacgagctgt acaagaggcc ttaataagtc gacggggtaa
     4441 cccgtaggta ggggggaaaa tgaccatcat gatcaaaaaa tctgacttcc tggcgatccc
     4501 gtctgaagaa tacaaaggta tcctgtctct gcgttaccag gttttcaaac agcgtctgga
     4561 atgggacctg gttgttgaaa acaacctgga atctgacgaa tacgacaact ctaacgcgga
     4621 atacatctac gcgtgcgacg acaccgaaaa cgtttctggt tgctggcgtc tgctgccgac
     4681 caccggcgac tacatgctga aatctgtttt cccggaactg ctgggtcagc agtctgcgcc
     4741 gaaagacccg aacatcgttg aactgtctcg tttcgcggtt ggtaaaaact cttctaaaat
     4801 caacaactct gcgtctgaaa tcaccatgaa actgttcgaa gcgatctaca aacacgcggt
     4861 ttctcagggt atcaccgaat acgttaccgt tacctctacc gcgatcgaac gtttcctgaa
     4921 acgtatcaaa gttccgtgcc accgtatcgg tgacaaagaa atccacgttc tgggtgacac
     4981 caaatctgtt gttctgtcta tgccgatcaa cgaacagttc aaaaaagcgg ttctgaacta
     5041 ataatactag tagcggccgc tgcag
//
