LOCUS       Exported               10349 bp ds-DNA     circular SYN 01-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pRH180
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2017 from SnapGene 4.0.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_RNA        complement(141..403)
                     /label=TracRNA
     operon          join(615..5590,5587..5928,5918..6580)
                     /operon="Cas9, Cas1, Cas2, Csn2"
                     /label=hCas9 operon
     primer_bind     631..652
                     /label=G19
     primer_bind     complement(1280..1300)
                     /label=G20
     primer_bind     2054..2074
                     /label=G23
     primer_bind     complement(6295..6318)
                     /label=G24
     misc_feature    6683..7114
                     /label=Spacer Array
     CDS             7972..8622
                     /codon_start=1
                     /product="CMR"
                     /label=CMR
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
ORIGIN
        1 ctcgtggatt ctgtgatttg gatccttcca gaagtctttt tcttgcactg tttccttttc
       61 tttatgatag tttacgaaat catcctgtgg agcttagtag gtttagcaag atggcagcgc
      121 ctaaatgtag aatgataaaa ggattaagag attaatttcc ctaaaaatga taaaacaagc
      181 gttttgaaag cgcttgtttt tttggtttgc agtcagagta gaatagaagt atcaaaaaaa
      241 gcaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta
      301 tgctgttttg aatggttcca acaagattat tttataactt ttataacaaa taatcaagga
      361 gaaattcaaa gaaatttatc agccataaaa caatacttaa tactatagaa tgataacaaa
      421 ataaactact ttttaaaaga attttgtgtt ataatctatt tattattaag tattgggtaa
      481 tattttttga agagatattt tgaaaaagaa aaattaaagc atattaaact aatttcggag
      541 gtcattaaaa ctattattga aatcatcaaa ctcattatgg atttaattta aactttttat
      601 tttaggaggc aaaaatggat aagaaatact caataggctt agatatcggc acaaatagcg
      661 tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg
      721 gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg
      781 gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga
      841 agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata
      901 gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc
      961 atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct
     1021 atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt
     1081 tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc
     1141 ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat
     1201 ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat
     1261 tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg
     1321 gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt
     1381 ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag
     1441 ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt
     1501 tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc
     1561 ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa
     1621 aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa
     1681 aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta
     1741 tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg
     1801 aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact
     1861 tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca
     1921 atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg
     1981 cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca tttaccccat
     2041 ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga
     2101 caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg
     2161 agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa
     2221 aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa
     2281 atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg
     2341 atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg
     2401 atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct
     2461 tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac
     2521 ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt
     2581 atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg
     2641 gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc
     2701 tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac
     2761 aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag
     2821 gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc
     2881 cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa
     2941 attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc
     3001 ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc
