LOCUS       Exported                2761 bp ds-DNA     circular SYN 14-OCT-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 1, 2017 from SnapGene 4.0.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      538..632
                     /label=lambda t0 terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     CDS             complement(653..1312)
                     /label=CmR
                     /note="/ApEinfo_fwdcolor=#b7e6d7
                     /ApEinfo_revcolor=#b7e6d7"
     terminator      1496..1539
                     /label=bacterial terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    complement(1524..1542)
                     /label=SB-prep-2Ea
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     misc_feature    1542
                     /label=BioBrick prefix
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     misc_feature    1543..1563
                     /label=BioBrick prefix
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     misc_feature    complement(1543..1549)
                     /label=SB-prep-2Ea
                     /note="/ApEinfo_fwdcolor=#85dae9
                     /ApEinfo_revcolor=#85dae9"
     misc_feature    1564..1862
                     /label=pusp45
                     /note="/ApEinfo_fwdcolor=#faac61
                     /ApEinfo_revcolor=#faac61"
     misc_feature    1831..1836
                     /label=-35
                     /note="/ApEinfo_fwdcolor=#d6b295
                     /ApEinfo_revcolor=#d6b295"
     misc_feature    1851..1856
                     /label=-10
                     /note="/ApEinfo_fwdcolor=#ffef86
                     /ApEinfo_revcolor=#ffef86"
     misc_feature    1863..2182
                     /label=array validation
                     /note="/ApEinfo_fwdcolor=#b4abac
                     /ApEinfo_revcolor=#b4abac"
     misc_feature    1958..1989
                     /label=Repeat
                     /note="/ApEinfo_fwdcolor=#b1ff67
                     /ApEinfo_revcolor=#b1ff67"
     misc_feature    1990..1993
                     /label=Repeat
                     /note="/ApEinfo_fwdcolor=#b1ff67
                     /ApEinfo_revcolor=#b1ff67"
     misc_feature    1994..2023
                     /label=20
                     /note="/ApEinfo_fwdcolor=#b7e6d7
                     /ApEinfo_revcolor=#b7e6d7"
     misc_feature    2024..2059
                     /label=Repeat
                     /note="/ApEinfo_fwdcolor=#b1ff67
                     /ApEinfo_revcolor=#b1ff67"
     terminator      2183..2254
                     /label=rrnB T1 terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     misc_feature    2255..2270
                     /label=BioBrick suffix
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     misc_feature    2266..2270
                     /label=SB-prep-3P-1
                     /note="/ApEinfo_fwdcolor=#f58a5e
                     /ApEinfo_revcolor=#f58a5e"
     misc_feature    2271..2287
                     /label=SB-prep-3P-1
                     /note="/ApEinfo_fwdcolor=#f58a5e
                     /ApEinfo_revcolor=#f58a5e"
     misc_feature    2271..2275
                     /label=BioBrick suffix
                     /note="/ApEinfo_fwdcolor=#84b0dc
                     /ApEinfo_revcolor=#84b0dc"
     terminator      2276..2333
                     /label=his operon terminator
                     /note="/ApEinfo_fwdcolor=#c6c9d1
                     /ApEinfo_revcolor=#c6c9d1"
     rep_origin      complement(2528..355)
                     /direction=LEFT
                     /label=ori
                     /note="/ApEinfo_fwdcolor=#ffef86
                     /ApEinfo_revcolor=#ffef86"
ORIGIN
        1 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
       61 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      121 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      181 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      241 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      301 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
      361 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
      421 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
      481 ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt
      541 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
      601 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
      661 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
      721 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
      781 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
      841 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
      901 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
      961 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     1021 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1081 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1141 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1201 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1261 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     1321 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     1381 tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa
     1441 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc
     1501 agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta
     1561 gagccgggta tatttactaa tcgctggaca aggcttttta caacaattat tattgtgacc
     1621 gcttttgaag tttttagtgc aatcattatg acagcttttg gatttgccca acttcagttt
     1681 atcaaatttg ttgtttacca gttagcgcct acacttttgc tcaatattat cttagctgta
     1741 gccttacaat tccctttaga aatcttttac agattaaaga aaagtcatgt aagatacaat
     1801 tagaaagtgt tttgtaatca taaagaaata ttaaggtggg gtaggaatag tataatatgt
     1861 ttaataaatg cagtaataca ggggcttttc aagactgaag tctagctgag acaaatagtg
     1921 cgattacgaa attttttaga caaaaatagt ctacgaggtt ttagagctat gctgttttga
     1981 atggtcccaa aacatttcaa ccttaatagt agacaacgca ggagttttag agctatgctg
     2041 ttttgaatgg tcccaaaaca acattgccga tgataacttg agaaagaggg ttaataccag
     2101 cagtcggata ccttcctatt ctttctgtta aagcgttttc atgttataat aggcaaaaga
     2161 agagtagtgt gatggaacaa accaaataaa acgaaaggct cagtcgaaag actgggcctt
     2221 tcgttttatc tgttgtttgt cggtgaacgc tctctactag tagcggccgc tgcagtccgg
     2281 caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc
     2341 gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2401 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2461 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2521 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2581 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2641 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2701 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2761 t
//
