LOCUS       Exported                8130 bp ds-DNA     circular SYN 01-NOV-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2017 from SnapGene 4.0.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      482..576
                     /label=lambda t0 terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     CDS             complement(597..1256)
                     /label=CmR
                     /note="/ApEinfo_fwdcolor=#b7e6d7
                     /ApEinfo_revcolor=#b7e6d7"
     terminator      1440..1483
                     /label=bacterial terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    complement(1468..1493)
                     /label=SB-prep-2Ea
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     misc_feature    1486..1568
                     /label=geneblock prefix
                     /note="/ApEinfo_fwdcolor=#9eafd2
                     /ApEinfo_revcolor=#9eafd2"
     misc_feature    1486..1507
                     /label=BioBrick prefix
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     misc_feature    1533..5639
                     /label=Cas9
                     /note="/ApEinfo_fwdcolor=#f58a5e
                     /ApEinfo_revcolor=#f58a5e"
     misc_feature    1549..2218
                     /label=PCR1
                     /note="/ApEinfo_fwdcolor=#ffef86
                     /ApEinfo_revcolor=#ffef86"
     misc_feature    2199..2991
                     /label=geneblock EcorI/I473F
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    2872..2877
                     /label=EcoRI
                     /note="/ApEinfo_fwdcolor=#9eafd2
                     /ApEinfo_revcolor=#9eafd2"
     misc_feature    2949..2951
                     /label=I473F ATT->TTT
                     /note="/ApEinfo_fwdcolor=#b1ff67
                     /ApEinfo_revcolor=#b1ff67"
     misc_feature    2972..7236
                     /label=PCR2
                     /note="/ApEinfo_fwdcolor=#b1ff67
                     /ApEinfo_revcolor=#b1ff67"
     misc_feature    5639..6508
                     /label=CRISPR-associated endonuclease Cas1
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    6505..6846
                     /label=CRISPR-associated endoribonuclease Cas2
                     /note="/ApEinfo_fwdcolor=#f8d3a9
                     /ApEinfo_revcolor=#f8d3a9"
     misc_feature    6836..7498
                     /label=CRISPR-associated protein Csn2
                     /note="/ApEinfo_fwdcolor=#ff9ccd
                     /ApEinfo_revcolor=#ff9ccd"
     misc_feature    7214..7588
                     /label=Geneblock XbaI
                     /note="/ApEinfo_fwdcolor=#d59687
                     /ApEinfo_revcolor=#d59687"
     misc_feature    7279..7284
                     /label=XbaI CTA->CTT
                     /note="/ApEinfo_fwdcolor=#b7e6d7
                     /ApEinfo_revcolor=#b7e6d7"
     misc_feature    7538..7543
                     /label=-35
                     /note="/ApEinfo_fwdcolor=#c7b0e3
                     /ApEinfo_revcolor=#c7b0e3"
     misc_feature    7559..7567
                     /label=-10
                     /note="/ApEinfo_fwdcolor=#f8d3a9
                     /ApEinfo_revcolor=#f8d3a9"
     misc_feature    7568..7588
                     /label=BioBrick suffix
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     misc_feature    7579..7600
                     /label=SB-prep-3P-1
                     /note="/ApEinfo_fwdcolor=#f58a5e
                     /ApEinfo_revcolor=#f58a5e"
     terminator      7589..7646
                     /label=his operon terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     rep_origin      complement(7841..299)
                     /direction=LEFT
                     /label=ori
                     /note="/ApEinfo_fwdcolor=#ffef86
                     /ApEinfo_revcolor=#ffef86"
ORIGIN
        1 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
       61 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      121 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      181 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
      241 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
      301 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
      361 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      421 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg
      481 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
      541 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
      601 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
      661 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
      721 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
      781 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
      841 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
      901 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
      961 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     1021 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     1081 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     1141 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     1201 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     1261 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     1321 tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt
     1381 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa
     1441 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct
     1501 tctagagaaa ctttttattt taggaggcaa aaatggataa gaaatactca ataggcttag
     1561 atatcggcac aaatagcgtc ggatgggcgg tgatcactga tgattataag gttccgtcta
     1621 aaaagttcaa ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg
     1681 ctcttttatt tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta
     1741 gaaggtatac acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga
     1801 tggcgaaagt agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag
     1861 acaagaagca tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg
     1921 agaaatatcc aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg
     1981 atttgcgctt aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga
     2041 ttgagggaga tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac
     2101 aaacctacaa tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag
     2161 cgattctttc tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc
     2221 ccggtgagaa gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc
     2281 ctaattttaa atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata
     2341 cttacgatga tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt
     2401 ttttggcagc taagaattta tcagatgcta ttttactttc agatatccta agagtaaata
     2461 ctgaaataac taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc
     2521 aagacttgac tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa
     2581 tcttttttga tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag
