LOCUS       piG17-1-011lib          4041 bp    DNA     circular UNA 24-OCT-2017
DEFINITION  TlpA-RBS lib-pE, const. gfp.
ACCESSION   urn.local...84-84ru6c6
VERSION     urn.local...84-84ru6c6
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      7..101
                     /locus_tag="/note=""transcription terminator from phage
                     lambda"
                     /standard_name="lambda t0 terminator"
     CDS             233..1048
                     /locus_tag="/note=""confers resistance to kanamycin in
                     bacteria or G418 (Geneticin®) in eukaryotes"
                     /product="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
                     /standard_name="KanR"
     oriT            1204..1312
                     /locus_tag="/note=""incP origin of transfer"
                     /standard_name="oriT"
     rep_origin      1348..1936
     misc_feature    2122..2262
                     /locus_tag="/product="
                     /standard_name="bom"
     CDS             complement(2364..2555)
                     /locus_tag="/product=""Rop protein, which maintains
                     plasmids at low copy number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
                     /standard_name="rop"
     terminator      2735..2821
                     /locus_tag="/note=""transcription terminator T1 from the
                     E. coli rrnB ge ne"
                     /standard_name="rrnB T1 terminator"
     misc_feature    2782
                     /locus_tag="/product="
                     /standard_name="A"
     misc_feature    2828..2849
                     /standard_name="BioBrick Prefix 1"
     misc_feature    2850..2853
                     /standard_name="extension GGGC"
     promoter        2854..2905
                     /bound_moiety="TlpA"
                     /note="TlpA Promoter"
                     /standard_name="TlpA Promoter"
     misc_feature    2885..3270
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="ciG17-013 PCR Product 2 (concatenated
                     sequence 2)"
     misc_feature    2885..2906
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    join(3253,2906)
                     /standard_name="RS4 (HindIII)"
     misc_feature    2907..2938
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    <2907..2911
                     /standard_name="reconstituted RS4 (HindIII)"
     RBS             2912..2938
                     /standard_name="Protein E RBS Library"
     primer_bind     2939..2959
                     /standard_name="protein nude (1 F) - Digestion of
                     piG17-1-005"
     CDS             2939..>3211
                     /codon_start=1
                     /translation="MVRWTLWDTLAFLLLLSLLLPSLLIMFIPSTFKRPVSSWKALNL
                     RKTLLMASSVRLKPLNCSRLPCVYAQETLTFLLTQKKTCVKNYVRKE"
                     /standard_name="proteinE phiX174"
     misc_feature    complement(3198..3217)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="protein nude (279 R) - Digestion of
                     piG17-1-005"
     misc_feature    complement(3212..3214)
                     /standard_name="rev compl stop codon"
     misc_feature    complement(3215..3217)
                     /standard_name="rev compl stop codon"
     misc_feature    3218..3252
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     promoter        3218..3252
                     /standard_name="BBa_J23118"
     misc_feature    3253..2906
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of piG17-1-005 (concatenated
                     sequence 1)"
     misc_feature    3253..3270
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    complement(3254..3259)
                     /standard_name="RS3 (SalI)"
     RBS             3260..3294
                     /locus_tag="/product="
                     /standard_name="strong RBS"
     CDS             3295..4020
                     /locus_tag="/note=""sfGFP"
                     /product="/translation=""MSKGEELFTGVVPILVELDGDVNGHKFSVRGEG
                     EGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKD
                     DGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNG
                     IKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYKRP"
                     /standard_name="sfGFP"
     misc_feature    4021..4041
                     /standard_name="BioBrick Suffix"
ORIGIN      
        1 gtcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
       61 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaggggtc cccaataatt
      121 acgatttaaa tttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
      181 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
      241 tattcagcgt gaaacgagct gtagccgtcc gcgtctgaac agcaacatgg atgcggatct
      301 gtatggctat aaatgggcgc gtgataacgt gggtcagagc ggcgcgacca tttatcgtct
      361 gtatggcaaa ccggatgcgc cggaactgtt tctgaaacat ggcaaaggca gcgtggcgaa
      421 cgatgtgacc gatgaaatgg tgcgtctgaa ctggctgacc gaatttatgc cgctgccgac
      481 cattaaacat tttattcgca ccccggatga tgcgtggctg ctgaccaccg cgattccggg
      541 caaaaccgcg tttcaggtgc tggaagaata tccggatagc ggcgaaaaca ttgtggatgc
      601 gctggccgtg tttctgcgtc gtctgcatag cattccggtg tgcaactgcc cgtttaacag
      661 cgatcgtgtg tttcgtctgg cccaggcgca gagccgtatg aacaacggcc tggtggatgc
      721 gagcgatttt gatgatgaac gtaacggctg gccggtggaa caggtgtgga aagaaatgca
      781 taaactgctg ccgtttagcc cggatagcgt ggtgacccac ggcgatttta gcctggataa
      841 cctgattttc gatgaaggca aactgattgg ctgcattgat gtgggccgtg tgggcattgc
      901 ggatcgttat caggatctgg ccattctgtg gaactgcctg ggcgaattta gcccgagcct
      961 gcaaaaacgt ctgtttcaga aatatggcat tgataatccg gatatgaaca aactgcaatt
     1021 tcatctgatg ctggatgaat ttttctaata attaattgga caagggtcct tttccgctgc
     1081 ataaccctgc ttcggggtca ttatagcgat tttttcggta tatccatcct ttttcgcacg
     1141 atatacagga ttttgccaaa gggttcgtgt agactttcct tggtgtatcc aacggcgtca
     1201 gccgggcagg ataggtgaag taggcccacc cgcgagcggg tgttccttct tcactgtccc
     1261 ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg cgaggctggc cgtaggccgg
     1321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1741 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1981 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2041 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2101 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     2161 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     2221 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     2281 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     2341 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     2401 cagctgcggt aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca
     2461 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     2521 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     2581 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     2641 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     2701 cggcgggggc gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca
     2761 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
     2821 gatgcctgaa ttcgcggccg cttctagagg ggctttaatt tgtttgttag ttagtttatt
     2881 tgttggtttg tttgtgttat aatataagct tcacaataag aatttnndgg dggaaaatat
     2941 ggtacgctgg actttgtggg ataccctcgc tttcctgctc ctgttgagtt tattgctgcc
     3001 gtcattgctt attatgttca tcccgtcaac attcaaacgg cctgtctcat catggaaggc
     3061 gctgaattta cggaaaacat tattaatggc gtcgagcgtc cggttaaagc cgctgaattg
     3121 ttcgcgttta ccttgcgtgt acgcgcagga aacactgacg ttcttactga cgcagaagaa
     3181 aacgtgcgtc aaaaattacg tgcggaagga gtaataattg acggctagct cagtcctagg
     3241 tattgtgcta gctgtcgacg ataaatgctt caataatatg aggaggtgat ctagatgagc
     3301 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     3361 aatgggcaca aattttctgt ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc
     3421 acccttaaat ttatttgcac tactggaaaa ctacctgttc cgtggccaac acttgtcact
     3481 actctgacct atggtgttca atgcttttcc cgttatccgg atcacatgaa acggcatgac
     3541 tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat
     3601 gacgggacct acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt
     3661 atcgagttaa agggtattga ttttaaagaa gatggaaaca ttcttggaca caaactcgag
     3721 tacaacttta actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa
     3781 gctaacttca aaattcgcca caacgttgaa gatggttccg ttcaactagc agaccattat
     3841 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg
     3901 acacaatctg tcctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag
     3961 tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaaag gccttaataa
     4021 tactagtagc ggccgctgca g
//