     3061 tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt
     3121 atgatgtcga tcacattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg
     3181 tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag
     3241 tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta
     3301 agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt
     3361 ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg
     3421 atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga
     3481 ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac
     3541 gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg
     3601 ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt
     3661 atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat
     3721 atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag
     3781 agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata
     3841 aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca
     3901 agaaaacaga agtacagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt
     3961 cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata
     4021 gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga
     4081 agttaaaatc cgttaaagag ttactaggga tcacaattat ggaaagaagt tcctttgaaa
     4141 aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca
     4201 ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta
     4261 gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt
     4321 tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac
     4381 aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat
     4441 tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca
     4501 aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga
     4561 cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat
     4621 atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt
     4681 atgaaacacg cattgatttg agtcagctag gaggtgactg atggctggtt ggcgtactgt
     4741 tgtggtaaat acccactcga aattatccta taagaataat catctgattt ttaaggatgc
     4801 ctataaaacg gagctgatcc atttatcaga aattgatatt ttgttattag aaacgaccga
     4861 tattgtcttg tccactatgc tggtaaaacg gctagtggat gagaatgtcc ttgtcatatt
     4921 ctgtgatgat aaacgattac caacagctat gctgatgcct ttttatggtc gtcatgattc
     4981 gagtttacag cttgggaaac aaatgtcctg gtcagaaaca gtcaaatcgc aggtttggac
     5041 gacgattatt gctcaaaaga ttttgaatca atcttgctat ctaggagcat gctcctattt
     5101 tgaaaaatcc caatctatta tggatttata tcatggtttg gaaaattttg atccgagtaa
     5161 tcgagaaggg catgcagcga gaatttattt taatacactt tttgggaacg atttctcaag
     5221 agatttggag catccaatca atgcaggtct ggattatggt tatactttat tattgagtat
     5281 gtttgcgcgt gaagtggttg tgtctggatg tatgactcag tttgggctta aacacgctaa
     5341 tcagtttaat cagttcaatt ttgctagcga tattatggaa ccatttaggc ctttagtgga
     5401 taagattgtt tatgaaaatc gaaatcagcc ttttcccaaa ataaagagag agttatttac
     5461 tttgttttca gatacatttt catataatgg taaagagatg tatctcacga atattattag
     5521 cgattatact aaaaaagttg tcaaagctct gaataatgaa gggaaaggag ttcctgaatt
     5581 taggatatga gttatagata tatgagaatg atacttatgt ttgatatgcc gacggacacc
     5641 gctgaggaac gaaaagccta tcgaaaattt cggaaatttt tacttagtga agggtttatc
     5701 atgcatcaat tttctattta tagtaagttg ctgttgaata atacagctaa caatgccatg
     5761 attggtcggc tgagggagca taatcctaat aaaggaaata ttacattact aacggtcacg
     5821 gaaaaacagt ttgcacgaat gatttattta catggtgaaa gaaataattg tattgcaaac
     5881 tccgatgaaa gacttgtatt tcttggggag gcttttgatg aatcttaatt tttccttact
     5941 agatgaaccg attccattaa gaggcggtac aattcttgtg ctcgaagatg tctgtgtatt
     6001 ttcaaaaata gtgcaatatt gttaccaata tgaggaagat tctgaactta aattttttga
     6061 tcacaagatg aaaacaatca aagaatcaga aatcatgctt gtaacagata ttttaggatt
     6121 tgatgttaac tcctcaacca ttttaaaatt gattcatgca gatttagaat ctcaatttaa
     6181 tgagaaaccc gaagtgaaat cgatgattga caaattggtt gctacgatta cagaactgat
     6241 tgtctttgaa tgcttagaaa atgaattaga tttagagtat gatgaaatca caatcctgga
     6301 attgattaag tccttaggag taaaagtaga aacgcaaagt gatactattt ttgaaaaatg
     6361 tctagagata cttcaaattt tcaaatatct cactaagaaa aagttgctta tttttgtcaa
     6421 tagcggagct tttctaacaa aggatgaagt ggctagttta caagagtata tatcattgac
     6481 aaatttaaca gttctctttt tagaaccacg tgaactatat gattttccgc agtatatttt
     6541 agatgaagat tatttcttaa taactaaaaa tatggtataa tactcttaat aaatgcagta
     6601 atacaggggc ttttcaagac tgaagtctag ctgagacaaa tagtgcgatt acaaaatttt
     6661 ttagacaaaa atagtctacg aggttttaga gctatgctgt tttgaatggt cccaaaactg
     6721 cgctggttga tttcttcttg cgctttttgt tttagagcta tgctgttttg aatggtccca
