LOCUS       piG17-1-009_Plux        3881 bp    DNA     circular UNA 01-NOV-2017
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...cv-88wu35h
VERSION     urn.local...cv-88wu35h
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     source          <1..2827
                     /organism
                     /label="source"
     terminator      7..101
                     /locus_tag="/note=""transcription terminator from phage
                     lambda"
                     /product
                     /label="lambda t0 terminator"
     CDS             233..1048
                     /locus_tag="/note=""confers resistance to kanamycin in
                     bacteria or G418 (Geneticin®) in eukaryotes"
                     /product="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /label="KanR"
     oriT            1204..1312
                     /locus_tag="/note=""incP origin of transfer"
                     /product
                     /label="oriT"
     rep_origin      1348..1936
                     /locus_tag="/note=""high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of rep lication"
                     /product
                     /label="ori"
     misc_feature    2122..2262
                     /locus_tag="/product="
                     /label="bom"
     CDS             complement(2364..2555)
                     /locus_tag="/product=""Rop protein, which maintains
                     plasmids at low copy number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
                     /label="rop"
     terminator      2735..2821
                     /locus_tag="/note=""transcription terminator T1 from the
                     E. coli rrnB ge ne"
                     /product
                     /label="rrnB T1 terminator"
     DNA_probe_bind  2788..2867
                     /annotation_group="f9f7bb9f-ba1f-4196-9cc7-3b27b9f7359a"
                     /Mismatches=0
                     /label="oiG17-00_LCR_bridge_backbone_PF_Plux"
     misc_feature    2828..2849
                     /created_by="cherasv"
                     /modified_by="cherasv"
                     /label="BioBrick Prefix"
     promoter        2850..2910
                     /created_by="cherasv"
                     /label="Plux "
     DNA_probe_bind  2871..2950
                     /annotation_group="68ac14b0-d3d2-4177-aed1-e8aa20876cca"
                     /Mismatches=0
                     /label="oiG17-00_LCR_bridge_Plux_RBS_azurin"
     source          <2911..>3351
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="CIT135"
                     /db_xref="taxon:287"
                     /label="source Pseudomonas aeruginosa"
     misc_feature    2911..2952
                     /Salis_TIR=32320
                     /created_by="cherasv"
                     /modified_by="cherasv"
                     /label="gcggt+RBS"
     source          <2953..>3342
                     /organism="Pseudomonas aeruginosa"
                     /mol_type="genomic DNA"
                     /strain="CIT135"
                     /db_xref="taxon:287"
                     /label="source Pseudomonas aeruginosa"
     CDS             2953..3342
                     /gene="azu"
                     /note="precursor"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="AAA25730.1"
                     /translation="MLRKLAAVSLLSLLSAPLLAAECSVDIQGNDQMQFNTNAITVDK
                     SCKQFTVNLSHPGNLPKNVMGHNWVLSTAADMQGVVTDGMASGLDKDYLKPDDSRVIA
                     HTKLIGSGEKDSVTFDVSKLKEGEQYMFFCTFPGHSALMKGTLTLK"
                     /label="azu CDS"
     Optimized_Codon 2956..2958
                     /Relative_Adaptiveness="1.0"
                     /Original="GCC"
                     /modified_by="cherasv"
                     /label="GCG"
     Optimized_Codon 2959..2961
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 2965..2967
                     /Relative_Adaptiveness="1.0"
                     /Original="TCG"
                     /label="TCT"
     Optimized_Codon 2968..2970
                     /Relative_Adaptiveness="1.0"
                     /Original="GTG"
                     /label="GTT"
     Optimized_Codon 3007..3009
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     Optimized_Codon 3010..3012
                     /Relative_Adaptiveness="1.0"
                     /Original="GCC"
                     /label="GCG"
     Optimized_Codon 3019..3021
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTT"
     Optimized_Codon 3025..3027
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3028..3030
                     /Relative_Adaptiveness="1.0"
                     /Original="AGC"
                     /label="TCT"
     Optimized_Codon 3034..3036
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3046..3048
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTT"
     Optimized_Codon 3055..3057
                     /Relative_Adaptiveness="1.0"
                     /Original="TCC"
                     /label="TCT"
     Optimized_Codon 3061..3063
                     /Relative_Adaptiveness="1.0"
                     /Original="CCC"
                     /label="CCG"
     Optimized_Codon 3064..3066
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3076..3078
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3082..3084
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTT"
     Optimized_Codon 3088..3090
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3100..3102
                     /Relative_Adaptiveness="1.0"
                     /Original="GTA"
                     /label="GTT"
     Optimized_Codon 3106..3108
                     /Relative_Adaptiveness="1.0"
                     /Original="AGC"
                     /label="TCT"
     Optimized_Codon 3112..3114
