LOCUS       piG17-002_regula        6722 bp    DNA     circular UNA 01-NOV-2017
DEFINITION  pSEVA361.
ACCESSION   urn.local...p-88x719z
VERSION     urn.local...p-88x719z
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
FEATURES             Location/Qualifiers
     misc_feature    1..4692
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    1..21
                     /note="Geneious type: Suffix"
                     /standard_name="BioBrick prefix"
     source          complement(<23..>778)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(23..778)
                     /codon_start=1
                     /product="LuxR-Barcode"
                     /note="luxR (C0062)"
                     /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLA
                     IIYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAV
                     NKKSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPL
                     IVPSLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHL
                     TNAQMKLNTTNRCQSISKAILTGAIDCPYFKN*"
                     /standard_name="LuxR-Barcode CDS"
     RBS             779..814
                     /standard_name="100k RBS"
     gap             850..919
     primer_bind     868..887
                     /standard_name="oDG432"
     promoter        920..954
                     /standard_name="BBa_J23118"
     CDS             987..3429
                     /standard_name="BBa_K1847016"
     gene            993..2646
                     /standard_name="lldP"
     gene            2647..3422
                     /standard_name="lldR"
     misc_feature    2834..2836
                     /note="Geneious type: Optimized Codon"
                     /standard_name="RS destroyed"
     source          <3430..>3437
                     /organism
     terminator      3438..3471
                     /standard_name="B1002 Terminator"
     source          <3472..>3479
                     /organism
     promoter        3480..3514
                     /standard_name="BBa_J23104"
     misc_feature    3515..3520
                     /standard_name="1-RS1 (NcoI) "
     RBS             3521..3552
                     /standard_name="10k RBS"
     CDS             3553..4668
                     /standard_name="tlpA-CDS"
     CDS             <3931..>3936
                     /codon_start=1
                     /standard_name="tlpA-CDS"
     misc_feature    3931..3933
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GTT"
     misc_feature    3934..3936
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAT"
     CDS             <4345..>4350
                     /codon_start=1
                     /standard_name="tlpA-CDS"
     misc_feature    4345..4347
                     /note="Geneious type: Optimized Codon"
                     /standard_name="CTG"
     misc_feature    4348..4350
                     /note="Geneious type: Optimized Codon"
                     /standard_name="GAA"
     misc_feature    4672..4692
                     /standard_name="BioBrick Suffix"
     source          <4693..>6722
                     /organism
     primer_bind     4693..4717
                     /standard_name="oiG17-00_antibiotic_forward_correct_2"
     primer_bind     4693..4717
                     /standard_name="oiG17-00_antibiotic_SF_forward_correct_2"
     terminator      4699..4793
                     /locus_tag="/product="
                     /standard_name="lambda t0 terminator|terminator"
     CDS             4944..5603
                     /locus_tag="/product=""
                     /translation=""MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITA
                     F
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
                     /standard_name="chloramphenicol_acetyltransferase(CAT)_ORF
                     Selectab le marker|CDS"
     misc_feature    5617..5862
                     /locus_tag="/product="
                     /standard_name="oriT|misc_feature"
     primer_bind     5627..5696
                     /standard_name="oDG417"
     misc_feature    complement(5639..5656)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oDG414"
     primer_bind     5657..5678
                     /standard_name="oDG413"
     rep_origin      5907..6452
     unsure          complement(6459..6462)
                     /locus_tag="/note=""CAAAA > CAAA in sequencing 14.11.2014"