     6781 aaacttatat gaacataact caatttgtaa aaaagtttta gagctatgct gttttgaatg
     6841 gtcccaaaac aggaatatcc gcaataatta attgcgctct gttttagagc tatgctgttt
     6901 tgaatggtcc caaaacagtg ccgaggaaaa attaggtgcg cttggcgttt tagagctatg
     6961 ctgttttgaa tggtcccaaa actaaatttg tttagcaggt aaaccgtgct ttgttttaga
     7021 gctatgctgt tttgaatggt cccaaaactt cagcacactg agacttgttg agttccatgt
     7081 tttagagcta tgctgttttg aatggtctcc attcaacatt gccgatgata acttgagaaa
     7141 gagggttaat accagcagtc ggataccttc ctattctttc tgttaaagcg ttttcatgtt
     7201 ataataggca aaagaagagt agtgtgatgg aacaaacatt ttttatgatt aagccatatg
     7261 gggttaagca aggggaggta gttggagagg ttttacggtg gattgaacgc ctaagattta
     7321 cgtttaagcg attcgagcta agacaagcta gttcgaaata cttggctaag cacgacgagg
     7381 ccttggtgat aaaccttttg atcctaaact taaagcttac atgacaagtg gtcctgtttt
     7441 aattgggata attcttgggg actaaggtgg ttttgtcttt ttgaaccatg atgagagtaa
     7501 ccaatcctaa ggatgctctt tgcggaacca ttcgtgagaa ttttgctcag gctcctggtg
     7561 atgatggagg tatttttaac atggtgcatg ggtctcattc aagagactct gctaggcgtg
     7621 agatcgctct ttgatctcat caaagtaatc ttggtagctc cgctattctc ttgcaagata
     7681 atccgtaaga aaagataggc aaaggcctac cttttttgtt ataatagaag gaaagactaa
     7741 atgtatagga aaatagaatg aacagtcaag atttaaaaaa acgtcaggag aagattcgta
     7801 atttgcaccc attagttcaa caaacgaaaa ttggataaag tgggatattt ttaaaatata
     7861 tatttatgtt acagtaatat tgacttttaa aaaaggattg attctaatga agaaagcaga
     7921 caagtaagcc tcctaaattc actttagata aaaatttagg aggcatatca aatgaacttt
     7981 aataaaattg atttagacaa ttggaagaga aaagagatat ttaatcatta tttgaaccaa
     8041 caaacgactt ttagtataac cacagaaatt gatattagtg ttttataccg aaacataaaa
     8101 caagaaggat ataaatttta ccctgcattt attttcttag tgacaagggt gataaactca
     8161 aatacagctt ttagaactgg ttacaatagc gacggagagt taggttattg ggataagtta
     8221 gagccacttt atacaatttt tgatggtgta tctaaaacat tctctggtat ttggactcct
     8281 gtaaagaatg acttcaaaga gttttatgat ttataccttt ctgatgtaga gaaatataat
     8341 ggttcgggga aattgtttcc caaaacacct atacctgaaa atgctttttc tctttctatt
     8401 attccatgga cttcatttac tgggtttaac ttaaatatca ataataatag taattacctt
     8461 ctacccatta ttacagcagg aaaattcatt aataaaggta attcaatata tttaccgcta
     8521 tctttacagg tacatcattc tgtttgtgat ggttatcatg caggattgtt tatgaactct
     8581 attcaggaat tgtcagatag gcctaatgac tggcttttat aatatgagat aatgccgact
     8641 gtacttttta cagtcggttt tctaatgtca ctaacctgcc ccgttagttg aagaaggttt
     8701 ttatattaca gctccagatc catatccttc tttttctgaa ccgacttctc ctttttcgct
     8761 tctttattcc aattgcttta ttgacgttga gcctcggaac ccttaacaat cccaaaactt
     8821 gtcgaatggt cggcttaata gctcacgcta tgccgacatt cgtctgcaag tttagttaag
     8881 ggttcttctc aacgcacaat aaattttctc ggcataaatg cgtggtctaa tttttatttt
     8941 taataacctt gatagcaaaa aatgccattc caatacaaaa ccacatacct ataatcgata
     9001 accacataac agtcataaag atcttgcata attcacgctg acctcccaat aactacatgg
     9061 tgttatcggg aggtcagctg ttagcactta tattttgtta ttgttcttcc tcgatttcgt
     9121 ctatcatttt gtgattaatt tctctttttt cttgttctgt taagtcataa agttcactag
     9181 ctaaatactc tttttgtttc caaatataaa aaatttgata gatatattac ggttggatca
     9241 atttctttta agtaatctaa atccccattt tttaatttct ttttagcctc tttaaataat
     9301 cctgaataaa ctaatacctg tttaccttta agtgatttat aaaatgcatc aaagactttt
     9361 tgatttattt attaaataat cactatcttt accagaatac ttagccattt catataattc
     9421 tttattatta ttttgtctta ttttttgaac ttgaacttgt gttatttctg aaatgcccgt
     9481 tacatcacgc cataaatcta accattcttg ttggctaata taatatcttt tatctgtgaa
     9541 atacgattta tttactgcaa ttaacacatg aaaatgagga ttataatcat ctcttttttt
     9601 attatatgta atctctaact tacgaacata tccctttata acactaccta ctttttttct
     9661 ctttataagt tttctaaaag aattattata acgttttatt tcattttcta attcatcact
     9721 cattacatta ggtgtagtca aagttaaaaa gataaactcc tttttctctt gctgcttaat
     9781 atattgcatc atcaaagata aacccaatgc atcttttcta gcttttctcc aagcacagac
     9841 aggacaaaat cgatttttac aagaattagc tttatataat ttctgttttt ctaaagtttt
     9901 atcagctaca aaagacagaa atgtattgca atcttcaact aaatccattt gattctctcc
     9961 aatatgacgt ttaataaatt tctgaaatac ttgatttctt tgttttttct cagtatactt
    10021 ttccatgtta taacacataa aaacaactta gttttcacaa actatgacaa taaaaaaagt
    10081 tgctttttcc cctttctatg tatgtttttt actagtcatt taaaacgata cattaatagg
    10141 tacgaaaaag caactttttt tgcgcttaaa accagtcata ccaataactt aagggtaact
    10201 agcctcgccg gcaatagtta cccttattat caagataaga aagaaaagga tttttcgcta
    10261 cgctcaaatc ctttaaaaaa acacaaaaga ccacattttt taatgtggtc tttattcttc
    10321 aactaaagca cccattagtt caacaaacg
//