                     /Relative_Adaptiveness="1.0"
                     /Original="GCC"
                     /label="GCG"
     Optimized_Codon 3115..3117
                     /Relative_Adaptiveness="1.0"
                     /Original="GCC"
                     /label="GCG"
     Optimized_Codon 3127..3129
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3130..3132
                     /Relative_Adaptiveness="1.0"
                     /Original="GTG"
                     /label="GTT"
     Optimized_Codon 3133..3135
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTT"
     Optimized_Codon 3142..3144
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3148..3150
                     /Relative_Adaptiveness="1.0"
                     /Original="GCT"
                     /label="GCG"
     Optimized_Codon 3151..3153
                     /Relative_Adaptiveness="1.0"
                     /Original="TCC"
                     /label="TCT"
     Optimized_Codon 3154..3156
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3163..3165
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3166..3168
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3175..3177
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3178..3180
                     /Relative_Adaptiveness="1.0"
                     /Original="CCC"
                     /label="CCG"
     Optimized_Codon 3187..3189
                     /Relative_Adaptiveness="1.0"
                     /Original="AGC"
                     /label="TCT"
     Optimized_Codon 3190..3192
                     /Relative_Adaptiveness="1.0"
                     /Original="CGC"
                     /label="CGT"
     Optimized_Codon 3193..3195
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTT"
     Optimized_Codon 3199..3201
                     /Relative_Adaptiveness="1.0"
                     /Original="GCC"
                     /label="GCG"
     Optimized_Codon 3208..3210
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3217..3219
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3220..3222
                     /Relative_Adaptiveness="1.0"
                     /Original="TCG"
                     /label="TCT"
     Optimized_Codon 3223..3225
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3226..3228
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3229..3231
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3235..3237
                     /Relative_Adaptiveness="1.0"
                     /Original="TCG"
                     /label="TCT"
     Optimized_Codon 3238..3240
                     /Relative_Adaptiveness="1.0"
                     /Original="GTG"
                     /label="GTT"
     Optimized_Codon 3250..3252
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTT"
     Optimized_Codon 3253..3255
                     /Relative_Adaptiveness="1.0"
                     /Original="TCC"
                     /label="TCT"
     Optimized_Codon 3256..3258
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3262..3264
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3268..3270
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3271..3273
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3301..3303
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3307..3309
                     /Relative_Adaptiveness="1.0"
                     /Original="TCC"
                     /label="TCT"
     DNA_probe_bind  3312..3391
                     /annotation_group="b54698dc-7e31-4e7b-bade-1915ee0879fc"
                     /Mismatches=0
                     /label="oiG-00_LCR_bridge_azur_bfr"
     Optimized_Codon 3319..3321
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3322..3324
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3337..3339
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3340..3342
                     /Relative_Adaptiveness="1.0"
                     /Original="TGA"
                     /label="TAA"
     misc_feature    <3343..3351
                     /created_by="cherasv"
                     /modified_by="cherasv"
                     /label="SacI_GTC_ext"
     misc_feature    3343..3348
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI, SstI"
                     /note="Geneious type: restriction site"
     misc_feature    3344..3347
                     /Recognition_pattern="AG^CT"
                     /annotation_group="AGCT"
                     /label="AluI"
                     /note="Geneious type: restriction site"
     misc_feature    3352..3377
                     /synthetic_RBS,_Salis_calculator_TIR=52140
                     /created_by="cherasv"
                     /label="RBS"
     CDS             3378..3854
                     /created_by="David"
                     /label="hdBFR"
     Optimized_Codon 3387..3389
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3390..3392
                     /Relative_Adaptiveness="1.0"
                     /Original="ACT"
                     /label="ACC"
     Optimized_Codon 3399..3401
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATC"
     Optimized_Codon 3402..3404
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     Optimized_Codon 3405..3407
                     /Relative_Adaptiveness="1.0"
                     /Original="TAT"
                     /label="TAC"
     Optimized_Codon 3408..3410
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 3420..3422
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 3423..3425
                     /Relative_Adaptiveness="1.0"
                     /Original="GGA"
                     /label="GGT"
     Optimized_Codon 3426..3428
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     Optimized_Codon 3429..3431
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3432..3434
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3435..3437