     terminator      complement(6630..6716)
                     /locus_tag="/note=""transcription terminator T1 from the
                     E. coli rrnB ge ne"
                     /standard_name="rrnB T1 terminator"
     misc_feature    complement(6699..6722)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG1700_ori_isolation_PF_reverse"
     misc_feature    complement(6699..6722)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="oiG1700_ori_isolation_reverse"
ORIGIN      
        1 gaattcgcgg ccgcttctag agttattaat ttttaaagta tgggcaatca attgctcctg
       61 ttaaaattgc tttagaaata ctttggcagc ggtttgttgt attgagtttc atttgcgcat
      121 tggttaaatg gaaagtgaca gtacgctcac tgcaacctaa tatttttgaa atatcccaag
      181 agctttttcc ttcgcatgcc cacgctaaac attctttttc tcttttggtt aaatcgttgt
      241 ttgatttatt atttgctata tttatttttc gataattatc aactagagaa ggaacaatta
      301 atggtatgtt catacacgca tgtaaaaata aactatctat atagttgtct ttttctgaat
      361 gtgcaaaact aagcattccg aagccattgt tagccgtatg aatagggaaa ctaaacccag
      421 tgataagacc tgatgttttc gcttctttaa ttacatttgg agatttttta tttacagcat
      481 tgttttcaaa tatattccaa ttaattggtg aatgattgga gttagaataa tctactatag
      541 gatcatattt tattaaatta gcgtcatcat aatattgcct ccatttttta gggtaattat
      601 ctaggattga aatatcagat ttaaccatag aatgaggata aatgatcgcg agtaaataat
      661 attcacaatg taccatttta gtcatatcag ataagcattg attaatatca ttattgcttc
      721 tacaagcttt aattttatta attattctgt atgtgtcgtc ggcatttatg tttttcatgt
      781 tgttccctcc tctgttagct gcgtgtttat tcctgctagc actgtaccta ggactgagct
      841 agccgtcaaa ggttctgtta agtaactgaa cccaatgtcg ttagtgacgc ttacctctta
      901 agaggtcact gacctaacat tgacggctag ctcagtccta ggtattgtgc tagcgagctc
      961 tactagagaa agaggagaaa tactagctag agatgaatct ctggcaacaa aactacgatc
     1021 ccgccgggaa tatctggctt tccagtctga tagcatcgct tcccatcctg tttttcttct
     1081 ttgcgctgat taagctcaaa ctgaaaggat acgtcgccgc ctcgtggacg gtggcaatcg
     1141 cccttgccgt ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg
     1201 tttatggttt cttctacggg ttgtggccca tcgcgtggat cattattgca gcggtgttcg
     1261 tctataagat ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct attctttcga
     1321 taacccctga ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc gcgttccttg
     1381 aaggagccgc aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg
     1441 gttttaaacc gctgtacgcc gccgggctgt gcctgattgt taacaccgcg ccagtggcat
     1501 ttggtgcgat gggcattcca atcctggttg ccggacaggt aacaggtatc gacagctttg
     1561 agattggtca gatggtgggg cggcagctac cgtttatgac cattatcgtg ctgttctgga
     1621 tcatggcgat tatggacggc tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg
     1681 cgggcggctc gtttgccatc gctcagtacc ttagctctaa cttcattggg ccggagctgc
     1741 cggacattat ctcttcgctg gtatcactgc tctgcctgac gctgttcctc aaacgctggc
     1801 agccagtgcg tgtattccgt tttggtgatt tgggggcgtc acaggttgat atgacgctgg
     1861 cccacaccgg ttacactgcg ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga
     1921 cagctaccgt aacactgtgg agtatcccgc cgtttaaagc cctgttcgca tcgggtggcg
     1981 cgctgtatga gtgggtgatc aatattccgg tgccgtacct cgataaactg gttgcccgta
     2041 tgccgccagt ggtcagcgag gctacagcct atgccgccgt gtttaagttt gactggttct
     2101 ctgccaccgg caccgccatt ctgtttgctg cactgctctc gattgtctgg ctgaagatga
     2161 aaccgtctga cgctatcagc accttcggca gcacgctgaa agaactggct ctgcccatct
     2221 actccatcgg tatggtgctg gcattcgcct ttatttcgaa ctattccgga ctgtcatcaa
     2281 cactggcgct ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg
     2341 gctggctggg ggtattcctg accgggtcgg atacctcatc taacgccctg ttcgccgcgc
     2401 tgcaagccac cgcagcacaa caaattggcg tctctgatct gttgctggtt gccgccaata
     2461 ccaccggtgg cgtcaccggt aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg
     2521 cggtaggcct ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga
     2581 tcttcacctg tatagtgggc gtgatcacca cgcttcaggc ttatgtctta acgtggatga
     2641 ttccttaatg attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc
     2701 gctgattgat gaaaaaaacc tggaagcggg catgaagttg cccgctgagc gccaactggc
     2761 gatgcaactc ggcgtatcac gtaattcact gcgcgaggcg ctggcaaaac tggtgagtga
     2821 aggcgtgctg ctctctcgac gcggcggcgg gacgtttatt cgctggcgtc atgacacatg
     2881 gtcggagcaa aacatcgtcc agccgctaaa aacactgatg gccgatgatc cggattacag
     2941 tttcgatatt ctggaagccc gctacgccat tgaagccagc accgcatggc atgcggcaat
     3001 gcgcgccaca cctggcgaca aagaaaagat tcagctttgc tttgaagcaa cgctaagtga
