LOCUS       Exported                2266 bp ds-DNA     circular SYN 02-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 2, 2017 from SnapGene 4.0.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..196
                     /label=p32
                     /note="/ApEinfo_fwdcolor=#f58a5e
                     /ApEinfo_revcolor=#f58a5e"
     misc_feature    68..71
                     /label=NGCG
                     /note="/ApEinfo_fwdcolor=#ffef86
                     /ApEinfo_revcolor=#ffef86"
     misc_feature    70..72
                     /label=NGG
                     /note="/ApEinfo_fwdcolor=#f8d3a9
                     /ApEinfo_revcolor=#f8d3a9"
     misc_feature    79..81
                     /label=NGG
                     /note="/ApEinfo_fwdcolor=#f8d3a9
                     /ApEinfo_revcolor=#f8d3a9"
     misc_feature    197..217
                     /label=BioBrick suffix
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     stem_loop       218..289
                     /label=E. coli his operon terminator
                     /note="/ApEinfo_fwdcolor=#b7e6d7
                     /ApEinfo_revcolor=#b7e6d7"
     stem_loop       226..260
                     /label=Stem loop
                     /note="/ApEinfo_fwdcolor=#75c6a9
                     /ApEinfo_revcolor=#75c6a9"
     misc_feature    complement(353..372)
                     /label=Verification reverse (VR) primer binding site
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     misc_feature    complement(457..1071)
                     /label=rep (pMB1)
                     /note="/ApEinfo_fwdcolor=#f8d3a9
                     /ApEinfo_revcolor=#f8d3a9"
     misc_feature    472
                     /label=ORI
                     /note="/ApEinfo_fwdcolor=#b7e6d7
                     /ApEinfo_revcolor=#b7e6d7"
     stem_loop       1238..1343
                     /label=T0 terminator
                     /note="/ApEinfo_fwdcolor=#d59687
                     /ApEinfo_revcolor=#d59687"
     misc_feature    complement(1356..2015)
                     /label=Chloramphenicol resistance
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     misc_feature    2129..2148
                     /label=Verification forward (VF2) primer binding site
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     stem_loop       complement(2205..2208)
                     /label=end of terminator
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
     stem_loop       complement(2218..2237)
                     /label=Stem loop
                     /note="/ApEinfo_fwdcolor=#b1ff67
                     /ApEinfo_revcolor=#b1ff67"
ORIGIN
        1 gatatgataa gattaatagt tttagctatt aatctttttt tatttttatt taagaatggc
       61 ttaataaagc ggttactttg gatttttgtg agcttggact agaaaaaaac ttcacaaaat
      121 gctatactag gtaggtaaaa aaatattcgg aggaattttg aaatggcaat cgtttcagca
      181 gaaaaattcg taattctact agtagcggcc gctgcagtcc ggcaaaaaag ggcaaggtgt
      241 caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg caggcttcct
      301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc
      481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      901 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1021 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1081 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1141 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1201 gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg
     1261 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     1321 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
     1381 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     1441 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     1501 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     1561 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
     1621 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     1681 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     1741 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     1801 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     1861 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     1921 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     1981 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     2041 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     2101 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat
     2161 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
     2221 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagag
//