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTT"
     Optimized_Codon 3438..3440
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCG"
     misc_feature    3440..3443
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3444..3446
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     misc_feature    3448..3453
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI"
                     /note="Geneious type: restriction site"
     misc_feature    3449..3452
                     /Recognition_pattern="GT^AC"
                     /annotation_group="GTAC"
                     /label="RsaI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3453..3455
                     /Relative_Adaptiveness="1.0"
                     /Original="TTT"
                     /label="TTC"
     Optimized_Codon 3456..3458
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3459..3461
                     /Relative_Adaptiveness="1.0"
                     /Original="CAT"
                     /label="CAC"
     misc_feature    3462..3465
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3462..3464
                     /Relative_Adaptiveness="1.0"
                     /Original="GCC"
                     /label="GCG"
     Optimized_Codon 3465..3467
                     /Relative_Adaptiveness="1.0"
                     /Original="CGG"
                     /label="CGT"
     Optimized_Codon 3471..3473
                     /Relative_Adaptiveness="1.0"
                     /Original="TTT"
                     /label="TTC"
     Optimized_Codon 3486..3488
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 3495..3497
                     /Relative_Adaptiveness="1.0"
                     /Original="CTC"
                     /label="CTG"
     Optimized_Codon 3498..3500
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     Optimized_Codon 3501..3503
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3504..3506
                     /Relative_Adaptiveness="1.0"
                     /Original="GTG"
                     /label="GTT"
     Optimized_Codon 3507..3509
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3510..3512
                     /Relative_Adaptiveness="1.0"
                     /Original="TAT"
                     /label="TAC"
     Optimized_Codon 3513..3515
                     /Relative_Adaptiveness="1.0"
                     /Original="CAT"
                     /label="CAC"
     misc_feature    3516..3520
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3519..3521
                     /Relative_Adaptiveness="1.0"
                     /Original="TCC"
                     /label="TCT"
     Optimized_Codon 3522..3524
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     misc_feature    3523..3526
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3525..3527
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3528..3530
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3540..3542
                     /Relative_Adaptiveness="1.0"
                     /Original="GCC"
                     /label="GCG"
     misc_feature    3542..3546
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3543..3545
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3549..3551
                     /Relative_Adaptiveness="1.0"
                     /Original="TAT"
                     /label="TAC"
     Optimized_Codon 3552..3554
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     misc_feature    3553..3556
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3555..3557
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3558..3560
                     /Relative_Adaptiveness="1.0"
                     /Original="CGC"
                     /label="CGT"
     Optimized_Codon 3561..3563
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 3564..3566
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3567..3569
                     /Relative_Adaptiveness="1.0"
                     /Original="TTT"
                     /label="TTC"
     misc_feature    3569..3573
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    3569..3573
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3579..3581
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3582..3584
                     /Relative_Adaptiveness="1.0"
                     /Original="CCA"
                     /label="CCG"
     Optimized_Codon 3588..3590
                     /Relative_Adaptiveness="1.0"
                     /Original="TTA"
                     /label="CTG"
     Optimized_Codon 3591..3593
                     /Relative_Adaptiveness="0.21586"
                     /Original="CAG"
                     /label="CAA"
     misc_feature    3596..3600
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    3596..3600
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3600..3602
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3612..3614
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 3621..3623
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3627..3629
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3645..3647
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     misc_feature    3647..3651
                     /Recognition_pattern="CC^WGG"
                     /annotation_group="CCWGG"
                     /label="BstNI"
                     /note="Geneious type: restriction site"
     misc_feature    3647..3651
                     /Recognition_pattern="^CCWGG"
                     /annotation_group="CCWGG"
                     /label="EcoRII"
                     /note="Geneious type: restriction site"
     misc_feature    3650..3655
                     /Recognition_pattern="RGCGC^Y"
                     /annotation_group="RGCGCY"
                     /label="HaeII"
                     /note="Geneious type: restriction site"