     2641 aagaatttta taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat
     2701 tggtgaaact aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta
     2761 ttccccatca aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt
     2821 atccattttt aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgaatacctt
     2881 attatgttgg tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg
     2941 aagaaacatt taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat
     3001 catttattga acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa
     3061 aacatagttt gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg
     3121 ttactgaagg aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg
     3181 atttactctt caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca
     3241 aaaaaataga atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt
     3301 cattaggtac ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg
     3361 aagaaaatga agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg
     3421 agatgattga ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac
     3481 agcttaaacg tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta
     3541 ttagggataa gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca
     3601 atcgcaattt tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa
     3661 aagcacaagt gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta
     3721 gccctgctat taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag
     3781 taatggggcg gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa
     3841 ctcaaaaggg ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag
     3901 aattaggaag tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa
     3961 agctctatct ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata
     4021 ttaatcgttt aagtgattat gatgtcgatc acattgttcc acaaagtttc cttaaagacg
     4081 attcaataga caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg
     4141 ttccaagtga agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca
     4201 agttaatcac tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg
     4261 aacttgataa agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc
     4321 atgtggcaca aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta
     4381 ttcgagaggt taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt
     4441 tccaattcta taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctaa
     4501 atgccgtcgt tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct
     4561 atggtgatta taaagtttat gatgttcgta aaatgattgc taagtctgag caagaaatag
     4621 gcaaagcaac cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa
     4681 ttacacttgc aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg
     4741 gagaaattgt ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc
     4801 cccaagtcaa tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa
     4861 ttttaccaaa aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa
     4921 aatatggtgg ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg
     4981 aaaaagggaa atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattatgg
     5041 aaagaagttc ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag
     5101 ttaaaaaaga cttaatcatt aaactaccta aatatagtct ttttgagtta gaaaacggtc
     5161 gtaaacggat gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa
     5221 gcaaatatgt gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag
     5281 aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta
     5341 ttgagcaaat cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag
     5401 ttcttagtgc atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta
     5461 ttcatttatt tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa
     5521 caattgatcg taaacgatat acgtctacaa aagaagtttt agatgccact cttatccatc
     5581 aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgactgat
     5641 ggctggttgg cgtactgttg tggtaaatac ccactcgaaa ttatcctata agaataatca
     5701 tctgattttt aaggatgcct ataaaacgga gctgatccat ttatcagaaa ttgatatttt
     5761 gttattagaa acgaccgata ttgtcttgtc cactatgctg gtaaaacggc tagtggatga
     5821 gaatgtcctt gtcatattct gtgatgataa acgattacca acagctatgc tgatgccttt
     5881 ttatggtcgt catgattcga gtttacagct tgggaaacaa atgtcctggt cagaaacagt
     5941 caaatcgcag gtttggacga cgattattgc tcaaaagatt ttgaatcaat cttgctatct
     6001 aggagcatgc tcctattttg aaaaatccca atctattatg gatttatatc atggtttgga
     6061 aaattttgat ccgagtaatc gagaagggca tgcagcgaga atttatttta atacactttt
     6121 tgggaacgat ttctcaagag atttggagca tccaatcaat gcaggtctgg attatggtta
     6181 tactttatta ttgagtatgt ttgcgcgtga agtggttgtg tctggatgta tgactcagtt
     6241 tgggcttaaa cacgctaatc agtttaatca gttcaatttt gctagcgata ttatggaacc
     6301 atttaggcct ttagtggata agattgttta tgaaaatcga aatcagcctt ttcccaaaat
     6361 aaagagagag ttatttactt tgttttcaga tacattttca tataatggta aagagatgta
     6421 tctcacgaat attattagcg attatactaa aaaagttgtc aaagctctga ataatgaagg
     6481 gaaaggagtt cctgaattta ggatatgagt tatagatata tgagaatgat acttatgttt
     6541 gatatgccga cggacaccgc tgaggaacga aaagcctatc gaaaatttcg gaaattttta
     6601 cttagtgaag ggtttatcat gcatcaattt tctatttata gtaagttgct gttgaataat
     6661 acagctaaca atgccatgat tggtcggctg agggagcata atcctaataa aggaaatatt
     6721 acattactaa cggtcacgga aaaacagttt gcacgaatga tttatttaca tggtgaaaga
     6781 aataattgta ttgcaaactc cgatgaaaga cttgtatttc ttggggaggc ttttgatgaa
     6841 tcttaatttt tccttactag atgaaccgat tccattaaga ggcggtacaa ttcttgtgct
     6901 cgaagatgtc tgtgtatttt caaaaatagt gcaatattgt taccaatatg aggaagattc
     6961 tgaacttaaa ttttttgatc acaagatgaa aacaatcaaa gaatcagaaa tcatgcttgt
     7021 aacagatatt ttaggatttg atgttaactc ctcaaccatt ttaaaattga ttcatgcaga
     7081 tttagaatct caatttaatg agaaacccga agtgaaatcg atgattgaca aattggttgc
     7141 tacgattaca gaactgattg tctttgaatg cttagaaaat gaattagatt tagagtatga
     7201 tgaaatcaca atcctggaat tgattaagtc cttaggagta aaagtagaaa cgcaaagtga
     7261 tactattttt gaaaaatgtc ttgagatact tcaaattttc aaatatctca ctaagaaaaa
     7321 gttgcttatt tttgtcaata gcggagcttt tctaacaaag gatgaagtgg ctagtttaca
     7381 agagtatata tcattgacaa atttaacagt tctcttttta gaaccacgtg aactatatga
     7441 ttttccgcag tatattttag atgaagatta tttcttaata actaaaaata tggtataata
     7501 ctcttaataa atgcagtaat acaggggctt ttcaagactg aagtctagct gagacaaata
     7561 gtgcgattac tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct
     7621 gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg
     7681 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7741 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7801 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc
     7861 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7921 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7981 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     8041 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8101 aaccccccgt tcagcccgac cgctgcgcct
//