     3061 agacccggat atcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc
     3121 acataacatc gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt
     3181 gaagcatagc cgtcagcgga tgtatctggt gccaccggtt ttttcacaac tgaccgaaca
     3241 acatcaggct gtcattgacg ccatttttgc cggtgatgct gacggggcgc gtaaagcaat
     3301 gatggcgcac cttagttttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg
     3361 ccacgcacgg attacccgcc tgcccggtga gcataatgag cattcgaggg agaaaaacgc
     3421 atgataatat actagagcgc aaaaaacccc gcttcggcgg ggttttttcg ctactagagt
     3481 tgacagctag ctcagtccta ggtattgtgc tagcccatgg tgacaacacc ctcagatcgg
     3541 acagggggat acatgcgtcc ggcgacatac gaaccagaac agattattga agcagggctg
     3601 gccctacaag ctgaaggacg gaatatcacc gggttcgcac tacgtaacca ggtgggtggc
     3661 ggcaatccga cacgtctccg ccagatatgg gacgaatacc aggcttcaca gagcacggtc
     3721 gtcactgaac ccgttgccga gctgccagtg gaagtggctg aagaagtgaa ggccgtctcc
     3781 gccgcgctgt ccgaacgcat cacccagctg gcgacagaac tgaatgacaa ggcggtccgg
     3841 gctgctgaac gccgggttgc ggaagtcacg cgtgctgccg gtgaacagac cgcacaggca
     3901 gagcgggagc tggccgacgc cgcgcagaca gttgatgacc tggaagaaaa actggatgaa
     3961 cttcaggaca gatatgacag tttgacgctg gcgctggagt cagaacgttc actgcgtcag
     4021 cagcatgatg tggagatggc ccagctgaaa gagcgtcttg cagccgctga agagaatacc
     4081 cgtcagcgag aggaacggta tcaggagcag aagacagtgc tacaggatgc gcttaatgcg
     4141 gagcaggcac agcacaaaaa cacgcgggaa gacctacaga aacgactgga gcaaatttct
     4201 gccgaagcta atgcgcgtac agaagaactg aagtctgaac gcgataaagt caatactctc
     4261 cttacccgcc ttgaatcgca ggaaaatgcg ctggcctcag aacgtcagca gcatctggcc
     4321 acccgcgaaa cgcttcagca acgcctggaa caggccatcg ctgacacgca ggcgcgcgcc
     4381 ggtgagattg cacttgaacg tgacagagtc agcagcctca ccgcaaggct ggaatcgcag
     4441 gaaaaggcct cctcggagca actggtgcgt atgggcagtg aaatagccag tctgacagag
     4501 cgttgcacac agctggaaaa ccagcgtgat gatgcccgtc tggagacgat gggggagaaa
     4561 gaaacggtcg cggcactgcg tggtgaggct gaagccctga agcgtcagaa ccagtcactg
     4621 atggcggcgc tttcaggcaa taaacagacc ggtggccaga atgcgtaata atactagtag
     4681 cggccgctgc aggtcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     4741 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccagggg
     4801 tccccaataa ttacgattta aattggcgaa aatgagacgt tgatcggcac gtaagaggtt
     4861 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
     4921 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     4981 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     5041 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     5101 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     5161 ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     5221 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     5281 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     5341 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     5401 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     5461 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     5521 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     5581 atgagtggca gggcggggcg taatttgact tttgtccttt tccgctgcat aaccctgctt
     5641 cggggtcatt atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt
     5701 ttgccaaagg gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat
     5761 aggtgaagta ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct
     5821 ggcggtgctc aacgggaatc ctgctctgcg aggctggccg taggccggcc ctagaaatat
     5881 tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt aatctcttgc
     5941 tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg agctaccaac
     6001 tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt cctttcagtt
     6061 tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac cagtggctgc
     6121 tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa
     6181 ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
     6241 ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga
     6301 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg
     6361 cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt
     6421 gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctctcacttc
     6481 cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt aagccatttc
     6541 cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatat
     6601 ccggcgcgcc cagctgtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac
     6661 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
     6721 ct
//