     misc_feature    3651..3654
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3651..3653
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCG"
     Optimized_Codon 3654..3656
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     Optimized_Codon 3657..3659
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3663..3665
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3666..3668
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3672..3674
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3675..3677
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     Optimized_Codon 3678..3680
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 3684..3686
                     /Relative_Adaptiveness="1.0"
                     /Original="GAG"
                     /label="GAA"
     Optimized_Codon 3687..3689
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCG"
     misc_feature    3688..3693
                     /Recognition_pattern="CGAT^CG"
                     /annotation_group="CGATCG"
                     /label="PvuI"
                     /note="Geneious type: restriction site"
     misc_feature    3689..3692
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3690..3692
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 3696..3698
                     /Relative_Adaptiveness="1.0"
                     /Original="TAT"
                     /label="TAC"
     Optimized_Codon 3699..3701
                     /Relative_Adaptiveness="1.0"
                     /Original="GCC"
                     /label="GCG"
     misc_feature    3702..3706
                     /Recognition_pattern="G^ANTC"
                     /annotation_group="GANTC"
                     /label="HinfI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3702..3704
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3705..3707
                     /Relative_Adaptiveness="1.0"
                     /Original="AGC"
                     /label="TCT"
     Optimized_Codon 3711..3713
                     /Relative_Adaptiveness="1.0"
                     /Original="CAT"
                     /label="CAC"
     Optimized_Codon 3714..3716
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3720..3722
                     /Relative_Adaptiveness="1.0"
                     /Original="GTC"
                     /label="GTT"
     Optimized_Codon 3723..3725
                     /Relative_Adaptiveness="1.0"
                     /Original="AGC"
                     /label="TCT"
     Optimized_Codon 3726..3728
                     /Relative_Adaptiveness="1.0"
                     /Original="CGC"
                     /label="CGT"
     Optimized_Codon 3729..3731
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     misc_feature    3737..3740
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3738..3740
                     /Relative_Adaptiveness="1.0"
                     /Original="ATA"
                     /label="ATC"
     misc_feature    3739..3742
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3744..3746
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 3747..3749
                     /Relative_Adaptiveness="1.0"
                     /Original="TTG"
                     /label="CTG"
     Optimized_Codon 3750..3752
                     /Relative_Adaptiveness="1.0"
                     /Original="CGC"
                     /label="CGT"
     Optimized_Codon 3753..3755
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     Optimized_Codon 3762..3764
                     /Relative_Adaptiveness="1.0"
                     /Original="GGC"
                     /label="GGT"
     Optimized_Codon 3765..3767
                     /Relative_Adaptiveness="1.0"
                     /Original="CAT"
                     /label="CAC"
     misc_feature    3769..3772
                     /Recognition_pattern="T^CGA"
                     /annotation_group="TCGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3783..3785
                     /Relative_Adaptiveness="1.0"
                     /Original="ACG"
                     /label="ACC"
     Optimized_Codon 3789..3791
                     /Relative_Adaptiveness="1.0"
                     /Original="CTT"
                     /label="CTG"
     misc_feature    3791..3795
                     /Recognition_pattern="G^GWCC"
                     /annotation_group="GGWCC"
                     /label="AvaII"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3792..3794
                     /Relative_Adaptiveness="1.0"
                     /Original="GAT"
                     /label="GAC"
     misc_feature    3797..3800
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3798..3800
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 3804..3806
                     /Relative_Adaptiveness="1.0"
                     /Original="AAG"
                     /label="AAA"
     Optimized_Codon 3810..3812
                     /Relative_Adaptiveness="1.0"
                     /Original="GGA"
                     /label="GGT"
     Optimized_Codon 3819..3821
                     /Relative_Adaptiveness="1.0"
                     /Original="AAT"
                     /label="AAC"
     Optimized_Codon 3822..3824
                     /Relative_Adaptiveness="1.0"
                     /Original="TAT"
                     /label="TAC"
     misc_feature    3831..3834
                     /Recognition_pattern="GCG^C"
                     /annotation_group="GCGC"
                     /label="HhaI, CfoI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3831..3833
                     /Relative_Adaptiveness="1.0"
                     /Original="GCA"
                     /label="GCG"
     misc_feature    3836..3839
                     /Recognition_pattern="^GATC"
                     /annotation_group="GATC"
                     /label="MboI, Sau3AI"
                     /note="Geneious type: restriction site"
     Optimized_Codon 3837..3839
                     /Relative_Adaptiveness="1.0"
                     /Original="ATT"
                     /label="ATC"
     Optimized_Codon 3840..3842
                     /Relative_Adaptiveness="1.0"
                     /Original="CGC"
                     /label="CGT"
     DNA_probe_bind  3842..40
                     /annotation_group="94c4f70c-4299-48c0-be50-0541bd8650d6"
                     /Mismatches=0
                     /label="oiDG17-00_LCR_bridge_bfr_SF_backbone"
     misc_feature    3851..3854
                     /Recognition_pattern="T^TAA"
                     /annotation_group="TTAA"
                     /label="MseI"
                     /note="Geneious type: restriction site"
     misc_feature    3855..>3881
                     /created_by="cherasv"
                     /modified_by="cherasv"
                     /label="Suffix+TATTTA"
     misc_feature    3856..3861
                     /Recognition_pattern="A^CTAGT"
                     /annotation_group="ACTAGT"
                     /label="SpeI"
                     /note="Geneious type: restriction site"
     misc_feature    3863..3870
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI"
                     /note="Geneious type: restriction site"
     misc_feature    3865..3868
                     /Recognition_pattern="GG^CC"
                     /annotation_group="GGCC"
                     /label="HaeIII"
                     /note="Geneious type: restriction site"
     misc_feature    3870..3875
                     /Recognition_pattern="CTGCA^G"
                     /annotation_group="CTGCAG"
                     /label="PstI"
                     /note="Geneious type: restriction site"
ORIGIN      
        1 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
       61 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
      121 acgatttaaa tttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
      181 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
      241 tattcagcgt gaaacgagct gtagccgtcc gcgtctgaac agcaacatgg atgcggatct
      301 gtatggctat aaatgggcgc gtgataacgt gggtcagagc ggcgcgacca tttatcgtct
      361 gtatggcaaa ccggatgcgc cggaactgtt tctgaaacat ggcaaaggca gcgtggcgaa
      421 cgatgtgacc gatgaaatgg tgcgtctgaa ctggctgacc gaatttatgc cgctgccgac
      481 cattaaacat tttattcgca ccccggatga tgcgtggctg ctgaccaccg cgattccggg
      541 caaaaccgcg tttcaggtgc tggaagaata tccggatagc ggcgaaaaca ttgtggatgc
      601 gctggccgtg tttctgcgtc gtctgcatag cattccggtg tgcaactgcc cgtttaacag
      661 cgatcgtgtg tttcgtctgg cccaggcgca gagccgtatg aacaacggcc tggtggatgc
      721 gagcgatttt gatgatgaac gtaacggctg gccggtggaa caggtgtgga aagaaatgca
      781 taaactgctg ccgtttagcc cggatagcgt ggtgacccac ggcgatttta gcctggataa
      841 cctgattttc gatgaaggca aactgattgg ctgcattgat gtgggccgtg tgggcattgc
      901 ggatcgttat caggatctgg ccattctgtg gaactgcctg ggcgaattta gcccgagcct
      961 gcaaaaacgt ctgtttcaga aatatggcat tgataatccg gatatgaaca aactgcaatt
     1021 tcatctgatg ctggatgaat ttttctaata attaattgga caagggtcct tttccgctgc
     1081 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     1141 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     1201 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     1261 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg
     1321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1741 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1981 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2041 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2101 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2161 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2221 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     2281 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2341 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     2401 cagctgcggt aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca
     2461 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     2521 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     2581 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     2641 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     2701 cggcgggggc gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca
     2761 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
     2821 gatgcctgaa ttcgcggccg cttctagaga cctgtaggat cgtacaggtt tacgcaagaa
     2881 aatggtttgt tatagtcgaa taaaccatgg gcggtcctta tatcatcatc aattcactaa
     2941 gggggttaca ttatggcgga atgctctgtt gacatccagg gtaacgacca gatgcagttc
     3001 aacaccaacg cgatcaccgt tgacaaatct tgcaaacagt tcaccgttaa cctgtctcac
     3061 ccgggtaacc tgccgaaaaa cgttatgggt cacaactggg ttctgtctac cgcggcggac
     3121 atgcagggtg ttgttaccga cggtatggcg tctggtctgg acaaagacta cctgaaaccg
     3181 gacgactctc gtgttatcgc gcacaccaaa ctgatcggtt ctggtgaaaa agactctgtt
     3241 accttcgacg tttctaaact gaaagaaggt gaacagtaca tgttcttctg caccttcccg
     3301 ggtcactctg cgctgatgaa aggtaccctg accctgaaat aagagctcgt caaggaaaag
     3361 acttctagga ggttccaatg aaaggtgaca ccaaagttat caactacctg aacaaactgc
     3421 tgggtaacga actggttgcg atcaaccagt acttcctgca cgcgcgtatg ttcaaaaact
     3481 ggggtctgaa acgtctgaac gacgttgaat accacgaatc tatcgacgaa cacaaacacg
     3541 cggaccgtta catcgaacgt atcctgttcc tggaaggtct gccgaacctg caagacctgg
     3601 gtaaactgaa catcggtgaa gacgttgaag aaatgctgcg ttctgacctg gcgctggaac
     3661 tggacggtgc gaaaaacctg cgtgaagcga tcggttacgc ggactctgtt cacgactacg
     3721 tttctcgtga catgatgatc gaaatcctgc gtgacgaaga aggtcacatc gactggctgg
     3781 aaaccgaact ggacctgatc cagaaaatgg gtctgcaaaa ctacctgcaa gcgcagatcc
     3841 gtgaagaagg ttaatactag tagcggccgc tgcagtattt a
//
